Ọdịmma China dị mma nke ukwuu yana kwụsie ike Lyophilized Dspathtm 4X Otu nzọụkwụ Multiplex Master Mix
Nkọwa ngwa:
◮RNase inhibitor nke òké ewepụtara ọhụrụ gosipụtara na nje bacteria E. coli mebere site na iji teknụzụ recombination gene.Onye na-egbochi ihe na-egbochi ọrụ RNase site na ijikọghị asọmpi na RNase 1: 1, si otú a na-echebe.RNAiguzosi ike n'ezi ihe , ma nwee ike igbochi nke ọmankeRNase A, B, C ọrụ , ma ọ bụghị RNase T1, T2, H, wdg; Ọ bụ reversible nke njide nke RNase Inhibitor na RNase , na mgbagwoju nwere ike kewapụ site urea na sulfhydryl reagents, nke mere na RNase renatures na inhibitor bụ irreversibly inactivated.
Anyị nwere ike mgbe niile na-egbo anyị na-akwanyere ùgwù ahịa na anyị ezi elu àgwà, ezi price mkpado na ezi nkwado ruru ka anyị nwere ndị ọzọ ọkachamara na extra-arụsi ọrụ ike na-eme ya na-eri-irè ụzọ maka Excellent quality China Highly Efficient and Stable Lyophilized Dspathtm 4X One-Step Multiplex Master Mix, Jide n'aka na ị na-ahụ nnọọ nnọọ free ịkọrọ anyị maka nzukọ.anyị kwenyere na anyị ga-eso ndị ahịa anyị niile kerịta ahụmịhe azụmaahịa dị mma.
Anyị nwere ike na-egbo ndị ahịa anyị a na-akwanyere ùgwù mgbe niile na ezigbo mma anyị dị elu, mkpado ọnụahịa dị mma na nkwado dị mma n'ihi na anyị abụwo ọkachamara ọzọ na mgbakwunye na-arụsi ọrụ ike ma na-eme ya n'ụzọ dị ọnụ ala makaChina Taq DNA Polymerase, Qpcr, Ụlọ ọrụ anyị na-aga n'ihu na-ejere ndị ahịa ozi dị elu, ọnụahịa asọmpi na nnyefe oge.Anyị ji ezi obi na-anabata ndị enyi si n'akụkụ ụwa niile ka ha na anyị na-arụkọ ọrụ na ịbawanye azụmahịa anyị.Ọ bụrụ na ị nwere mmasị na ngwaahịa anyị, ị kwesịrị ị na-enwere onwe gị ịkpọtụrụ anyị.Ọ ga-amasị anyị ịnye gị ozi ndị ọzọ.
Nkọwapụta
5ml, 10ml, 40ml
PCR Hero (Ya na Dye) ngwa mmịpụta gụnyere ọgbọ ọhụrụ pụrụ iche nke Foregene Taq-Foregene Taq Hero, dNTP kachasị mma, MgCl2, ihe nchekwa mmeghachi omume, ihe nkwalite mmeghachi omume PCR, njikarịcha na ihe nkwụsi ike.Usoro 2 × PCR HeroTM Mix (ede) nwere nnabata dị elu nye ndị na-egbochi PCR karịa usoro PCR Mix nkịtị, ma nwee ike ịnagide PCR nkwalite dị iche iche dị mgbagwoju anya.Usoro mmeghachi omume pụrụ iche na Taq Hero dị elu na-eme ka mmeghachi omume PCR nwee arụmọrụ nkwalite dị elu, nkọwapụta na uche.
Ngwa ngwa
| 0.1U Taq Hero/μl |
| 400μM dNTPs |
| 20mM Tris-HCl (pH8.8) |
| 100mM KCl |
| 5mM mgCl2 |
| Ndị ọzọ stabilizers na enhancers |
| Ihe nchekwa DNA na-ebu |
Atụmatụ & uru
■ Ikwesị ntụkwasị obi dị elu: 6 ugboro nke enzyme Taq nkịtị;
■ Ọsọ nkwuwa okwu ngwa ngwa: ọsọ nkwalite bụ 5-10sec/kb, nke bụ 6-12 ugboro nke enzyme Taq nkịtị;
∎ Mgbanwe nke ndebiri: Ọ nwere ike ịkwalite ndebiri DNA dị mgbagwoju anya dị elu nke ọmaGC ọdịnaya;
■ Ịrụ ọrụ nkwalite dị elu: okirikiri mmụba dị ala karịa enzyme Taq nkịtị;
■ Nkwekọrịta gburugburu ebe obibi siri ike: etinye ya na 37 ° C maka otu izu, na-ejigide ihe karịrị 90% ọrụ;
■ Ọ nwere 5′ → 3′ DNA polymerase ọrụ na 5′→3′ exonuclease ọrụ, na-enweghị 3′ →5′ exonuclease ọrụ.
■ A na-ejikọta ngwa ahụ na Electrophoresis Loading Buffer, mgbe PCR gwụchara, enwere ike ibunye ihe nlele ahụ ozugbo maka electrophoresis;ọrụ ahụ dị mfe ma dị mfe.
Ngwa ngwa
Mmụba PCR nke iberibe DNA, akara DNA, usoro DNA, PCR tinyere A ọdụ
Nchekwa na Shelf ndụ
Enwere ike ịchekwa ya ogologo oge na -20 Celsius;maka ugboro ugboro, ọ nwere ike ịchekwa na 4 Celsius C maka obere oge. Anyị nwere ike mgbe niile na-egbo ndị ahịa anyị na-akwanyere ùgwù na anyị dị mma elu àgwà, ezi price mkpado na ezi nkwado ruru ka anyị nwere ndị ọzọ ọkachamara na extra hard-arụ ọrụ na-eme ya na-eri-irè ụzọ maka Excellent quality China Highly Efficient and Stable Lyophilized Dspathtm 4X One-Step sure you sense Multiplex for free Mixsolute Organisation.anyị kwenyere na anyị ga-eso ndị ahịa anyị niile kerịta ahụmịhe azụmaahịa dị mma.
Ogo mara oke mmaChina Taq DNA Polymerase, Qpcr, Ụlọ ọrụ anyị na-aga n'ihu na-ejere ndị ahịa ozi dị elu, ọnụahịa asọmpi na nnyefe oge.Anyị ji ezi obi na-anabata ndị enyi si n'akụkụ ụwa niile ka ha na anyị na-arụkọ ọrụ na ịbawanye azụmahịa anyị.Ọ bụrụ na ị nwere mmasị na ngwaahịa anyị, ị kwesịrị ị na-enwere onwe gị ịkpọtụrụ anyị.Ọ ga-amasị anyị ịnye gị ozi ndị ọzọ.
