Kedu nke nwere àgwà dị mma na nke na-aga n'ihu na mmasị ndị ahịa, ụlọ ọrụ anyị na-eme ka ihe anyị dịkwuo mma iji mejuo ọchịchọ nke ndị ahịa na n'ihu na-elekwasị anya na nchekwa, ntụkwasị obi, mkpa gburugburu ebe obibi, na ihe ọhụrụ nke Onye nrụpụta maka China Magnetic Beads Nucleic Acid PCR Extraction Kit na CE maka Poct PCR Machine, Ezi olileanya na anyị na-eto eto ọnụ na gburugburu anyị atụmanya niile.
Nke nwere àgwà dị mma na nke na-aga n'ihu na mmasị ndị ahịa, ụlọ ọrụ anyị na-eme ka ihe anyị dịkwuo mma iji mejuo ọchịchọ nke ndị ahịa ma na-elekwasị anya na nchekwa, ntụkwasị obi, ihe ndị chọrọ gburugburu ebe obibi, na mmepụta ọhụrụ nkeNgwa ihe mmịpụta DNA nke China, Ngwa Nwepụta Acid Nucleic, Anyị na-agbaso ndị ahịa 1st, top quality 1st, na-aga n'ihu na-emeziwanye, uru ibe na-emeri ụkpụrụ.Mgbe anyị na ndị ahịa na-emekọ ihe ọnụ, anyị na-ebuga ndị na-azụ ahịa nwere ọrụ kachasị elu.N'iji onye na-azụ ahịa Zimbabwe guzobe ezigbo mmekọrịta azụmahịa n'ime azụmahịa ahụ, anyị enwetala akara na aha nke anyị.N'otu oge ahụ, jiri obi dum nabata atụmanya ọhụrụ na ochie na ụlọ ọrụ anyị ịga na kparịta ụka obere azụmahịa.

Nkọwa

Ngwa a na-eji sistemu nchekwa lysis pụrụ iche nke nwere ike hapụ RNA ngwa ngwa site na nlele cell emebere maka mmeghachi omume RT-qPCR, si otú a na-ewepụ usoro ịdị ọcha nke RNA na-ewe ma na-arụsi ọrụ ike.Enwere ike nweta ndebiri RNA n'ime naanị nkeji 7.Ngwakọta 5 × Direct RT Mix na 2 × Direct qPCR Mix-SYBR reagents nke ngwa ahụ na-enye nwere ike nweta nsonaazụ PCR ozugbo na nke ọma.

5 × Direct RT Mix na 2 × Direct qPCR Mix-SYBR nwere nnabata inhibitor siri ike, enwere ike iji lysate nke ihe nlele ahụ dị ka ndebiri maka RT-qPCR ozugbo.Ngwa a nwere RNA pụrụ iche Foregene reverse transcriptase, yana Hot D-Taq DNA polymerase, dNTPs, MgCl.₂, ihe nchekwa mmeghachi omume, PCR optimizer na stabilizer.

Nkọwapụta

200 × 20μl Rxns, 1000 × 20μl Rxns

Ngwa ngwa

Atụmatụ & uru

∎ Dị mfe ma dị irè: site na teknụzụ Cell Direct RT, enwere ike nweta ihe nlele RNA n'ime naanị nkeji asaa.

■ Ọchịchọ nlele dị ntakịrị, ebe ọ bụ na enwere ike ịnwale mkpụrụ ndụ 10.

∎ Mgbakwunye dị elu: ọ nwere ike ịchọpụta RNA ngwa ngwa n'ime sel ndị emebere na 384, 96, 24, 12, 6-well plates.

∎ DNA Eraser nwere ike wepụ ngwa ngwa genomes ewepụtara, belata mmetụta dị na nsonaazụ nnwale na-esote.

∎ Sistemu RT na qPCR emezigharịrị na-eme ka ntụgharị ntụgharị RT-PCR nzọụkwụ abụọ rụọ ọrụ nke ọma yana PCR akọwapụtara nke ọma, ma na-eguzogide ndị na-egbochi mmeghachi omume RT-qPCR.

Ngwa ngwa

Oke ngwa: mkpụrụ ndụ omenala.

- RNA wepụtara site na nlele lysis: naanị ọdabara na ndebiri RT-qPCR nke ngwa a.

- Enwere ike iji ngwa ahụ maka ebumnuche ndị a: nyocha okwu mkpụrụ ndụ ihe nketa, nkwenye nke mmetụta ịgbachitere mkpụrụ ndụ ihe nke siRNA, nyocha ọgwụ, wdg.

Eserese

Nchekwa na Shelf ndụ

A ga-echekwa akụkụ nke mbụ nke ngwa a na 4 ℃;Ekwesịrị ịchekwa akụkụ II na -20 ℃.

Ekwesịrị ịchekwa Foregene Protease Plus II na 4 ℃, echekwala na -20 ℃.

Ekwesịrị ịchekwa Reagent 2 × Direct qPCR Mix-SYBR na -20℃ n'ọchịchịrị;ma ọ bụrụ na-eji ugboro ugboro, ọ nwekwara ike ga-echekwara na 4 ℃ maka obere oge nchekwa (jiri elu n'ime 10 ụbọchị) .Which nwere a nti na-aga n'ihu àgwà ka ndị ahịa mmasị, anyị Enterprise anọgide mma anyị item magburu onwe ya imeju ọchịchọ nke ndị ahịa na n'ihu na-elekwasị anya na nchekwa, ntụkwasị obi, gburugburu ebe obibi chọrọ, na ọhụrụ nke Manufacturer maka China Magnetic Beads na PCR Ngwunye PCR N'ihi na anyị nwere ike na-eto eto PCR N'ihi na PCR na-eto eto n'ike n'ike. yana atụmanya anyị na gburugburu ebe obibi.
Onye nrụpụta makaNgwa ihe mmịpụta DNA nke China, Ngwa Nwepụta Acid Nucleic, Anyị na-agbaso ndị ahịa 1st, top quality 1st, na-aga n'ihu na-emeziwanye, uru ibe na-emeri ụkpụrụ.Mgbe anyị na ndị ahịa na-emekọ ihe ọnụ, anyị na-ebuga ndị na-azụ ahịa nwere ọrụ kachasị elu.N'iji onye na-azụ ahịa Zimbabwe guzobe ezigbo mmekọrịta azụmahịa n'ime azụmahịa ahụ, anyị enwetala akara na aha nke anyị.N'otu oge ahụ, jiri obi dum nabata atụmanya ọhụrụ na ochie na ụlọ ọrụ anyị ịga na kparịta ụka obere azụmahịa.