Onye nrụpụta China CE Asambodo Real-Time PCR Reagent Kit maka Nje Virus Ọhụrụ 2019 (O, N gene)
Nkọwa ngwa:
Nke a bụ kpọmkwem PCR:Enweghị mkpa maka ngwa mkpochapụ acid nucleic acid na Sistemu, nke emechara 96 n'ime elekere 1 ma ọ bụ karịa.
◮ Ngwa akụrụngwa dị ala yana ojiji nke ukwuu na-agbanwe agbanwe:Naanị sistemụ PCR Real-Time chọrọ.
LoD na mmetụta dị elu:Nchọpụta nchọta nwere ike iru ọkwa kacha ala dị ka 500 nnomi/ml.
Anyị ngwọta na-ukwuu ghọtara na a pụrụ ịdabere na site ahịa na ga-emezu nọgidere na-agbanwe ego na-elekọta mmadụ chọrọ maka emeputa nke China CE Certificate Real-Time PCR Reagent Kit maka 2019 New Virus (O, N gene), Anyị na-achụ nta n'ihu na-ewu nti na bara uru njikọ na ulo oru gburugburu ụwa.Anyị ji obi ụtọ na-anabata gị ka ị kpọọ anyị ka anyị malite mkparịta ụka maka otu anyị nwere ike isi webata nke a ngwa ngwa.
Anyị ngwọta na-ukwuu ghọtara na a pụrụ ịdabere na site ahịa na ga-emezu nọgidere na-agbanwe ego na-elekọta mmadụ chọrọ makaNgwa PCR nke China, Ngwa nchọpụta PC, Anyị kwenyere na ịmepụta mmekọrịta ndị ahịa dị mma na mmekọrịta dị mma maka azụmahịa.Mmekọrịta dị nso na ndị ahịa anyị enyerela anyị aka ịmepụta ụdọ ọkọnọ siri ike ma nweta uru.Ngwa ahịa anyị enwetala anyị nnabata zuru oke yana afọ ojuju nke ndị ahịa anyị ji ama ama n'ụwa niile.
Nkọwapụta
48 Rxns, 96 Rxns
| Mba. | Akụkụ | Ọnụ ego | Isi ihe | |||
| 48 Rxn | 96 Rxn | |||||
| 1 | Ihe ntọhapụ nke Nucleic acid | 1.4ml / tube | 2 ọkpọkọ | 5.3 ml / karama | 1 karama | Surfactant |
| 2 | ihe nchekwa RNA | 27 μl / tube | 1 tube | 53 μl / tube | 1 tube | Ihe mgbochi RNase |
| 3 | Ngwọta mmeghachi omume SARS-CoV-2 | 800 μl / tube | 1 tube | 1600 μl / tube | 1 tube | Ndabere, nyocha, ihe nchekwa mmeghachi omume, dNTP |
| 4 | Ngwakọta enzyme SARS-CoV-2 | 80 μl / tube | 1 tube | 160 μl / tube | 1 tube | Mmalite Taq Enzyme dị ọkụ, M-MLV enzyme |
| 5 | SARS-CoV-2 ezigbo njikwa | 100 μl / tube | 1 tube | 100 μl / tube | 1 tube | Plasmid recombinant nwere iberibe ebumnuche, RNA |
| 6 | SARS-CoV-2 njikwa adịghị mma | 1200 μl / tube | 1 tube | 1200 μl / tube | 1 tube | Ihe nchekwa TE |
Atụmatụ & uru
Ngwa ngwa na mfe.
Zere mfu nucleic acid.
Oke nchọpụta dị ala na nghọta dị elu.
Achọrọ akụrụngwa dị ala yana iji mgbanwe dị ukwuu.
Nchọpụta nchọpụta nwere ike iru ọkwa kacha ala dị ka 500 mbipụta / ml).
| GCE/ml | Ct uru | |
| N gene | ORF1ab mkpụrụ ndụ ihe nketa | |
| 2.00E+08 | 16.43 | 15.16 |
| 2.00E+07 | 19.48 | 18.58 |
| 2.00E+06 | 22.71 | 21.86 |
| 2.00E+05 | 25.95 | 25.33 |
| 2.00E+04 | 29.30 | 28.49 |
| 2.00E+03 | 32.37 | 31.37 |
| 2.00E+02 | 35.32 | 34.47 |
| 2.00E+01 | 38.29 | 38.27 |
| 2.00E+00 | N/A | N/A |
| 2.00E-01 | N/A | N/A |
| NC | N/A | N/A |
Usoro mmụba nke ahịrị
Otu esi eji
1. Ngwa eji eme ihe
2. Usoro nchọpụta
Nhazi ihe nlele: Naopharyngeal ma ọ bụ oropharyngeal swab sample (naanị ọrụ nzọụkwụ 1) (Chere 10 mins)
PCR amplificaton (<= 55min)
Usoro ọrụ:
Nchekwa
mechie site na ìhè ma chekwaa na -20 ± 5 ℃;
Ndụ nchekwa:1 yearOur ngwọta na-ukwuu ghọtara na ntụkwasị obi site ahịa na ga-emezu nọgidere na-agbanwe ego na-elekọta mmadụ chọrọ maka emeputa nke China CE Certificate Real-Time PCR Reagent Kit maka 2019 New Virus (O, N gene), Anyị na-achụ nta n'ihu na-ewu nti na bara uru njikọ na ulo oru gburugburu ụwa.Anyị ji obi ụtọ na-anabata gị ka ị kpọọ anyị ka anyị malite mkparịta ụka maka otu anyị nwere ike isi webata nke a ngwa ngwa.
Onye nrụpụta nkeNgwa PCR nke China, PCR Detection Kit, Anyị kwenyere na ịmepụta mmekọrịta ndị ahịa dị mma na mmekọrịta dị mma maka azụmahịa.Mmekọrịta dị nso na ndị ahịa anyị enyerela anyị aka ịmepụta ụdọ ọkọnọ siri ike ma nweta uru.Ngwa ahịa anyị enwetala anyị nnabata zuru oke yana afọ ojuju nke ndị ahịa anyị ji ama ama n'ụwa niile.
