Ngwa Ngwa Mwepu DNA Bacterial Bacterial Genomic DNA Extraction Ngwa Ngwa dị ọcha
Nkọwa ngwa:
Mee ngwa ngwa DNA genomic dị elu site na nje bacteria na oge uto logarithmic.
◮Enweghị mmetọ RNase:Ogidi DNA-naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepụ RNA na DNA genomic na-agbakwunyeghị RNase n'oge nnwale ahụ, na-egbochi ụlọ nyocha ahụ site na RNase exogenous metọọ.
◮Ọsọ ọsọ:Foregene Protease nwere ọrụ dị elu karịa proteases yiri ya, ma na-agbari ihe nlele anụ ahụ ngwa ngwa;arụ ọrụ ahụ dị mfe, yana ọrụ mmịpụta DNA nke genomic nwere ike mechaa n'ime nkeji 20-80.
◮Dị mma:A na-eme centrifugation na okpomọkụ nke ụlọ, ọ dịghịkwa mkpa maka 4 Celsius C obere okpomọkụ centrifugation ma ọ bụ ethanol precipitation nke DNA.
◮Nchekwa:Ọ dịghị organic reagent mmịpụta a chọrọ.
◮Ịdị mma Dị elu:DNA genomic nke ewepụtara nwere nnukwu iberibe, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe nyocha dị iche iche chọrọ.
◮Sistemụ Micro-elution:Ọ nwere ike ịbawanye ntinye nke DNA genomic, nke dị mma maka nchọpụta ala ma ọ bụ nnwale.
Nkọwa
Ngwa a na-enye ngwa ngwa na ụzọ dị mfe iji wepụ DNA genomic site na omenala nke nje bacteria (Gram-negative na Gram-positive) site na isi mmalite dị iche iche;ọ nwere ike hazie ihe na-erughị 3ml nke omenala nje na oge uto logarithmic (bacteria 1 × 109).Ngwa ahụ dabara na Foregene Protease dị elu nke na-enye ohere 15-50μg nke DNA genomic dị elu ka ewepụtara n'ime elekere 1.Na mgbakwunye, ngwa ahụ nwekwara ike wepụ ihe mkpụrụ ndụ ihe nketa na-abụghị genome, dị ka plasmids, Cosmid, BAC, wdg.
Ihe DNA-naanị silica gel matrix ihe eji eme ihe na kọlụm spin bụ ihe ọhụrụ pụrụ iche nke ụlọ ọrụ ahụ, nke nwere ike ịgbasa DNA nke ọma na nke ọma.Ikike mgbasa ozi kachasị maka DNA bụ 80μg.Sistemụ nchekwa pụrụ iche na elution nwere ike ịbawanye mwepụ nke RNA, protein adịghị ọcha, ion na ogige organic ndị ọzọ na sel.Iberibe DNA genomic amịpụtara buru ibu, ịdị ọcha dị elu, kwụsiri ike na ịdị mma a pụrụ ịdabere na ya, na nha nke iberibe DNA kwụsiri ike na ihe dịka 23kb.
Nkọwapụta
Nkwadebe 50, nkwadebe 100, nkwadebe 250
Ngwa ngwa
| Ihe nchekwa ML1 |
| Ihe nchekwa ML2 |
| Ihe nchekwa PW |
| Ihe nchekwa WB |
| Ihe nchekwa EB |
| Protease nke mbụ |
| Lysozyme |
| Buffer TE |
| DNA-naanị kọlụm |
| Ntuziaka |
Atụmatụ & uru
-Ọnweghị mmetọ RNase: Ọ dịghị mkpa ịgbakwunye RNase iji wepụ RNA na DNA genomic, na-ezere ụlọ nyocha ahụ site na RNase exogenous.
-Ọsọ ọsọ ọsọ: Foregene Protease nwere ọrụ dị elu karịa proteases yiri ya ma nwee ike igbari ihe nlele nje gbajiri agbaji n'ime obere oge.
-dị mma: A na-eme centrifugation na ụlọ okpomọkụ, ọ dịghịkwa mkpa maka 4 Celsius C dị ala centrifugation ma ọ bụ ethanol precipitation nke DNA.
-Safety: ọ dịghị organic reagent mmịpụta a chọrọ.
-Ego dị elu: Iberibe DNA genomic amịpụtara buru ibu, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe achọrọ nnwale dị iche iche.
Ngwa ngwa
Ngwa a dabara adaba maka sachapụ DNA genomic nke ihe atụ ndị a: nje bacteria na-adịghị mma na nje gram-positive na oge uto logarithmic.
Usoro ọrụ
Nchekwa na Shelf ndụ
-Enwere ike ịchekwa ngwa a maka ọnwa 12 n'okpuru ọnọdụ akọrọ na ụlọ okpomọkụ (15-25 ° C);Ọ bụrụ na achọrọ ịchekwa ya ogologo oge, enwere ike ịchekwa ya na 2-8 Celsius.
Rịba ama: Ọ bụrụ na echekwara ya na obere okpomọkụ, ngwọta na-adịkarị mfe mmiri ozuzo.Tupu iji ya, jide n'aka na ị ga-etinye ngwọta na kit na ụlọ okpomọkụ ruo oge ụfọdụ.Ọ bụrụ na ọ dị mkpa, kpoo ya na mmiri ịsa mmiri 37 Celsius C maka nkeji 10 iji gbazee mkpọda ahụ, ma gwakọta ya tupu ejiri ya.
-Foregene Protease ngwọta nwere usoro pụrụ iche, nke na-arụ ọrụ mgbe echekwara na ụlọ okpomọkụ ruo ogologo oge (ọnwa 3);mgbe a na-echekwa ya na 4 Celsius, ọrụ ya na nkwụsi ike ga-aka mma, ya mere a na-atụ aro ka ịchekwa ya na 4 Celsius C, cheta ka ọ ghara idobe ya na -20 Celsius.
-Lysozyme bụ ntụ ntụ akọrọ, nke na-arụ ọrụ mgbe echekwara na 2-8 ° C ogologo oge (ọnwa 3).Maka nchekwa ogologo oge, biko chekwaa ya na -20 Celsius.
