wepụ mkpokọta RNA
Ụlọ ọrụ anyị na-agbaso ụkpụrụ bụ isi nke "Ịdịmma nwere ike ịbụ ndụ nke nzukọ gị, na aha nwere ike ịbụ mkpụrụ obi ya" makawepụ mkpokọta RNA, Daalụ maka iwere oge bara uru gị iji gaa na anyị ma nọrọ maka inwe nkwado dị mma na gị.
Ụlọ ọrụ anyị na-agbaso ụkpụrụ bụ isi nke "Ịdịmma nwere ike ịbụ ndụ nke nzukọ gị, na aha nwere ike ịbụ mkpụrụ obi ya" makawepụ mkpokọta RNA, Ụlọ ọrụ anyị na-ekwe nkwa: ọnụ ahịa ezi uche dị na ya, obere oge mmepụta na ọrụ ahịa na-eju afọ, anyị na-anabatakwa gị ka ị gaa na ụlọ ọrụ anyị n'oge ọ bụla ịchọrọ.A sị na anyị nwere azụmahịa dị ụtọ na ogologo okwu ọnụ !!!
Nkọwapụta
Nkwadebe 50, 200 Preps
Ngwa ngwa
| Ihe nchekwa RL1, ihe nchekwa RL2 |
| Ihe nchekwa RW1, ihe nchekwa RW2 |
| ddH na-efu RNase2O, kọlụm na-ehicha DNA |
| RNA-naanị kọlụm |
Atụmatụ & uru
■ Ọ dịghị mkpa ichegbu onwe gị maka mbibi RNA;Sistemụ niile bụ RNase-Free
∎ Wepu DNA nke ọma site na iji kọlụm na-ehicha DNA
■ Wepu DNA na-agbakwunyeghị DNA
■ A na-arụ ọrụ niile dị mfe na ọnụ ụlọ okpomọkụ
■ Enwere ike ịrụcha ọrụ ngwa ngwa n'ime nkeji iri atọ
■ Nchekwa-ọ dịghị ihe reagent organic achọrọ
■ Ịdị ọcha dị elu -OD260 / 280≈1.8-2.1
Ngwa ngwa
Ọ dabara maka mmịpụta na ịdị ọcha nke mkpokọta RNA site na anụ ahụ dị ọhụrụ ma ọ bụ oyi kpọnwụrụ dị iche iche ma ọ bụ sel emebere.
Ngwa ngwaahịa
∎ Ngwa mgbada ala: njikọ cDNA nke mbụ, RT-PCR, cloning molecular, Northern Blot, wdg.
■ Ihe nlele: anụ ahụ anụmanụ, mkpụrụ ndụ omenala
■ Usoro onunu ogwu: Tissues 10-20mg, Sel (2-5) × 106
∎ Oke kachasị ike ijikọ DNA nke kọlụm nchacha: 80 μg
■ Olu olu: 50-200 μl
Usoro ọrụ
Eserese
Ngwakọta Mkpokọta RNA nke anụmanụ na-agwọ 20mg
Ihe nlele òké ọhụrụ, were 5% RNA 1% agar sachara ọcha
Glycogel electrophoresis
1: Spleen 2: Akụrụ
3: Imeju 4: Obi
Nchekwa na ndụ nchekwa
Enwere ike ịchekwa ngwa ahụ maka ọnwa 24 na okpomọkụ nke ụlọ (15-25 ℃) ma ọ bụ 2-8 ℃ maka ogologo oge.Enwere ike ịchekwa ihe nchekwa RL1 na 4 ℃ maka otu ọnwa ka ịgbakwunye β-mercaptoethanol (nhọrọ).
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