Nkwanye ụlọ ọrụ China M-Mlv Reverse Transcriptase, Rnase H Minus, 2 X Sybr Green Mix
Nkọwa ngwa:
Foreasy M-MLV Reverse Transcriptase bụ transcriptase ọhụrụ reverse egosipụtara na nje bacteria E. coli mebere site na iji teknụzụ nchikota mkpụrụ ndụ ihe nketa.Ọ bụ polymerase DNA recombinant nke na-emepụta eriri DNA na-akwado ya site na RNA, DNA, ma ọ bụ RNA: DNA ngwakọ.Ọ nweghị ọrụ RNase H, nkwụsi ike siri ike, njikọ RNA siri ike, yana nghọta dị elu.
"Dabere na ahịa ụlọ ma gbasaa azụmahịa mba ofesi" bụ atụmatụ nkwalite anyị maka ụlọ ọrụ mmepụta ihe China M-MlvReverse Transcriptase, Rnase H Minus, 2 X Sybr Green Mix, Biko na-eche nnọọ free na-agwa anyị maka nzukọ.anyị kwenyere na anyị ga-eso ndị ahịa anyị niile kerịta ahụmịhe azụmaahịa kacha mma.
"Dabere na ahịa ụlọ ma gbasaa azụmahịa nke mba ofesi" bụ atụmatụ nkwalite anyị makaChina Mmlv H Minus, Reverse Transcriptase, Ugbu a, anyị emeela mmekọrịta siri ike na ogologo oge yana nnukwu ụlọ ọrụ dị ukwuu n'ime azụmahịa a esenidụt.Ngwa ngwa na ọkachamara mgbe ọrịre ahịa wetara site na ndị ndụmọdụ anyị nwere obi ụtọ ndị na-azụ anyị.Enwere ike iziga gị ozi zuru oke na parampat sitere na ngwa ahịa maka nkwenye zuru oke ọ bụla.Enwere ike ibunye ihe nlele efu na ụlọ ọrụ lelee ụlọ ọrụ anyị.n Portugal maka mkparita uka na-nabata mgbe niile.Na-atụ anya ịnweta ajụjụ pịnye gị wee wulite mmekọrịta nkwado ogologo oge.
Nkọwapụta
5ml, 10ml, 40ml
PCR Hero (Ya na Dye) ngwa mmịpụta gụnyere ọgbọ ọhụrụ pụrụ iche nke Foregene Taq-Foregene Taq Hero, dNTP kachasị mma, MgCl2, ihe nchekwa mmeghachi omume, ihe nkwalite mmeghachi omume PCR, njikarịcha na ihe nkwụsi ike.Usoro 2 × PCR HeroTM Mix (ede) nwere nnabata dị elu nye ndị na-egbochi PCR karịa usoro PCR Mix nkịtị, ma nwee ike ịnagide PCR nkwalite dị iche iche dị mgbagwoju anya.Usoro mmeghachi omume pụrụ iche na Taq Hero dị elu na-eme ka mmeghachi omume PCR nwee arụmọrụ nkwalite dị elu, nkọwapụta na uche.
Ngwa ngwa
| 0.1U Taq Hero/μl |
| 400μM dNTPs |
| 20mM Tris-HCl (pH8.8) |
| 100mM KCl |
| 5mM mgCl2 |
| Ndị ọzọ stabilizers na enhancers |
| Ihe nchekwa DNA na-ebu |
Atụmatụ & uru
■ Ikwesị ntụkwasị obi dị elu: 6 ugboro nke enzyme Taq nkịtị;
■ Ọsọ nkwuwa okwu ngwa ngwa: ọsọ nkwalite bụ 5-10sec/kb, nke bụ 6-12 ugboro nke enzyme Taq nkịtị;
∎ Mgbanwe nke ndebiri: Ọ nwere ike ịkwalite ndebiri DNA dị mgbagwoju anya dị elu nke ọmaGC ọdịnaya;
■ Ịrụ ọrụ nkwalite dị elu: okirikiri mmụba dị ala karịa enzyme Taq nkịtị;
■ Nkwekọrịta gburugburu ebe obibi siri ike: etinye ya na 37 ° C maka otu izu, na-ejigide ihe karịrị 90% ọrụ;
■ Ọ nwere 5′ → 3′ DNA polymerase ọrụ na 5′→3′ exonuclease ọrụ, na-enweghị 3′ →5′ exonuclease ọrụ.
■ A na-ejikọta ngwa ahụ na Electrophoresis Loading Buffer, mgbe PCR gwụchara, enwere ike ibunye ihe nlele ahụ ozugbo maka electrophoresis;ọrụ ahụ dị mfe ma dị mfe.
Ngwa ngwa
Mmụba PCR nke iberibe DNA, akara DNA, usoro DNA, PCR tinyere A ọdụ
Nchekwa na Shelf ndụ
Enwere ike ịchekwa ya ogologo oge na -20 Celsius;maka ugboro ugboro, ọ ga-echekwara na 4°C maka obere oge.” Dabere na ụlọ ahịa na ịgbasa overseas azụmahịa” is our enhancement strategy for Factory Supply China M-Mlv Reverse Transcriptase, Rnase H Minus, 2 X Sybr Green Mix, Biko na-eche nnọọ free na-agwa anyị okwu maka nzukọ.anyị kwenyere na anyị ga-eso ndị ahịa anyị niile kerịta ahụmịhe azụmaahịa kacha mma.
Nkwanye ụlọ ọrụChina Mmlv H Minus, Reverse Transcriptase, Anyị ugbu a wulitela mmekọrịta siri ike na ogologo oge yana nnukwu ụlọ ọrụ dị ukwuu n'ime azụmahịa a esenidụt.Ngwa ngwa na ọkachamara mgbe ọrịre ahịa wetara site na ndị ndụmọdụ anyị nwere obi ụtọ ndị na-azụ anyị.Enwere ike iziga gị ozi zuru oke na parampat sitere na ngwa ahịa maka nkwenye zuru oke ọ bụla.Enwere ike ibunye ihe nlele efu na ụlọ ọrụ lelee ụlọ ọrụ anyị.n Portugal maka mkparita uka na-nabata mgbe niile.Na-atụ anya ịnweta ajụjụ pịnye gị wee wulite mmekọrịta nkwado ogologo oge.
