Nlereanya efu maka China CE/FDA akwadoro Magnetic Beads Usoro DNA / Rna Nucleic Acid Extraction Reagent Kit (Mkpụrụ osisi Magnetik ebuburu ya)
Nkọwa ngwa:
Ọ dịghị mkpa ichegbu onwe gị maka mbibi RNA.Sistemụ a niile enweghị RNase
Wepu DNA nke ọma site na iji kọlụm DNA- Cleaning
Wepu DNA na-agbakwunyeghị DNAse
Mfe-arụ ọrụ niile na-arụcha na ụlọ okpomọkụ
Ngwa ngwa-enwere ike ịrụcha ọrụ n'ime nkeji iri atọ
Safe — ọ dịghị organic reagent eji
Ịdị ọcha dị elu-OD260/280≈1.8-2.1
Anyị na-ọpụrụiche na melite ihe njikwa na QC usoro ka anyị wee na-ejigide egwu onu n'ime ọkụ-asọmpi obere azụmahịa maka Free sample maka China CE / FDA kwadoro Magnetic Beads Method DNA / Rna Nucleic Acid Extraction Reagent Kits (Pre-juru n'ọnụ Magnetic Beads), Iji ghọta reciprocal uru na mba ofesi, anyị na-azụ ahịa ngwa ngwa na mba ofesi, anyị na-azụ ahịa n'ụzọ zuru ezu na-azụ ahịa na mba ọzọ. kacha mma mma mma na ogologo oge imekọ ihe ọnụ.
Anyị nwekwara ọkachamara n'ịkwalite usoro njikwa ihe yana usoro QC ka anyị wee nwee ike ijigide ọmarịcha ọnụ n'ime obere azụmaahịa na-asọ mpi maka.Nucleic acid nke China, Ndị na-emepụta Nucleic Acid China, Ebupụla mmepụta anyị na mba na mpaghara 30 dị ka isi iyi nke mbụ na ọnụ ala dị ala.Anyị na-anabata ndị ahịa si ma n'ụlọ na mba ọzọ ka anyị na ha kparịta ụka.
Nkọwa ngwa ngwa
Nkwadebe 50, 200 Preps
Ngwa a na-eji kọlụm spine na usoro nke ụlọ ọrụ anyị mepụtara, nke nwere ike wepụ ịdị ọcha dị elu na nke dị elu RNA site na anụ ahụ dị iche iche nke anụmanụ na-arụ ọrụ dị elu. Ọ na-enye DNA-Cleaning Column na-arụ ọrụ nke ọma, nke nwere ike ikewapụ na adsorb genomic DNA site na supernatant na anụ ahụ lysate, dị mfe na-azọpụta oge;Kọlụm naanị RNA nwere ike jikọta RNA nke ọma ma nwee ike hazie ya n'otu oge site na iji usoro pụrụ iche Ọtụtụ nlele.
Usoro dum bụ RNase-Free, nke mere na RNA amịpụtara adịghị emebi emebi;Buffer RW1, Buffer RW2 sistemu ịsacha ihe, nke mere na RNA enwetara enweghị protein, DNA, ion, na mmetọ ngwakọta organic.
Ngwa ngwa:
| Ihe nchekwa RL1, ihe nchekwa RL2 |
| Ihe nchekwa RW1, ihe nchekwa RW2 |
| ddH2O enweghị RNase, kọlụm na-ehicha DNA |
| RNA-naanị kọlụm |
Atụmatụ & uru
■ Ọ dịghị mkpa ichegbu onwe gị maka mbibi RNA;Sistemụ niile bụ RNase-Free
∎ Wepu DNA nke ọma site na iji kọlụm na-ehicha DNA
■ Wepu DNA na-agbakwunyeghị DNA
■ A na-arụ ọrụ niile dị mfe na ọnụ ụlọ okpomọkụ
■ Enwere ike ịrụcha ọrụ ngwa ngwa n'ime nkeji iri atọ
■ Nchekwa-ọ dịghị ihe reagent organic achọrọ
■ Ịdị ọcha dị elu -OD260 / 280≈1.8-2.1
Ngwa ngwa
Ọ dabara maka mmịpụta na ịdị ọcha nke mkpokọta RNA site na anụ ahụ dị ọhụrụ ma ọ bụ oyi kpọnwụrụ dị iche iche ma ọ bụ sel emebere.
Ngwa ngwaahịa
∎ Ngwa mgbada ala: njikọ cDNA nke mbụ, RT-PCR, cloning molecular, Northern Blot, wdg.
■ Ihe nlele: anụ ahụ anụmanụ, mkpụrụ ndụ omenala
■ Usoro onunu ogwu: Tissues 10-20mg, Sel (2-5) × 106
∎ Oke kachasị ike ijikọ DNA nke kọlụm nchacha: 80 μg
■ Olu olu: 50-200 μl
Usoro ọrụ
Eserese
Ngwakọta Mkpokọta RNA nke anụmanụ na-agwọ 20mg
Ihe nlele òké ọhụrụ, were 5% RNA 1% agar sachara ọcha
Glycogel electrophoresis
1: Spleen 2: Akụrụ
3: Imeju 4: Obi
Nchekwa na ndụ nchekwa
Enwere ike ịchekwa ngwa ahụ maka ọnwa 24 na okpomọkụ nke ụlọ (15-25 ℃) ma ọ bụ 2-8 ℃ maka ogologo oge.Enwere ike ịchekwa ihe nchekwa RL1 na 4 ℃ maka ọnwa 1 mgbe agbakwunyere β-mercaptoethanol (nhọrọ) .Anyị na-ahụkwa maka imeziwanye njikwa ihe na usoro QC ka anyị wee nwee ike ijigide ihu dị egwu n'ime obere azụmaahịa na-asọ mpi maka Free sample maka China CE/FDA kwadoro Magnetic Beads Method / Rnactive DNA. na-aghọta reiprocal uru, anyị enterprise na-ọtụtụ ebe boosting anyị tactics nke ijikọ ụwa ọnụ na okwu nke nkwurịta okwu na mba ndị na-azụ ahịa, ngwa ngwa nnyefe, kasị mma mma na ogologo oge imekọ ihe ọnụ.
Nlereanya efu makaNucleic acid nke China, Ndị na-emepụta Nucleic Acid China, Ebupụla mmepụta anyị na mba na mpaghara 30 dị ka isi iyi nke mbụ na ọnụ ala dị ala.Anyị na-anabata ndị ahịa si ma n'ụlọ na mba ọzọ ka anyị na ha kparịta ụka.
