N'ogbe OEM China Laboratory Nucleic Acid Extractor Extractor Reagent Kit maka nchọpụta PCR
Ọ dị mma na-abịa 1st;ọrụ bụ nke mbụ;obere azụmahịa bụ imekọ ihe ọnụ” bụ anyị nzukọ nkà ihe ọmụma nke a na mgbe niile hụrụ na achụso site anyị ụlọ ọrụ maka N'ogbe OEM China Laboratory Nucleic Acid Extractor Extraction Reagent Kit maka PCR nchọpụta , Ezi obi olileanya na anyị na-eto eto ọnụ na ndị ahịa anyị n'ụwa nile.
Ọ dị mma na-abịa 1st;ọrụ bụ nke mbụ;obere azụmahịa bụ imekọ ihe ọnụ" bụ nkà ihe ọmụma nzukọ anyị nke a na-ahụ mgbe niile ma na-achụso ụlọ ọrụ anyị makaChina Nucleic Acid Extractor, Nucleic Acid mmịpụta, Akụrụngwa siri ike bụ mkpa inwe nzukọ ọ bụla.A na-akwado anyị site na akụrụngwa akụrụngwa siri ike nke na-enyere anyị aka imepụta, chekwaa, lelee mma na izipu ihe ngwọta anyị n'ụwa niile.Iji nọgide na-arụ ọrụ nke ọma, ugbu a, anyị ekewaala akụrụngwa anyị n'ọtụtụ ngalaba.Ngalaba ndị a niile na-arụ ọrụ na ngwa ọhụrụ, igwe na akụrụngwa emezigharịrị.N'ihi nke a, anyị nwere ike ịrụpụta nnukwu mmepụta na-emebighị ịdịmma ya.
Nkọwapụta
48 Rxns, 96 Rxns
| Mba. | Akụkụ | Ọnụ ego | Isi ihe | |||
| 48 Rxn | 96 Rxn | |||||
| 1 | Ihe ntọhapụ nke Nucleic acid | 1.4ml / tube | 2 ọkpọkọ | 5.3 ml / karama | 1 karama | Surfactant |
| 2 | ihe nchekwa RNA | 27 μl / tube | 1 tube | 53 μl / tube | 1 tube | Ihe mgbochi RNase |
| 3 | Ngwọta mmeghachi omume SARS-CoV-2 | 800 μl / tube | 1 tube | 1600 μl / tube | 1 tube | Ndabere, nyocha, ihe nchekwa mmeghachi omume, dNTP |
| 4 | Ngwakọta enzyme SARS-CoV-2 | 80 μl / tube | 1 tube | 160 μl / tube | 1 tube | Mmalite Taq Enzyme dị ọkụ, M-MLV enzyme |
| 5 | SARS-CoV-2 ezigbo njikwa | 100 μl / tube | 1 tube | 100 μl / tube | 1 tube | Plasmid recombinant nwere iberibe ebumnuche, RNA |
| 6 | SARS-CoV-2 njikwa adịghị mma | 1200 μl / tube | 1 tube | 1200 μl / tube | 1 tube | Ihe nchekwa TE |
Atụmatụ & uru
Ngwa ngwa na mfe.
Zere mfu nucleic acid.
Oke nchọpụta dị ala na nghọta dị elu.
Achọrọ akụrụngwa dị ala yana iji mgbanwe dị ukwuu.
Nchọpụta nchọpụta nwere ike iru ọkwa kacha ala dị ka 500 mbipụta / ml).
| GCE/ml | Ct uru | |
| N gene | ORF1ab mkpụrụ ndụ ihe nketa | |
| 2.00E+08 | 16.43 | 15.16 |
| 2.00E+07 | 19.48 | 18.58 |
| 2.00E+06 | 22.71 | 21.86 |
| 2.00E+05 | 25.95 | 25.33 |
| 2.00E+04 | 29.30 | 28.49 |
| 2.00E+03 | 32.37 | 31.37 |
| 2.00E+02 | 35.32 | 34.47 |
| 2.00E+01 | 38.29 | 38.27 |
| 2.00E+00 | N/A | N/A |
| 2.00E-01 | N/A | N/A |
| NC | N/A | N/A |
Usoro mmụba nke ahịrị
Otu esi eji
1. Ngwa eji eme ihe
2. Usoro nchọpụta
Nhazi ihe nlele: Naopharyngeal ma ọ bụ oropharyngeal swab sample (naanị ọrụ nzọụkwụ 1) (Chere 10 mins)
PCR amplificaton (<= 55min)
Usoro ọrụ:
Nchekwa
mechie site na ìhè ma chekwaa na -20 ± 5 ℃;
Ndụ nchekwa:1 afọ
Ọ dị mma na-abịa 1st;ọrụ bụ nke mbụ;obere azụmahịa bụ imekọ ihe ọnụ” bụ anyị nzukọ nkà ihe ọmụma nke a na mgbe niile hụrụ na achụso site anyị ụlọ ọrụ maka N'ogbe OEM China Laboratory Nucleic Acid Extractor Extraction Reagent Kit maka PCR nchọpụta , Ezi obi olileanya na anyị na-eto eto ọnụ na ndị ahịa anyị n'ụwa nile.
OEM n'ogbeChina Nucleic Acid Extractor, Nucleic Acid mmịpụta, Akụrụngwa siri ike bụ mkpa inwe nzukọ ọ bụla.A na-akwado anyị site na akụrụngwa akụrụngwa siri ike nke na-enyere anyị aka imepụta, chekwaa, lelee mma na izipu ihe ngwọta anyị n'ụwa niile.Iji nọgide na-arụ ọrụ nke ọma, ugbu a, anyị ekewaala akụrụngwa anyị n'ọtụtụ ngalaba.Ngalaba ndị a niile na-arụ ọrụ na ngwa ọhụrụ, igwe na akụrụngwa emezigharịrị.N'ihi nke a, anyị nwere ike ịrụpụta nnukwu mmepụta na-emebighị ịdịmma ya.
