Isi mmalite ụlọ ọrụ High Fidelity Hotstart Taq Mix PCR Kit PCR Master Mix 2 × A8 Fasthifi PCR Mastermix
Anyị na-emesi nkwalite na-ewebata ọhụrụ ngwọta n'ime ahịa nanị banyere kwa afọ maka Factory isi iyi High Fidelity Hotstart Taq Mix PCR Kit PCR Master Mix 2 × A8 Fasthifi PCR Mastermix , We're devoted to provide skilled purification technology and options for you personally!
Anyị na-emesi nkwalite na iwebata ngwọta ọhụrụ n'ime ahịa dị ihe dị ka afọ ọ bụla makaChina PCR Kit na PCR, Ruo ugbu a, a na-emelite ndepụta ngwaahịa mgbe niile ma dọta ndị ahịa si gburugburu ụwa.A na-enwetakarị eziokwu dị omimi na webụsaịtị anyị ma ndị otu anyị na-ere ahịa ga-enye gị ezigbo ndụmọdụ ndụmọdụ.Ha ga-enyere gị aka ịghọta nke ọma banyere ngwongwo anyị ma mee mkparịta ụka afọ ojuju.A na-anabatakwa ụlọ ọrụ na ụlọ ọrụ mmepụta ihe na Brazil n'oge ọ bụla.Na-atụ anya inweta ajụjụ gị maka nkwado ọ bụla nwere obi ụtọ.
Akwụkwọ ntuziaka:
Anyị na-emesi nkwalite na-ewebata ọhụrụ ngwọta n'ime ahịa nanị banyere kwa afọ maka Factory isi iyi High Fidelity Hotstart Taq Mix PCR Kit PCR Master Mix 2 × A8 Fasthifi PCR Mastermix , We're devoted to provide skilled purification technology and options for you personally!
Isi mmalite ụlọ ọrụChina PCR Kit na PCR, Ruo ugbu a, a na-emelite ndepụta ngwaahịa mgbe niile ma dọta ndị ahịa si gburugburu ụwa.A na-enwetakarị eziokwu dị omimi na webụsaịtị anyị ma ndị otu anyị na-ere ahịa ga-enye gị ezigbo ndụmọdụ ndụmọdụ.Ha ga-enyere gị aka ịghọta nke ọma banyere ngwongwo anyị ma mee mkparịta ụka afọ ojuju.A na-anabatakwa ụlọ ọrụ na ụlọ ọrụ mmepụta ihe na Brazil n'oge ọ bụla.Na-atụ anya inweta ajụjụ gị maka nkwado ọ bụla nwere obi ụtọ.
Ntuziaka nyocha nsogbu
The following is an analysis of the problems that may be encountered in the extraction of plant genomic DNA, hoping to be helpful to your experiments. In addition, for other experimental or technical problems other than operation instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us: 028-83360257 or E-mali: Tech@foregene.com.
Mkpụrụ dị ala ma ọ bụ enweghị DNA
A na-enwekarị ọtụtụ ihe na-emetụta mkpụrụ nke DNA genomic, gụnyere isi iyi nke sample, afọ nke sample, ọnọdụ nchekwa nke sample, na ọrụ.
Enweghị ike ịnweta DNA genomic n'oge mmịpụta
1. A na-echekwa ihe nlele anụ ahụ na-ekwesịghị ekwesị ma ọ bụ chekwaa ya ogologo oge, na-ebute mmebi nke DNA genomic.
Nkwanye: Chekwaa nlele anụ ahụ na nitrogen mmiri mmiri ma ọ bụ -20°C;nwaa iji ihe nlele anakọtara ọhụrụ maka mmịpụta DNA genomic.
2. Obere ihe nlele nwere ike ime ka a ghara iwepụta DNA genomic kwekọrọ.
Aro: Maka ihe nlele anụ ahụ nke echekwara ogologo oge ma ọ bụ nwere mmebi DNA nke genomic siri ike, enwere ike ịbawanye ọnụọgụ anụ ahụ nke ọma iji wepụta DNA mkpụrụ ndụ ihe nketa.Enwere ike ikpebi ọnụọgụ nke nlele ahụ dịka mkpa DNA si dị, mana ihe nlele ọhụrụ ekwesịghị ịfe 100mg, ihe nlele akọrọ ekwesịghị ịfe 30mg.
3. A naghị etinye ihe nlele ahụ na mmiri mmiri nitrogen ma ọ bụ tinye ya ogologo oge mgbe mmiri mmiri gachara.
Aro: N'oge mmịpụta DNA, ihe nlele ahụ kwesịrị ka ejiri nitrogen mmiri mee ala nke ọma iji mebie mgbidi cell;mgbe ị na-egweri, biko nyefee ntụ ntụ ntụ ntụ na PL1 preheated na 65°C ozugbo enwere ike (ozugbo ntụ ntụ ala gbazere, DNA genomic ga-amalite ịda ngwa ngwa).
4. Nchekwa na-ezighi ezi nke Foregene Protease na-ebute ọrụ belata ma ọ bụ adịghị arụ ọrụ.
Nkwanye: Kwenye ọnọdụ nchekwa nke Foregene Protease ma ọ bụ dochie ya na Foregene Protease ọhụrụ maka enzymatic hydrolysis.
5. A na-echekwa ngwa ahụ n'ụzọ na-ekwesịghị ekwesị ma ọ bụ chekwaa ya ogologo oge, na-eme ka ụfọdụ ihe dị na ngwa ahụ daa.
Nkwanye: Zụrụ ngwa ihe mmịpụta DNA genomic osisi ọhụrụ maka ọrụ ndị metụtara ya.
6. Iji ngwa na-ekwesịghị ekwesị.
Aro: Zụrụ ngwa nkewapụ DNA osisi nke ewepụtara maka nlele maka mmịpụta na sachaa DNA genomic osisi.
7. Buffer WB na-agbakwunyeghị ammiri ara ehi ethanol.
Nkwanye: Gbaa mbọ hụ na ịtinye oke ethanol zuru oke na Buffer WB.
8. Awụpụghị eluent ahụ n'ime akpụkpọ ahụ silica nke ọma.
Aro: Tinye eluent ahụ ebugoro ọkụ na 65℃ịdaba n'etiti akpụkpọ anụ silica gel, ma hapụ ya na ụlọ okpomọkụ maka nkeji 5 iji mee ka arụmọrụ elution dịkwuo elu.
Mwepụta iji nweta DNA genomic dị ala
1. A na-echekwa ihe nlele ahụ na-ekwesịghị ekwesị ma ọ bụ chekwaa ya ogologo oge, na-ebute mmebi nke DNA genomic.
Nkwanye: Chekwaa nlele anụ ahụ na -20℃;gbalịa iji ihe nlele anụ ahụ anakọtara ọhụrụ maka mmịpụta DNA genomic.
2. Ọ bụrụ na ọnụọgụ anụ ahụ dị ntakịrị, DNA genomic amịpụtara ga-adị ntakịrị.
Aro: Ụfọdụ ihe atụ osisi nwere mmiri, dị ka ihe ọkụkụ dị n'ime mmiri dị ka algae, wdg, enwere ike ịbawanye dose ahụ nke ọma ma ọ bụ mee ka mmiri kpoo ntakịrị tupu ọrụ ahụ.
3. A naghị ejikọta ihe atụ ndị ahụ nke ọma na mmiri mmiri nitrogen ma ọ bụ hapụ ya na ụlọ okpomọkụ ruo ogologo oge mgbe egwerichara.
Aro: Mmiri nitrogen egweri ga-ezuru, na sample cell mgbidi kwesịrị ka agbajikwa dị ka o kwere mee;ozugbo egwerichara, a ga-ebufe ntụ ntụ ntụ na 65℃Buffer PL1 preheated maka nzọụkwụ ọzọ.
4. Anaghị eji ngwa ziri ezi.
Nkwanye: Jiri ngwa nkewapụta DNA nke osisi raara onwe ya nye wepụta na sachaa DNA genomic osisi.
5. Nchekwa na-ezighi ezi nke Foregene Protease na-ebute ọrụ belata ma ọ bụ adịghị arụ ọrụ.
Nkwanye: Kwenye ọnọdụ nchekwa nke Foregene Protease ma ọ bụ dochie ya na Foregene Protease ọhụrụ maka enzymatic hydrolysis.
6. Eluent nsogbu
Nkwanye: Biko jiri Buffer EB maka elution;Ọ bụrụ na ị na-eji ddH2O ma ọ bụ eluent ndị ọzọ, hụ na pH nke eluent dị n'etiti 7.0-8.5.
7. Anaghị agbapụta eluent nke ọma
Aro: Biko tinye dobe elution n'etiti akpụkpọ anụ silica ma hapụ ya na ụlọ okpomọkụ maka nkeji 5 iji mee ka ọrụ elution dịkwuo elu.
8. Olu eluent dị obere
Aro: Biko jiri eluent maka genomic DNA elution dị ka ntuziaka si dị, opekata mpe, ọ bụghị ihe na-erughị 100μl.
DNA genomic ewepụtara na ịdị ọcha dị ala
Ịdị ọcha dị ala nke DNA genomic ga-eduga na ọdịda ma ọ bụ mmetụta na-adịghị mma nke nnwale ndị dị n'okpuru ala, dị ka: enweghị ike igbutu enzyme ahụ, na PCR enweghị ike ịnweta mpempe mkpụrụ ndụ e lekwasịrị anya.
1. Mmetọ protein dị iche iche, mmetọ RNA.
Nyocha: A naghị eji Buffer PW sachaa kọlụm;Ejighị Buffer PW sachaa kọlụm na ọsọ centrifugation ziri ezi.
Aro: gbalịa hụ na ọ dịghị mmiri ozuzo na elu mgbe a na-agafe na kọlụm;jide n'aka na ị ga-eji Buffer PW sachaa kọlụm nchacha dịka ntuziaka ahụ siri dị, na enweghị ike ịhapụ usoro a.
2. Mmetọ ion adịghị ọcha.
Nyocha: E wepụrụ kọlụm ịsa ahụ Buffer WB ma ọ bụ saa ya naanị otu ugboro, butere mmetọ ionic fọdụrụnụ.
Nkwanye: Gbaa mbọ hụ na ị na-eji Buffer WB sachaa ugboro abụọ dịka ntuziaka siri dị ka iwepu ion fọdụrụ ka o kwere mee.
3. RNase ofufe.
Nyocha: Exogenous RNase na-agbakwunye na nchekwa;Arụ ọrụ ịsa ahụ na-ezighi ezi na Buffer PW ga-ebute RNase fọdụrụnụ ma metụta ọrụ nnwale RNA mgbada, dị ka ntụgharị in vitro.
Aro: Foregene usoro nucleic acid mmịpụta ngwa nwere ike wepu RNA na-enweghị mgbakwunye RNase, na ihe niile reagents na Plant DNA Isolation Kit adịghị mkpa RNase;jide n'aka na ị ga-eji Buffer PW sachaa kọlụm nchacha dịka ntuziaka ahụ siri dị, na enweghị ike ịhapụ usoro a.
4. Ethanol fọdụrụnụ.
Nyocha: Mgbe ejiri Buffer WB sachaa kọlụm nchacha ahụ, ọ nweghị centrifugation tube efu emere.
Nkwanye: Soro ntuziaka maka centrifugation tube efu kwesịrị ekwesị.
Akwụkwọ ntuziaka:
Akwụkwọ ntuziaka ngwa mwepu DNA nke osisi
