Ngwa iche DNA na-enweghị cell
Nkọwa ngwa:
Nkọwa
DNA na-enweghị cell (cfDNA) bụ iberibe cfDNA ewepụtara site na mkpụrụ ndụ banye n'ọbara nke nwere ike ibu ozi ndu site na.etuto ahụ, pathogens na nwa ebu n'afọ.Nyocha nke cfDNA na-enye akara akara maka nchoputa, nchọpụta na nleba anya na ọganihu ọrịasion.Ugbu a, a na-eji teknụzụ a eme ihe n'ọtụtụ ebe n'ọhịa nchọpụta mmụgharị ụbụrụ, ntụzịaka ọgwụ ezubere iche na prognosis,nchọpụta ọrịa na-adịghị ahụkebe na nyocha tupu ọmụmụ nwa.
Emebere Kitdị Mwapụ DNA na-enweghị mkpụrụ ndụ Foregene maka ikewapụ DNA na-enweghị cell (cfDNA) na-ekesa na plasma mmadụ, ọbara ọbara, mmiri ahụ, ma ọ bụ ihe nlele mmamịrị.Ngwa ahụ nwere ike kewapụ cfDNA ngwa ngwa na nke dabara adaba na usoro atọ: lysis/jide, ịsacha na elution.Ọ na-eji beads superparamagnetic kpuchie silica emebere nke ọma nwere sistemu lysis/njikọ pụrụ iche iji nwetaghachi acid nucleic na-ekesa site na njikọ hydrogen na ike elektrọn, na-enweghị protein na-agbasa na adịghị ọcha ndị ọzọ.Ọ dabara maka ụlọ ọrụ mmịpụta akpaghị aka dị elu maka nkwadebe maka nyocha ọ bụla nke ala site na iji PCR ma ọ bụ NGS.
Ọdịnaya ngwa na Nchekwa
Tebụl 1 Ngwa Mwepu DNA na-enweghị mkpụrụ ndụ Foregene
| Cihe na-aga | Rdị ọkụ | Ọnụ ego(25 T) | Ọnụ ego(50 T) | Ọnụ ego(100 T) | Storage |
| Igbe 1 | Ihe nchekwa AL | 42 mlx1 karama | 89 mlx1 karama | 189 mLx1 karama | 1 0-30 Celsius C |
| Ihe nchekwa AW1 | 38 mlx1 karama | 83 mlx1 karama | 165 mlx1 karama | 1 0-30 Celsius C | |
| Ihe nchekwa AW2 | 15 mlx1 karama | 33 mlx1 karama | 66 mlx1 karama | 1 0-30 Celsius C | |
| Elution | 3 mlx1 karama | 6 mlx1 karama | 12 mlx1 karama | 1 0-30 Celsius C | |
| Igbe 2 | Igwe Igwe Igwe Ọdụdọ A | 3.2 mLx1 karama | 6.5 mLx1 karama | 13 mlx1 karama | 2-8 Celsius C |
| Proteinase K | 5 mlx1 karama | 10.5 mLx1 karama | 21 mlx1 karama | 2-8 Celsius C |
ndetu:
[1] Agwakọtala ihe ndị sitere na ngwa ngwa dị iche iche.
[2] Buffer AL nwere ike ịmalite ọnyà, nke anaghị emetụta ọrụ ya.Ọ bụrụ na a na-ahụ oke mmiri ozuzo, biko tinye karama reagent n'ime mmiri ịsa mmiri 56 Celsius C maka 1 0 - 20 min ruo mgbe mmiri na-agbaze.Mgbe ahụ gwakọta nke ọma tupu ejiri ya.
[3] Akwụsịla beads magnetik A.
Usoro
Atụmatụ & uru
-Mwepụta nke ọma Mkpụrụ mmịpụta DNA dị elu, ịdị ọcha dị mma, iwepụ ihe adịghị mma na ndị na-egbochi PCR dị ala;
Mgbanwe Sample IncorporationMfepụta cfDNA dị mfe site na nlele 0.5mL ruo 4mL nwere oke elution dị ala dị ka 20uL
Ngwa dị iche icheAkwadoro site na nlele ụlọọgwụ, emebere maka etuto, ọrịa na nchoputa nke ime nwa nwere biopsy mmiri mmiri
Nkwado sara mbara nke Platform akpaaka Dakọtara na akwụkwọ ntuziaka ans akpaghị aka usoro mmịpụta, nkwado Tecan, Hamilton, PE na ndị ọzọ nucleic acid mmịpụta workstations.
