Nka pụrụ iche maka ire ere ọkụ China 2022 ngwa ngwa PCR ngwa ngwa maka Nje Virus Monkeypox
Nkọwa ngwa:
Ngwakọta dị mfe-2X PCR iji belata njehie nnwale na oge ọrụ
Ihe nchekwa akọwapụtara nke ọma na mmalite Taq enzyme nwere ike igbochi nkwalite na-abụghị nke akọwapụtara yana nguzobe primer dimer.
Mmetụta dị elu-nwere ike ịchọpụta obere ndebiri nke ndebiri
Ọdịiche dị mma—dakọtara na ngwa PCR kacha adị adị
Anyị na-arapara na tiori nke "àgwà mbụ, ụlọ ọrụ mbụ, ọganihu na-aga n'ihu na ihe ọhụrụ iji mejuo ndị ahịa" maka njikwa na "nkwụsị efu, mkpesa efu" dị ka ebumnobi dị mma.Iji zuo oke nke onye na-eweta anyị, anyị na-ebuga ihe ndị ahụ na ihe dị mma dị mma na uru bara uru maka Special Design for 2022 China Hot Sale Rapid Real Time PCR Kit maka Monkeypox Virus, Na-emetụta ụlọ ahịa ngwa ngwa nke nri na ihe ọṅụṅụ na-aba n'anya n'ụwa niile , We're wanting forward to operating with partners/ clients for making good results together.
Anyị na-arapara na tiori nke "àgwà mbụ, ụlọ ọrụ mbụ, ọganihu na-aga n'ihu na ihe ọhụrụ iji mejuo ndị ahịa" maka njikwa na "nkwụsị efu, mkpesa efu" dị ka ebumnobi dị mma.Iji mee ka onye na-eweta anyị zuo oke, anyị na-ebufe ihe ndị ahụ yana ezigbo mma dị mma na uru bara uru maka yaNgwa PCR nke anwụ anwụ China na ngwa ule, Site na teknụzụ dị ka isi, mepụta ma mepụta ngwaahịa dị elu dị ka mkpa dị iche iche nke ahịa si dị.Site na echiche a, ụlọ ọrụ ahụ ga-aga n'ihu na-emepụta ngwaahịa na ụkpụrụ dị elu ma na-aga n'ihu na-emezi ngwaahịa na ngwọta, ma nye ọtụtụ ndị ahịa ngwaahịa na ngwọta na ọrụ kacha mma!
Nkọwa Kit
2 × Real PCR dị mfeTMNgwakọta-SYBR nke Real Time PCR dị mfeTM-SYBR Green I kit bụ usoro premix ọhụrụ nke na-eji SYBR Green I maka mmeghachi omume nkwalite PCR oge, nke nwere ike melite nkọwapụta ngwaahịa na mmeghachi omume mmeghachi omume.A na-enye ROX ka ọ bụrụ ihe eji agba agba n'ime.The fluorescence ike nke kit a bụ 3-5 ugboro nke ngwaahịa ndị yiri ya, nke nwere ike na-enwe mmetụta karịa na kensinammuo na-egosipụta ịta nke lekwasịrị template DNA.
2 × Ezigbo PCR dị mfeTMMix-SYBR nwere mmalite mmalite Taq DNA Polymerase pụrụ iche nke Foregene.N'iji ya na enzymes Taq nkịtị, ọ nwere uru dị na nrụpụta nkwalite dị elu, ikike nkwalite siri ike yana ọnụ ọgụgụ na-adaghị adaba.Ọ nwere ike belata mmụba na-abụghị nke akọwapụtara ma melite izi ezi nke PCR.
Nkọwapụta
| Ezigbo oge PCR Easyᵀᴹ-SYBR Green I Kit | ||||
| Ngwakọta ngwa (usoro 20μl) | QP-01011 | QP-01012 | QP-01013 | QP-01014 |
| 200T | 500T | 1000T | 2000T | |
| 2 × Ezigbo PCR dị mfeTMNgwakọta-SYBR | 1 ml × 2 | 1.7 ml × 3 | 1.7 ml × 6 | 1.7 ml × 12 |
| 50× ROX ntụaka agbaji | 400 μl | 1 ml | 1 ml × 2 | 1 ml × 4 |
| ddH na-efu DNAase2O | 1.7 ml | 1.7 ml × 2 | 10 ml | 20 ml |
| Ntuziaka | 1 | 1 | 1 | 1 |
Atụmatụ & uru
∎ Mfe—2X PCR Ngwakọta iji belata njehie nnwale na oge ọrụ
∎ Specific—nkwadebe nke kachasị na enzyme Taq mmalite na-ekpo ọkụ nwere ike igbochi nkwalite na-abụghị nke akọwapụtara na nhazi ọkwa mbụ.
∎ Mmetụta dị elu-nwere ike ịchọpụta obere ndebiri nke ndebiri
∎ Ọdịiche dị mma—dakọtara na ọtụtụ ngwa PCR ọnụ ọgụgụ dị adị
Ngwa ngwa
qPCR nyocha
Usoro ọrụ
Eserese
Nchekwa na ndụ nchekwa
Ekwesịrị ịchekwa ngwa a n'ebe dịpụrụ adịpụ na ọkụ ma chekwaa ya na -20 ℃.Ọ bụrụ na ejiri ya ugboro ugboro, enwere ike ịchekwa ya na 4 ℃ maka obere oge (ụbọchị iri).
Anyị na-arapara na tiori nke "àgwà mbụ, ụlọ ọrụ mbụ, ọganihu na-aga n'ihu na ihe ọhụrụ iji mejuo ndị ahịa" maka njikwa na "nkwụsị efu, mkpesa efu" dị ka ebumnobi dị mma.Iji zuo oke nke onye na-eweta anyị, anyị na-ebuga ihe ndị ahụ na ihe dị mma dị mma na uru bara uru maka Special Design for 2022 China Hot Sale Rapid Real Time PCR Kit maka Monkeypox Virus, Na-emetụta ụlọ ahịa ngwa ngwa nke nri na ihe ọṅụṅụ na-aba n'anya n'ụwa niile , We're wanting forward to operating with partners/ clients for making good results together.
Pụrụ iche imewe makaNgwa PCR nke anwụ anwụ China na ngwa ule, Site na teknụzụ dị ka isi, mepụta ma mepụta ngwaahịa dị elu dị ka mkpa dị iche iche nke ahịa si dị.Site na echiche a, ụlọ ọrụ ahụ ga-aga n'ihu na-emepụta ngwaahịa na ụkpụrụ dị elu ma na-aga n'ihu na-emezi ngwaahịa na ngwọta, ma nye ọtụtụ ndị ahịa ngwaahịa na ngwọta na ọrụ kacha mma!
