Anyị na-enye gị mgbe niile otu n'ime ndị ahịa kacha nwee akọ na uche, yana ụdị atụmatụ na ụdị dị iche iche nwere ihe kacha mma.Ndị a atumatu na-agụnye availability nke ahaziri designs na ọsọ na izipu maka Fixed Competitive Price Lab Consumables Universal Nucleic Acid Extraction Test Vials Kits, Ngwaahịa anyị na ngwọta na-atọ ụtọ na ọmarịcha ewu ewu n'etiti ndị na-azụ anyị.Anyị na-anabata atụmanya, mkpakọrịta ụlọ ọrụ na ezigbo ndị enyi si n'akụkụ niile nke ụwa gị ka ị kpọtụrụ anyị ma chọọ nkwado maka uru otu.
Anyị na-enye gị mgbe niile otu n'ime ndị ahịa kacha nwee akọ na uche, yana ụdị atụmatụ na ụdị dị iche iche nwere ihe kacha mma.Atụmatụ ndị a gụnyere ịnweta atụmatụ ahaziri ahazi nwere ọsọ na izipu makaMwepụta Acid Nucleic Asịd nke China Universal na Ihe Mwepụta Nucleic Acid, Onye ọ bụla ahịa afọ ojuju bụ ihe mgbaru ọsọ anyị.Anyị na-achọ nkwado ogologo oge na onye ahịa ọ bụla.Iji mezuo nke a, anyị na-akwalite ịdịmma anyị ma na-ebuga ọrụ ndị ahịa pụrụ iche.Nnọọ na ụlọ ọrụ anyị, anyị na-atụ anya ka anyị na gị kwadoo.
Akwụkwọ ntuziaka:

Anyị na-enye gị mgbe niile otu n'ime ndị ahịa kacha nwee akọ na uche, yana ụdị atụmatụ na ụdị dị iche iche nwere ihe kacha mma.Ndị a atumatu na-agụnye availability nke ahaziri designs na ọsọ na izipu maka Fixed Competitive Price Lab Consumables Universal Nucleic Acid Extraction Test Vials Kits, Ngwaahịa anyị na ngwọta na-atọ ụtọ na ọmarịcha ewu ewu n'etiti ndị na-azụ anyị.Anyị na-anabata atụmanya, mkpakọrịta ụlọ ọrụ na ezigbo ndị enyi si n'akụkụ niile nke ụwa gị ka ị kpọtụrụ anyị ma chọọ nkwado maka uru otu.
Ọnụ ahịa asọmpi edobereMwepụta Acid Nucleic Asịd nke China Universal na Ihe Mwepụta Nucleic Acid, Onye ọ bụla ahịa afọ ojuju bụ ihe mgbaru ọsọ anyị.Anyị na-achọ nkwado ogologo oge na onye ahịa ọ bụla.Iji mezuo nke a, anyị na-akwalite ịdịmma anyị ma na-ebuga ọrụ ndị ahịa pụrụ iche.Nnọọ na ụlọ ọrụ anyị, anyị na-atụ anya ka anyị na gị kwadoo.

Ntuziaka nyocha nsogbu

Ihe na-esonụ bụ nyocha nke nsogbu ndị nwere ike izute na mmịpụta nke DNA/RNA viral, na-atụ anya na ọ ga-enyere gị aka na nnwale gị.Na mgbakwunye, maka nsogbu nnwale ma ọ bụ teknụzụ ndị ọzọ karịa ntuziaka ọrụ na nyocha nsogbu, anyị ewepụtala nkwado teknụzụ iji nyere gị aka.Ọ bụrụ na ị nwere mkpa ọ bụla, biko kpọtụrụ anyị: 028-83360257 ma ọ bụ E-mail:

Tech@foregene.com.

Enweghị mmịpụta nucleic acid ma ọ bụ mkpụrụ nucleic acid dị ala

A na-enwekarị ọtụtụ ihe na-emetụta arụmọrụ mgbake, dị ka: sample ọdịnaya nucleic acid, usoro ọrụ, elution volume, wdg.

Nyocha nke ihe na-akpatakarị:

1. A na-eme centrifugation ice bat ma ọ bụ obere okpomọkụ (4 ° C) n'oge usoro ahụ.

Aro: Na-arụ ọrụ na ụlọ okpomọkụ (15-25ºC) n'ime usoro dum, emela ice bat na obere okpomọkụ centrifugation.

2. A na-echekwa ihe nlele ahụ na-ekwesịghị ekwesị ma ọ bụ na-echekwa ihe nlele ahụ ogologo oge.

Nkwanye: Chekwaa ihe nlele na -80 Celsius ma zere ịfriza ugboro ugboro na thawing;gbalịa iji ihe nlele achịkọtara ọhụrụ maka mmịpụta acid nucleic.

3. ezughị ezu sample lysis.

Nkwanye: Biko hụ na ihe nlele na ngwọta na-arụ ọrụ lysis na-agwakọta nke ọma ma tinye ya na ụlọ okpomọkụ (15-25 ° C) maka nkeji 10.

4. mgbakwunye na-ezighi ezi nke eluent.

Aro: Gbaa mbọ hụ na agbakwunyere RNase-Free ddH2O dabara n'etiti akpụkpọ ahụ kọlụm dị ọcha, ma etinyekwala ya na mgbanaka kọlụm dị ọcha.

5. Agbakwunyeghị olu ziri ezi nke ethanol zuru oke na Buffer RW2.

Aro: Biko soro ntuziaka a, tinye nha ziri ezi nke ethanol zuru oke na Buffer RW2 wee gwakọta nke ọma tupu i jiri ngwa ahụ.

6. Mpịakọta ihe atụ na-ekwesịghị ekwesị.

Aro: A na-ahazi 200µl nke nlele maka 500µl nke ihe nchekwa DRL ọ bụla.Nhazi ihe nlere nke ukwuu ga-eme ka mkpụrụ nke mmịpụta nucleic acid dị ala.

7. Mpịakọta elution na-ekwesịghị ekwesị ma ọ bụ elution ezughị ezu.

Nkwanye: Oke eluent nke kọlụm nchacha bụ 30-50μl;Ọ bụrụ na mmetụta elution adịghị afọ ojuju, a na-atụ aro ịgbatị oge n'ime ụlọ okpomọkụ mgbe agbakwunyere ddH2O na-enweghị ọkụ RNase, dị ka 5-10min.

8. Ethanol na-anọgide na kọlụm mgbe ịsachara na Buffer RW2.

Aro: Ọ bụrụ na ethanol na-anọgide mgbe centrifugation na Buffer RW2 maka nkeji 2, a ga-etinye kọlụm n'ime ụlọ okpomọkụ maka nkeji 5 mgbe centrifugation gachara iji wepụ ethanol fọdụrụnụ kpamkpam.

Nucleic acid a na-eme ka ọ dị ọcha na-emebi

Ogo nke nucleic acid a na-eme ka ọ dị ọcha metụtara ichekwa ihe nlele ahụ, mmetọ RNase, ọrụ na ihe ndị ọzọ.Nyocha nke ihe na-akpatakarị:

1. A naghị echekwa ihe nlele ndị anakọtara na oge.

Aro: Ọ bụrụ na ejighị ihe nlele ahụ n'oge nchịkọta, biko chekwaa ya na -80 Celsius na obere okpomọkụ ozugbo.Maka mmịpụta RNA, gbalịa iji ihe nlele anakọtara ọhụrụ.

2. Na-anakọta ihe nlele na ifriizi ma mebie ugboro ugboro.

Aro: Zere oyi na thawing (ọ bụghị ihe karịrị otu ugboro) n'oge nchịkọta na nchekwa nke samples, ma ọ bụghị ya, mkpụrụ nucleic acid ga-ebelata.

3. A na-ewebata RNase n'ime ụlọ ọrụ ma ọ bụ uwe mkpuchi, ihe mkpuchi, wdg.

Nkwanye: A na-eme nnwale mmịpụta RNA kacha mma n'ime ụlọ ọrụ RNA dị iche, yana tebụl ụlọ nyocha kwesịrị ihicha tupu nnwale ahụ.

Yiri gloves na masks ndị enwere ike iwepụ n'oge nnwale ahụ ka ịzenarị mmebi nke RNA kpatara site na iwebata RNase ruo oke.

4. Eji RNase metọọ reagent mgbe eji ya.

Nkwanye: Dochie na Viral DNA/RNA Isolation Kit ọhụrụ maka nnwale ndị metụtara ya.

5. A na-eji RNase metọọ tubes centrifuge na ndụmọdụ pipette eji eme ihe maka RNA.

Aro: Jide n'aka na tubes centrifuge, ndụmọdụ pipette, pipettes, wdg. ejiri maka mmịpụta RNA bụ ihe niile RNase enweghị.

Nucleic acid dị ọcha na-emetụta nnwale ndị dị n'okpuru ala

DNA na RNA sachara site na kọlụm nchacha, ọ bụrụ na ion nnu na protein dị elu, ọ ga-emetụta nnwale ndị dị n'okpuru ala, dịka: nkwalite PCR, ntụgharị ntụgharị, wdg.

1. DNA nke eluted na RNA nwere ion nnu fọdụrụnụ.

Aro: Gbaa mbọ hụ na agbakwunyere olu ziri ezi nke ethanol zuru oke na Buffer RW2, wee saa kọlụm nchacha ugboro abụọ na ọsọ centrifugation akọwapụtara na ntuziaka ọrụ;Mee centrifugation iji belata mmetọ ion nnu.

2. DNA na RNA na-elu elu nwere ethanol residues.

Aro: Mgbe akwadochara ịsa ahụ na Buffer RW2, mee centrifugation tube efu na ọsọ centrifugation na ntuziaka ọrụ;Ọ bụrụ na a ka nwere ihe fọdụrụ na ethanol, ị nwere ike centrifuge tube efu wee tinye ya na ụlọ okpomọkụ maka nkeji 5 iji wepụ ihe fọdụrụ ethanol ruo n'ókè kasị ukwuu.

Akwụkwọ ntuziaka:

Akwụkwọ ntuziaka ngwa mwepu nke DNA na RNA