With this motto in mind, we've turn into one of quite possibly the most technologically innovative, cost-official, and price-competitive manufacturers for factory Outlets for China High Efficiency PCR, Taq Plus DNA Polymerase , Na ụlọ ọrụ anyị na top quality to start with as our motto, we manufacture products that are entirely made in Japan, from material procurement to processing.Nke a na-enyere ha aka iji obi iru ala mee ihe.
N'iburu ụkpụrụ a n'uche, anyị aghọọla otu n'ime ndị nrụpụta teknụzụ kachasị ọhụrụ, ndị na-akwụ ụgwọ dị oke ọnụ na ndị na-asọmpi maka ọnụahịa.China PCR Amplification, Qpcr, Ọkachamara, Devoting bụ mgbe ndabere anyị ozi.Anyị na-adị mgbe niile na-eje ozi ahịa, na-eke uru management ebumnobi na-agbasosi ezi obi, nraranye, na-adịgide adịgide management echiche.

Nkọwapụta

50 × 20μl rxns, 200 × 20μl rxns, 1000 × 20μl rxns, 2000 × 20μl rxns.

2X Real PCR EasyTM Mix-Taqman nke Real Time PCR EasyTM-Taqman kit na-enye bụ usoro premix ọhụrụ nke na-eji nyocha fluorescent akọwapụtara maka mmeghachi omume nkwalite PCR Real Time, nke nwere ike melite nkọwapụta ngwaahịa na mmetụta mmeghachi omume.A na-enye ROX ka ọ bụrụ ihe eji agba agba n'ime.

2X Real PCR EasyTM Mix-Taqman nwere mmalite mmalite Taq DNA Polymerase pụrụ iche nke Foregene.N'iji ya na enzymes Taq nkịtị, ọ nwere uru dị na nrụpụta nkwalite dị elu, ikike nkwalite siri ike yana ọnụ ọgụgụ na-adaghị adaba.Ọ nwere ike belata mmụba na-abụghị nke akọwapụtara ma melite izi ezi nke PCR.

Ngwa ngwa

Atụmatụ & uru

∎ Mfe—2X PCR Ngwakọta iji belata njehie nnwale na oge ọrụ

∎ Specific—nkwadebe nke kachasị na enzyme Taq mmalite na-ekpo ọkụ nwere ike igbochi nkwalite na-abụghị nke akọwapụtara na nhazi ọkwa mbụ.

∎ Mmetụta dị elu-nwere ike ịchọpụta obere ndebiri nke ndebiri

∎ Ọdịiche dị mma—dakọtara na ọtụtụ ngwa PCR ọnụ ọgụgụ dị adị

Ngwa ngwa

qPCR nyocha

Usoro ọrụ

Eserese

Nchekwa na Shelf ndụ

Ekwesịrị ịchekwa ngwa a n'ebe dịpụrụ adịpụ na ọkụ ma debe ya na -20 ℃.Ọ bụrụ na-eji ugboro ugboro, ọ nwekwara ike na-echekwara na 4 ℃ maka a obere oge nke oge (10 ụbọchị) .Na nke a motto n'uche, anyị na-atụgharị n'ime otu nke nnọọ ikekwe kasị technologically ọhụrụ, na-eri-oru oma, na price-asọmpi emepụta maka factory Outlets maka China High Efficiency PCR, Taq Plus DNA Polymerase#P10032, na-amalite na anyị na-arụ ọrụ na-arụ ọrụ nke ọma, na-amalite na anyị na-emepụta ihe na-emepụta ihe niile. Emebere ya na Japan, site na ịzụrụ ihe ruo na nhazi.Nke a na-enyere ha aka iji obi iru ala mee ihe.
ụlọ ọrụ mmepụta ihe makaChina PCR Amplification, Qpcr, Ọkachamara, Devoting bụ mgbe ndabere anyị ozi.Anyị na-adị mgbe niile na-eje ozi ahịa, na-eke uru management ebumnobi na-agbasosi ezi obi, nraranye, na-adịgide adịgide management echiche.

Enweghị mgbama nkwalite

1.Taq DNA Polymerase na ngwa ahụ na-efunahụ ọrụ ya n'ihi nchekwa na-ezighị ezi ma ọ bụ njedebe nke ngwa ahụ.
Nkwanye: Kwado ọnọdụ nchekwa nke ngwa ahụ;tinyeghachi Taq DNA Polymerase kwesịrị ekwesị na sistemu PCR ma ọ bụ zụta ngwa PCR Real Time ọhụrụ maka nnwale ndị metụtara ya.

2.E nwere ọtụtụ ihe mgbochi nke Taq DNA Polymerase na DNA template.
Aro: Megharịa ndebiri ma ọ bụ belata ọnụọgụ ndebiri ejiri.

3.The Mg2 + ịta adịghị adabara.
Nkwanye: Mg2 + ntinye nke the2 × Real PCR Mix anyị na-enye bụ 3.5mM.Agbanyeghị, maka ụfọdụ primers na ndebiri pụrụ iche, ntinye Mg2+ nwere ike ịdị elu.Ya mere, ị nwere ike tinye MgCl2 ozugbo iji bulie mkpokọta Mg2+.A na-atụ aro ka ịbawanye Mg2+ 0.5mM oge ọ bụla maka njikarịcha.

4.The PCR amplification ọnọdụ adịghị adabara, na primer usoro ma ọ bụ itinye uche na-ekwesịghị ekwesị.
Aro: gosi izi ezi nke usoro nke primer ma emebiela primer;Ọ bụrụ na mgbama nkwuwapụta adịghị mma, gbalịa iwetulata okpomọkụ na-ekpo ọkụ ma mezie ntinye nke primer nke ọma.

5. Ọnụ ọgụgụ nke template dị ntakịrị ma ọ bụ nke ukwuu.
Nkwanye: Mee template linearization gradient dilution, wee họrọ mkpokọta template nwere mmetụta PCR kacha mma maka nnwale PCR oge.

NTC nwere oke fluorescence uru

1.Reagent mmetọ mere n'oge ọrụ.
Nkwanye: Dochie na ọhụrụ reagents maka ezigbo oge PCR nnwale.

2.Contamination mere n'oge nkwadebe nke usoro mmeghachi omume PCR.
Nkwanye: Were usoro nchebe dị mkpa mgbe ị na-arụ ọrụ, dị ka: iyi uwe latex, iji ntu pipette nwere nzacha, wdg.

3.The primers na-eweda n'ala, na mmebi nke primers ga-eme ka ndị na-abụghị kpọmkwem amplification.
Aro: Jiri SDS-PAGE electrophoresis chọpụta ma ọ̀tụ̀tụ̀tụ̀ ihe ndị e ji emepụta ihe na-emebi emebi, ma jiri primers ọhụrụ dochie ha maka nnwale PCR Real Time.

Primer dimer ma ọ bụ nkwalite na-abụghị nke akọwapụtara

1.The Mg2 + ịta adịghị adabara.
Nkwanye: Mg2 + ntinye uche nke 2 × Real PCR EasyTM Mix anyị na-enye bụ 3.5 mM.Agbanyeghị, maka ụfọdụ primers na ndebiri pụrụ iche, ntinye Mg2+ nwere ike ịdị elu.Ya mere, ị nwere ike tinye MgCl2 ozugbo iji bulie mkpokọta Mg2+.A na-atụ aro ka ịbawanye Mg2+ 0.5mM oge ọ bụla maka njikarịcha.

2.The PCR annealing okpomọkụ dị oke ala.
Aro: Mee ka PCR annealing okpomọkụ site 1℃ ma ọ bụ 2℃ oge ọ bụla.

3.The PCR ngwaahịa dị ogologo.
Nkwanye: Ogologo ngwaahịa PCR Real Time kwesịrị ịdị n'etiti 100-150bp, ọ bụghị karịa 500bp.

4.The primers na-eweda n'ala, na nbibi nke primers ga-eduga na ọdịdị nke kpọmkwem mmụba.
Aro: Jiri SDS-PAGE electrophoresis chọpụta ma ọ̀tụ̀tụ̀tụ̀ ihe ndị e ji emepụta ihe na-emebi emebi, ma jiri primers ọhụrụ dochie ha maka nnwale PCR Real Time.

5.The PCR usoro na-ekwesịghị ekwesị, ma ọ bụ usoro dị oke obere.
Aro: Sistemụ mmeghachi omume PCR pere mpe ga-eme ka nchọta nke ọma belata.Ọ kacha mma iji usoro mmeghachi omume nke akụrụngwa PCR ọnụọgụ tụrụ aro iji megharịa nnwale PCR Real Time.

Nkwughachi nke ụkpụrụ ọnụọgụgụ adịghị mma

1. The ngwá bụ malfunctioning.
Aro: Enwere ike inwe mperi n'etiti oghere PCR ọ bụla nke ngwaọrụ ahụ, na-ebute mmeghari na-adịghị mma n'oge njikwa okpomọkụ ma ọ bụ nchọpụta.Biko lelee dị ka ntuziaka nke ngwa kwekọrọ.

2.The sample ịdị ọcha adịghị mma.
Nkwanye: Ihe nlele na-adịghị ọcha ga-eduga na mmeghari nke nnwale ahụ na-adịghị mma, nke gụnyere ịdị ọcha nke template na primers.Ọ kacha mma imegharị ndebiri ahụ, yana SDS-PAGE na-asachapụ ihe ndị ahụ.

3.The PCR usoro nkwadebe na oge nchekwa dị ogologo.
Aro: Jiri ezigbo oge PCR usoro maka PCR nnwale ozugbo nkwadebe, na ahapụla ya n'akụkụ ruo ogologo oge.

4.The PCR amplification ọnọdụ adịghị adabara, na primer usoro ma ọ bụ itinye uche na-ekwesịghị ekwesị.
Aro: gosi izi ezi nke usoro nke primer ma emebiela primer;Ọ bụrụ na mgbama nkwuwapụta adịghị mma, gbalịa iwetulata okpomọkụ na-ekpo ọkụ ma mezie ntinye nke primer nke ọma.

5.The PCR usoro na-ekwesịghị ekwesị, ma ọ bụ usoro dị oke obere.
Aro: Sistemụ mmeghachi omume PCR pere mpe ga-eme ka nchọta nke ọma belata.Ọ kacha mma iji usoro mmeghachi omume nke akụrụngwa PCR ọnụọgụ tụrụ aro iji megharịa nnwale PCR Real Time.