Ogo dị elu maka China 96 Well Plate Test DNA/Rna Nucleic Acid Extract Isolation Reagent Kit nwere usoro bead magnetik maka nchọpụta PCR.
Ebumnuche anyị ga-abụ imezu ndị ahịa anyị site n'inye enyemaka ọla edo, ọnụahịa dị egwu na ịdị elu dị elu maka ogo dị elu maka China 96 Well Plate Test DNA/Rna Nucleic Acid Extract Isolation Reagent Kit with Magnetic Bead Method for PCR Detection, Ugbu a, anyị nwere otu ọkachamara maka ahia mba ụwa.Anyị ga-edozi nsogbu ị zutere.Anyị nwere ike weta ngwongwo ị chọrọ.Jide n'aka na ị na-eche na-efu efu ịkpọtụrụ anyị.
Ebumnuche anyị ga-abụ imezu ndị na-azụ ahịa anyị site n'inye enyemaka ọla edo, ọnụahịa dị egwu na ịdị elu dị elu makaChina DNA Rna iche, Mwepu Nucleic Acid Viral, Ngwa ngwa na ọkachamara mgbe-ịre ahịa na-eweta site na ndị ọkachamara anyị nwere obi ụtọ ndị na-azụ anyị.A ga-ezigara gị ozi zuru ezu na parampat sitere na ngwa ahịa maka nkwenye zuru oke ọ bụla.Enwere ike ibunye ihe nlele efu na ụlọ ọrụ lelee ụlọ ọrụ anyị.n Morocco maka mkparita uka na-anabata mgbe niile.Na-atụ anya ịnweta ajụjụ pịnye gị wee wulite mmekọrịta nkwado ogologo oge.
Nkọwa ngwa ngwa
Nkwadebe 50, 200 Preps
Ngwa a na-eji kọlụm spine na usoro nke ụlọ ọrụ anyị mepụtara, nke nwere ike wepụ ịdị ọcha dị elu na nke dị elu RNA site na anụ ahụ dị iche iche nke anụmanụ na-arụ ọrụ dị elu. Ọ na-enye DNA-Cleaning Column na-arụ ọrụ nke ọma, nke nwere ike ikewapụ na adsorb genomic DNA site na supernatant na anụ ahụ lysate, dị mfe na-azọpụta oge;Kọlụm naanị RNA nwere ike jikọta RNA nke ọma ma nwee ike hazie ya n'otu oge site na iji usoro pụrụ iche Ọtụtụ nlele.
Usoro dum bụ RNase-Free, nke mere na RNA amịpụtara adịghị emebi emebi;Buffer RW1, Buffer RW2 sistemu ịsacha ihe, nke mere na RNA enwetara enweghị protein, DNA, ion, na mmetọ ngwakọta organic.
Ngwa ngwa:
| Ihe nchekwa RL1, ihe nchekwa RL2 |
| Ihe nchekwa RW1, ihe nchekwa RW2 |
| ddH2O enweghị RNase, kọlụm na-ehicha DNA |
| RNA-naanị kọlụm |
Atụmatụ & uru
■ Ọ dịghị mkpa ichegbu onwe gị maka mbibi RNA;Sistemụ niile bụ RNase-Free
∎ Wepu DNA nke ọma site na iji kọlụm na-ehicha DNA
■ Wepu DNA na-agbakwunyeghị DNA
■ A na-arụ ọrụ niile dị mfe na ọnụ ụlọ okpomọkụ
■ Enwere ike ịrụcha ọrụ ngwa ngwa n'ime nkeji iri atọ
■ Nchekwa-ọ dịghị ihe reagent organic achọrọ
■ Ịdị ọcha dị elu -OD260 / 280≈1.8-2.1
Ngwa ngwa
Ọ dabara maka mmịpụta na ịdị ọcha nke mkpokọta RNA site na anụ ahụ dị ọhụrụ ma ọ bụ oyi kpọnwụrụ dị iche iche ma ọ bụ sel emebere.
Ngwa ngwaahịa
∎ Ngwa mgbada ala: njikọ cDNA nke mbụ, RT-PCR, cloning molecular, Northern Blot, wdg.
■ Ihe nlele: anụ ahụ anụmanụ, mkpụrụ ndụ omenala
■ Usoro onunu ogwu: Tissues 10-20mg, Sel (2-5) × 106
∎ Oke kachasị ike ijikọ DNA nke kọlụm nchacha: 80 μg
■ Olu olu: 50-200 μl
Usoro ọrụ
Eserese
Ngwakọta Mkpokọta RNA nke anụmanụ na-agwọ 20mg
Ihe nlele òké ọhụrụ, were 5% RNA 1% agar sachara ọcha
Glycogel electrophoresis
1: Spleen 2: Akụrụ
3: Imeju 4: Obi
Nchekwa na ndụ nchekwa
Enwere ike ịchekwa ngwa ahụ maka ọnwa 24 na okpomọkụ nke ụlọ (15-25 ℃) ma ọ bụ 2-8 ℃ maka ogologo oge.Enwere ike ịchekwa ihe nchekwa RL1 na 4 ℃ maka otu ọnwa ka ịgbakwunye β-mercaptoethanol (nhọrọ).
Ebumnuche anyị ga-abụ imezu ndị ahịa anyị site n'inye enyemaka ọla edo, ọnụahịa dị egwu na ịdị elu dị elu maka ogo dị elu maka China 96 Well Plate Test DNA/Rna Nucleic Acid Extract Isolation Reagent Kit with Magnetic Bead Method for PCR Detection, Ugbu a, anyị nwere otu ọkachamara maka ahia mba ụwa.Anyị ga-edozi nsogbu ị zutere.Anyị nwere ike weta ngwongwo ị chọrọ.Jide n'aka na ị na-eche na-efu efu ịkpọtụrụ anyị.
Ogo dị elu makaChina DNA Rna iche, Mwepu Nucleic Acid Viral, Ngwa ngwa na ọkachamara mgbe-ịre ahịa na-eweta site na ndị ọkachamara anyị nwere obi ụtọ ndị na-azụ anyị.A ga-ezigara gị ozi zuru ezu na parampat sitere na ngwa ahịa maka nkwenye zuru oke ọ bụla.Enwere ike ibunye ihe nlele efu na ụlọ ọrụ lelee ụlọ ọrụ anyị.n Morocco maka mkparita uka na-anabata mgbe niile.Na-atụ anya ịnweta ajụjụ pịnye gị wee wulite mmekọrịta nkwado ogologo oge.
