Ọrụ anyị kwesịrị ịbụ ijere ndị ọrụ njedebe anyị na ndị na-azụ ahịa ozi ngwaahịa kacha mma yana ngwaahịa dijitalụ nwere ike ịsọ mpi makaPCR Reagent, Anyị ji ezi obi na-anabata ndị abụọ esenidụt na anụ ụlọ azụmahịa Enterprise mmekọ, na-atụ anya na-arụ ọrụ na gị na gburugburu ogologo okwu!
Ọrụ anyị kwesịrị ịbụ ijere ndị ọrụ njedebe anyị na ndị na-azụ ahịa ozi ngwaahịa kacha mma yana ngwaahịa dijitalụ nwere ike ịsọ mpi makaPCR Reagent, Na fọrọ nke nta 30 afọ ahụmahụ na azụmahịa, anyị na-enwe obi ike na elu ọrụ, àgwà na nnyefe.Anyị ji obi ụtọ na-anabata ndị ahịa si n'akụkụ ụwa niile ka ha na ụlọ ọrụ anyị kwadoro maka mmepe nkịtị.

Nkọwapụta

5ml, 10ml, 40ml

Ngwa ngwa

Atụmatụ & uru

■ Ikwesị ntụkwasị obi dị elu: 6 ugboro nke enzyme Taq nkịtị;

■ Ọsọ nkwuwa okwu ngwa ngwa: ọsọ nkwalite bụ 5-10sec/kb, nke bụ 6-12 ugboro nke enzyme Taq nkịtị;

∎ Mgbanwe nke ndebiri: Ọ nwere ike ịkwalite ndebiri DNA dị mgbagwoju anya dị elu nke ọmaGC ọdịnaya;

■ Ịrụ ọrụ nkwalite dị elu: okirikiri mmụba dị ala karịa enzyme Taq nkịtị;

■ Nkwekọrịta gburugburu ebe obibi siri ike: etinye ya na 37 ° C maka otu izu, na-ejigide ihe karịrị 90% ọrụ;

■ Ọ nwere 5′ → 3′ DNA polymerase ọrụ na 5′→3′ exonuclease ọrụ, na-enweghị 3′ →5′ exonuclease ọrụ.

■ A na-ejikọta ngwa ahụ na Electrophoresis Loading Buffer, mgbe PCR gwụchara, enwere ike ibunye ihe nlele ahụ ozugbo maka electrophoresis;ọrụ ahụ dị mfe ma dị mfe.

Ngwa ngwa

Mmụba PCR nke iberibe DNA, akara DNA, usoro DNA, PCR tinyere A ọdụ

Nchekwa na Shelf ndụ

Enwere ike ịchekwa ya ogologo oge na -20 Celsius;maka ugboro ugboro, ọ nwere ike ịchekwa na 4 ° C maka obere oge. The 2 × Real PCR EasyTM Mix-SYBR nyere site Real Time PCR EasyTM-SYBR Green m kit bụ a ọhụrụ premix usoro na-eji SYBR Green m maka Real Time PCR amplification mmeghachi omume, nke nwere ike ukwuu melite ngwaahịa specificity na mmeghachi omume uche .A na-enye ROX ka ọ bụrụ ihe eji agba agba n'ime.The fluorescence ike nke kit a bụ 3-5 ugboro nke ngwaahịa ndị yiri ya, nke nwere ike na-enwe mmetụta karịa na kensinammuo na-egosipụta ịta nke lekwasịrị template DNA.
2 × Real PCR EasyTM Mix-SYBR nwere mmalite mmalite Taq DNA Polymerase pụrụ iche nke Foregene.N'iji ya na enzymes Taq nkịtị, ọ nwere uru dị na nrụpụta nkwalite dị elu, ikike nkwalite siri ike yana ọnụ ọgụgụ na-adaghị adaba.Ọ nwere ike belata mmụba na-abụghị nke akọwapụtara ma melite izi ezi nke PCR.