Ọnụ ego efu China High Fidelity Real Time PCR Sybr Green Qpcr Mix with Low Rox+ Buffer
Nkọwa ngwa:
Usoro mmeghachi omume pụrụ iche na arụmọrụ Taq DNA Polymerase dị elu na-eme ka mmeghachi omume PCR nwee nrụpụta nkwalite dị elu, nkọwapụta na nghọta.
Ezi mma a pụrụ ịdabere na ya na ezigbo kredit bụ ụkpụrụ anyị, nke ga-enyere anyị aka n'ọkwa dị elu.Na-erube isi n'ụkpụrụ gị nke "mma 1st, onye zụrụ ahịa" maka ọnụ ahịa efu China High Fidelity Real Time PCR Sybr GreenQpcrGwakọta ya na obere nchekwa Rox + dị ala, ọmarịcha mma, ọnụ ahịa asọmpi, nnyefe ngwa ngwa na ọrụ a pụrụ ịdabere na ya, jiri nwayọ mee ka anyị mata ọnụọgụ gị n'okpuru ụdị nha ọ bụla ka anyị nwee ike ịgwa gị otu a.
Ezi mma a pụrụ ịdabere na ya na ezigbo kredit bụ ụkpụrụ anyị, nke ga-enyere anyị aka n'ọkwa dị elu.Na-erube isi n'ụkpụrụ gị nke "ịdị mma nke mbụ, onye zụrụ ahịa" makaChina Taq DNA Polymerase, Qpcr, Na elu ogbako, ọkachamara imewe otu na nlezianya àgwà akara usoro, dabeere n'etiti- ruo elu-ọgwụgwụ akara dị ka anyị ahịa n'ọnọdu, anyị ngwọta na-ngwa ngwa na-ere n'elu European na American ahịa na anyị onwe anyị ụdị dị ka n'okpuru Deniya, Qingsiya na Yisilanya.
Nkọwapụta
50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns
Ngwa ngwa
| Akụkụ I | Ihe nchekwa P1 |
| Ihe nchekwa P2 | |
| 6 × DNA na-ebufe ihe nchekwa | |
| Akụkụ II | 2× Akwukwo PCR MfeTMNgwakọta (UNG) |
Atụmatụ & uru
∎ Ọ dịghị ọcha DNA na-ewe oge yana ọnụ
■ Ihe dị obere
∎ Mfe - enwere ike idobe ihe nlele ahụ na mmeghachi omume PCR ma ọ bụ mmeghachi omume lysis na-egbutughị ma ọ bụ egweri
∎ 2 × Mix, na-ebelata mperi mperi ihe nlele na oge nkwadebe usoro mmeghachi omume
■ Enwere ike ịmechaa nkwadebe ngwa ngwa n'ime nkeji 10, enwere ike mechaa mmeghachi omume PCR n'ime opekata mpe nkeji iri ise.
∎ Enwere ike mechaa mmeghachi omume nnabata dị elu na efere PCR 96 nke ọma
Ngwa ngwa
Ngwa: njirimara gbanwetụrụ na mkpụrụ ndụ ihe nketa, genotyping, wdg.
Ihe atụ: akwukwo osisi
Usoro onunu ogwu: dayameta 2-3mm (usoro ozugbo), dayameta 5-7mm (usoro lysis)
Oke nchọpụta: iberibe ebumnuche ≤1kb
Usoro ọrụ
Electropherogram
Izu abụọ nke PCR Mix nchoputa nchọpụta n'okpuru ọnọdụ nke PCR amplification ngwaahịa contamination. A pụrụ ịdabere na ezi àgwà na ezigbo kredit eguzo bụ ụkpụrụ anyị, nke ga-enyere anyị aka n'ọkwa dị elu.Na-erube isi n'ụkpụrụ gị nke "mma 1st, onye zụrụ ahịa" maka ọnụ ahịa efu China High Fidelity Real Time PCR Sybr GreenQpcrGwakọta ya na obere nchekwa Rox + dị ala, ọmarịcha mma, ọnụ ahịa asọmpi, nnyefe ngwa ngwa na ọrụ a pụrụ ịdabere na ya, jiri nwayọ mee ka anyị mata ọnụọgụ gị n'okpuru ụdị nha ọ bụla ka anyị nwee ike ịgwa gị otu a.
Ọnụ ego efuChina Taq DNA Polymerase, Qpcr, Na elu ogbako, ọkachamara imewe otu na nlezianya àgwà akara usoro, dabeere n'etiti-na elu-ọgwụgwụ akara dị ka anyị ahịa n'ọnọdu, anyị ngwọta na-ngwa ngwa na-ere n'elu European na American ahịa na anyị onwe anyị ụdị dị ka n'okpuru Deniya, Qingsiya na Yisilanya.
