Ahụmahụ nchịkwa nchịkwa bara ụba nke ukwuu yana naanị otu onye na-eweta ihe ngosi na-eme ka mkpa dị mkpa nke nkwurịta okwu nzukọ na nghọta anyị dị mfe maka atụmanya gị maka Factory made hot-sale China High Efficiency and Stability for Ivd Use One-Step Probe Rt-QpcrKit V2, Anyị na-achọ n'ihu iji wuo njikọ dị mma ma na-enyere aka na ndị niile na-eweta n'ime ụwa.Anyị ji obi ụtọ na-anabata gị ka ị na-akpọtụrụ anyị ka ị malite mkparịta ụka maka otu anyị nwere ike isi webata nke a.
Ahụmahụ nchịkwa ọrụ bara ụba nke ukwuu yana naanị otu ụdị onye na-eweta ọrụ na-eme ka mkpa nkwukọrịta ọgbakọ dị na nghọta anyị dị mfe maka atụmanya gị maka.China Taq DNA Polymerase, Qpcr, Ha na-eṅomi siri ike ma na-akwalite nke ọma n'ụwa nile.Ọ dịghị mgbe ọ bụla na-apụ n'anya isi ọrụ n'ime ngwa ngwa, ọ bụ a ga-egbo gị mkpa nke kediegwu ezigbo mma.Na-eduzi ụkpụrụ nke Prudence, Efficiency, Union and Innovation.ụlọ ọrụ ahụ.gbasie mbọ ike ịgbasa azụmaahịa mba ụwa, bulie nzukọ ya elu.rofit ma welie ọnụ ọgụgụ mbupụ ya.Anyị nwere obi ike na anyị na-achọ inwe atụmanya na-egbuke egbuke na ikesa anyị n'ụwa niile n'afọ ndị na-abịa.

Nkọwapụta

50 × 20μl rxns, 200 × 20μl rxns, 1000 × 20μl rxns, 2000 × 20μl rxns.

2X Real PCR EasyTM Mix-Taqman nke Real Time PCR EasyTM-Taqman kit na-enye bụ usoro premix ọhụrụ nke na-eji nyocha fluorescent akọwapụtara maka mmeghachi omume nkwalite PCR Real Time, nke nwere ike melite nkọwapụta ngwaahịa na mmetụta mmeghachi omume.A na-enye ROX ka ọ bụrụ ihe eji agba agba n'ime.

2X Real PCR EasyTM Mix-Taqman nwere mmalite mmalite Taq DNA Polymerase pụrụ iche nke Foregene.N'iji ya na enzymes Taq nkịtị, ọ nwere uru dị na nrụpụta nkwalite dị elu, ikike nkwalite siri ike yana ọnụ ọgụgụ na-adaghị adaba.Ọ nwere ike belata mmụba na-abụghị nke akọwapụtara ma melite izi ezi nke PCR.

Ngwa ngwa

Atụmatụ & uru

∎ Mfe—2X PCR Ngwakọta iji belata njehie nnwale na oge ọrụ

∎ Specific—nkwadebe nke kachasị na enzyme Taq mmalite na-ekpo ọkụ nwere ike igbochi nkwalite na-abụghị nke akọwapụtara na nhazi ọkwa mbụ.

∎ Mmetụta dị elu-nwere ike ịchọpụta obere ndebiri nke ndebiri

∎ Ọdịiche dị mma—dakọtara na ọtụtụ ngwa PCR ọnụ ọgụgụ dị adị

Ngwa ngwa

qPCR nyocha

Usoro ọrụ

Eserese

Nchekwa na Shelf ndụ

Ekwesịrị ịchekwa ngwa a n'ebe dịpụrụ adịpụ na ọkụ ma debe ya na -20 ℃.Ọ bụrụ na-eji ugboro ugboro, ọ nwekwara ike na-echekwara na 4 ℃ n'ihi na a obere oge (10 ụbọchị) .The n'ezie ukwuu oru nlekọta ahụmahụ na dị nnọọ otu onye na-eweta ihe nlereanya na-eme ka ọkpụrụkpụ mkpa nke nzukọ nkwurịta okwu na anyị mfe nghọta nke gị na-atụ anya maka Factory mere na-ekpo ọkụ-sale China High Efficiency na Stability maka Ivd Jiri One-Nzọụkwụ Nyocha Rt-QpcrKit V2, Anyị na-achọ n'ihu iji wuo njikọ dị mma ma na-enyere aka na ndị niile na-eweta n'ime ụwa.Anyị ji obi ụtọ na-anabata gị ka ị na-akpọtụrụ anyị ka ị malite mkparịta ụka maka otu anyị nwere ike isi webata nke a.
Factory mere ọkụ-ereChina Taq DNA Polymerase, Qpcr, Ha na-eme ihe ngosi siri ike ma na-akwalite nke ọma n'ụwa nile.Ọ dịghị mgbe ọ bụla na-apụ n'anya isi ọrụ n'ime ngwa ngwa, ọ bụ a ga-egbo gị mkpa nke kediegwu ezigbo mma.Na-eduzi ụkpụrụ nke Prudence, Efficiency, Union and Innovation.ụlọ ọrụ ahụ.gbasie mbọ ike ịgbasa azụmaahịa mba ụwa, bulie nzukọ ya elu.rofit ma welie ọnụ ọgụgụ mbupụ ya.Anyị nwere obi ike na anyị na-achọ inwe atụmanya na-egbuke egbuke na ikesa anyị n'ụwa niile n'afọ ndị na-abịa.

Enweghị mgbama nkwalite

1.Taq DNA Polymerase na ngwa ahụ na-efunahụ ọrụ ya n'ihi nchekwa na-ezighị ezi ma ọ bụ njedebe nke ngwa ahụ.
Nkwanye: Kwado ọnọdụ nchekwa nke ngwa ahụ;tinyeghachi Taq DNA Polymerase kwesịrị ekwesị na sistemu PCR ma ọ bụ zụta ngwa PCR Real Time ọhụrụ maka nnwale ndị metụtara ya.

2.E nwere ọtụtụ ihe mgbochi nke Taq DNA Polymerase na DNA template.
Aro: Megharịa ndebiri ma ọ bụ belata ọnụọgụ ndebiri ejiri.

3.The Mg2 + ịta adịghị adabara.
Nkwanye: Mg2 + ntinye nke the2 × Real PCR Mix anyị na-enye bụ 3.5mM.Agbanyeghị, maka ụfọdụ primers na ndebiri pụrụ iche, ntinye Mg2+ nwere ike ịdị elu.Ya mere, ị nwere ike tinye MgCl2 ozugbo iji bulie mkpokọta Mg2+.A na-atụ aro ka ịbawanye Mg2+ 0.5mM oge ọ bụla maka njikarịcha.

4.The PCR amplification ọnọdụ adịghị adabara, na primer usoro ma ọ bụ itinye uche na-ekwesịghị ekwesị.
Aro: gosi izi ezi nke usoro nke primer ma emebiela primer;Ọ bụrụ na mgbama nkwuwapụta adịghị mma, gbalịa iwetulata okpomọkụ na-ekpo ọkụ ma mezie ntinye nke primer nke ọma.

5. Ọnụ ọgụgụ nke template dị ntakịrị ma ọ bụ nke ukwuu.
Nkwanye: Mee template linearization gradient dilution, wee họrọ mkpokọta template nwere mmetụta PCR kacha mma maka nnwale PCR oge.

NTC nwere oke fluorescence uru

1.Reagent mmetọ mere n'oge ọrụ.
Nkwanye: Dochie na ọhụrụ reagents maka ezigbo oge PCR nnwale.

2.Contamination mere n'oge nkwadebe nke usoro mmeghachi omume PCR.
Nkwanye: Were usoro nchebe dị mkpa mgbe ị na-arụ ọrụ, dị ka: iyi uwe latex, iji ntu pipette nwere nzacha, wdg.

3.The primers na-eweda n'ala, na mmebi nke primers ga-eme ka ndị na-abụghị kpọmkwem amplification.
Aro: Jiri SDS-PAGE electrophoresis chọpụta ma ọ̀tụ̀tụ̀tụ̀ ihe ndị e ji emepụta ihe na-emebi emebi, ma jiri primers ọhụrụ dochie ha maka nnwale PCR Real Time.

Primer dimer ma ọ bụ nkwalite na-abụghị nke akọwapụtara

1.The Mg2 + ịta adịghị adabara.
Nkwanye: Mg2 + ntinye uche nke 2 × Real PCR EasyTM Mix anyị na-enye bụ 3.5 mM.Agbanyeghị, maka ụfọdụ primers na ndebiri pụrụ iche, ntinye Mg2+ nwere ike ịdị elu.Ya mere, ị nwere ike tinye MgCl2 ozugbo iji bulie mkpokọta Mg2+.A na-atụ aro ka ịbawanye Mg2+ 0.5mM oge ọ bụla maka njikarịcha.

2.The PCR annealing okpomọkụ dị oke ala.
Aro: Mee ka PCR annealing okpomọkụ site 1℃ ma ọ bụ 2℃ oge ọ bụla.

3.The PCR ngwaahịa dị ogologo.
Nkwanye: Ogologo ngwaahịa PCR Real Time kwesịrị ịdị n'etiti 100-150bp, ọ bụghị karịa 500bp.

4.The primers na-eweda n'ala, na nbibi nke primers ga-eduga na ọdịdị nke kpọmkwem mmụba.
Aro: Jiri SDS-PAGE electrophoresis chọpụta ma ọ̀tụ̀tụ̀tụ̀ ihe ndị e ji emepụta ihe na-emebi emebi, ma jiri primers ọhụrụ dochie ha maka nnwale PCR Real Time.

5.The PCR usoro na-ekwesịghị ekwesị, ma ọ bụ usoro dị oke obere.
Aro: Sistemụ mmeghachi omume PCR pere mpe ga-eme ka nchọta nke ọma belata.Ọ kacha mma iji usoro mmeghachi omume nke akụrụngwa PCR ọnụọgụ tụrụ aro iji megharịa nnwale PCR Real Time.

Nkwughachi nke ụkpụrụ ọnụọgụgụ adịghị mma

1. The ngwá bụ malfunctioning.
Aro: Enwere ike inwe mperi n'etiti oghere PCR ọ bụla nke ngwaọrụ ahụ, na-ebute mmeghari na-adịghị mma n'oge njikwa okpomọkụ ma ọ bụ nchọpụta.Biko lelee dị ka ntuziaka nke ngwa kwekọrọ.

2.The sample ịdị ọcha adịghị mma.
Nkwanye: Ihe nlele na-adịghị ọcha ga-eduga na mmeghari nke nnwale ahụ na-adịghị mma, nke gụnyere ịdị ọcha nke template na primers.Ọ kacha mma imegharị ndebiri ahụ, yana SDS-PAGE na-asachapụ ihe ndị ahụ.

3.The PCR usoro nkwadebe na oge nchekwa dị ogologo.
Aro: Jiri ezigbo oge PCR usoro maka PCR nnwale ozugbo nkwadebe, na ahapụla ya n'akụkụ ruo ogologo oge.

4.The PCR amplification ọnọdụ adịghị adabara, na primer usoro ma ọ bụ itinye uche na-ekwesịghị ekwesị.
Aro: gosi izi ezi nke usoro nke primer ma emebiela primer;Ọ bụrụ na mgbama nkwuwapụta adịghị mma, gbalịa iwetulata okpomọkụ na-ekpo ọkụ ma mezie ntinye nke primer nke ọma.

5.The PCR usoro na-ekwesịghị ekwesị, ma ọ bụ usoro dị oke obere.
Aro: Sistemụ mmeghachi omume PCR pere mpe ga-eme ka nchọta nke ọma belata.Ọ kacha mma iji usoro mmeghachi omume nke akụrụngwa PCR ọnụọgụ tụrụ aro iji megharịa nnwale PCR Real Time.