Ihe nlele efu maka China High Sensitivity DNA/Rna Otu nzọụkwụ nyocha Rt-Qpcr Kit V2
Nkọwa ngwa:
◮Ngwa otu nzọụkwụ na-eme ntụgharị ntụgharị na qPCR mmeghachi omume abụọ n'otu tube, naanị ịkwesịrị ịgbakwunye RNA template, kpọmkwem PCR primers na RNase-Free ddH.2O.
◮Ngwa ahụ nwere ike nyochaa ngwa ngwa RNA nke nje virus ma ọ bụ traktị RNA.
◮Ngwa ahụ na-eji ihe ntụgharị ntụgharị pụrụ iche nke Foregene na Foregene HotStar Taq DNA Polymerase jikọtara ya na sistemu mmeghachi omume pụrụ iche iji melite arụmọrụ nkwalite na nkọwapụta mmeghachi omume ahụ nke ọma.
◮Usoro mmeghachi omume kachasị mma na-eme ka mmeghachi omume ahụ nwee mmetụta nchọpụta dị elu, nkwụsi ike okpomọkụ siri ike, na ndidi ka mma.
◮RT-qPCR dị mfeTM(Otu Nzọụkwụ) -SYBR Green I kit na-abịa na ROX n'ime ntụ ntụ ntụ, nke enwere ike iji wepụ ihe mgbaàmà na njehie mgbaàmà n'etiti olulu mmiri, nke dị mma maka ndị ahịa iji na ụdị dị iche iche nke akụrụngwa PCR.
Anyị kwenyere na: Innovation bụ mkpụrụ obi anyị na mmụọ anyị.Ịdị mma bụ ndụ anyị.Mkpa ndị ahịa nwere bụ Chineke anyị maka Free sample maka China High Sensitivity DNA/Rna One-Step Probe Rt-QpcrKit V2, N'ime atụmatụ anyị, anyị enweelarị ọtụtụ ụlọ ahịa na China na azịza anyị enwetala otuto sitere n'aka ndị na-azụ ahịa n'ụwa niile.Nabata ndị na-azụ ahịa ọhụrụ na ihe mgbe ochie ka ha kpọtụrụ anyị na mmekọrịta azụmahịa na-adịte aka ogologo oge.
Anyị kwenyere na: Innovation bụ mkpụrụ obi anyị na mmụọ anyị.Ịdị mma bụ ndụ anyị.Consumer mkpa inwe bụ Chineke anyị makaChina Taq DNA Polymerase, Qpcr, Ọrụ siri ike iji nọgide na-enwe ọganihu, ihe ọhụrụ na ụlọ ọrụ, na-eme mgbalị ọ bụla na ụlọ ọrụ mbụ.Anyị na-agbalị ike anyị niile iji wuo usoro nchịkwa sayensị, ịmụta ọtụtụ ihe ọmụma ọkachamara, ịmepụta ngwá ọrụ mmepụta ihe na usoro mmepụta ihe, ịmepụta ngwaahịa mbụ na-akpọ ngwaahịa, ọnụahịa ezi uche dị na ya, ọrụ dị elu, nnyefe ngwa ngwa, iji nye gị ịmepụta uru ọhụrụ.
Nkọwapụta
50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns
Ngwa ngwa
| Akụkụ I | Ihe nchekwa P1 |
| Ihe nchekwa P2 | |
| 6 × DNA na-ebufe ihe nchekwa | |
| Akụkụ II | 2× Akwukwo PCR MfeTMNgwakọta (UNG) |
Atụmatụ & uru
∎ Ọ dịghị ọcha DNA na-ewe oge yana ọnụ
■ Ihe dị obere
∎ Mfe - enwere ike idobe ihe nlele ahụ na mmeghachi omume PCR ma ọ bụ mmeghachi omume lysis na-egbutughị ma ọ bụ egweri
∎ 2 × Mix, na-ebelata mperi mperi ihe nlele na oge nkwadebe usoro mmeghachi omume
■ Enwere ike ịmechaa nkwadebe ngwa ngwa n'ime nkeji 10, enwere ike mechaa mmeghachi omume PCR n'ime opekata mpe nkeji iri ise.
∎ Enwere ike mechaa mmeghachi omume nnabata dị elu na efere PCR 96 nke ọma
Ngwa ngwa
Ngwa: njirimara gbanwetụrụ na mkpụrụ ndụ ihe nketa, genotyping, wdg.
Ihe atụ: akwukwo osisi
Usoro onunu ogwu: dayameta 2-3mm (usoro ozugbo), dayameta 5-7mm (usoro lysis)
Oke nchọpụta: iberibe ebumnuche ≤1kb
Usoro ọrụ
Electropherogram
Izu abụọ nke PCR Mix nchoputa nchọpụta n'okpuru ọnọdụ nke PCR amplification ngwaahịa contamination.Anyị kwenyere na: Innovation bụ mkpụrụ obi anyị na mmụọ.Ịdị mma bụ ndụ anyị.Consumer need to have is our God for Free sample for China High Sensitivity DNA/Rna One-Step Probe Rt-Qpcr Kit V2, Inside our initiatives, we have already have a lot of shops in China and our solutions have winning praise from purchasers all the world.Nabata ndị na-azụ ahịa ọhụrụ na ihe mgbe ochie ka ha kpọtụrụ anyị na mmekọrịta azụmahịa na-adịte aka ogologo oge.
Nlereanya efu makaChina Taq DNA Polymerase, Qpcr, Ịrụ ọrụ siri ike ịnọgide na-enwe ọganihu, ihe ọhụrụ na ụlọ ọrụ, na-eme mgbalị ọ bụla na ụlọ ọrụ mbụ.Anyị na-agbalị ike anyị niile iji wuo usoro nchịkwa sayensị, ịmụta ọtụtụ ihe ọmụma ọkachamara, ịmepụta ngwá ọrụ mmepụta ihe na usoro mmepụta ihe, ịmepụta ngwaahịa mbụ na-akpọ ngwaahịa, ọnụahịa ezi uche dị na ya, ọrụ dị elu, nnyefe ngwa ngwa, iji nye gị ịmepụta uru ọhụrụ.
