Ọrụ anyị bụ inye ndị ọrụ njedebe anyị na ndị ahịa ahịa ahịa dijitalụ kacha mma na asọmpi mbugharị maka ụlọ ọrụ na-eweta Nucleic Acid Extraction Machine Automated Medical Equipment Nucleic Acid Extraction System Rna/DNA Extraction Instrument, Ndị ahịa anyị na-ekesa n'ime North America, Africa na Eastern Europe.anyị na-enwe ike ịnye ngwa ahịa dị oke mma na mkpado ọnụahịa dị egwu na-enweghị atụ.
Ọrụ anyị bụ inye ndị ọrụ njedebe anyị na ndị ahịa ahịa ngwa ahịa dijitalụ kacha mma dị elu yana asọmpi maka yaIgwe mwepu nke China na Nnwale Acid Nucleic, Ugbu a, anyị ebupụla ngwongwo anyị n'ụwa niile, karịsịa USA na mba Europe.Ọzọkwa, a na-emepụta ihe niile anyị na-eji ngwá ọrụ dị elu na usoro QC siri ike iji hụ na ọ dị elu. Ọ bụrụ na ị nwere mmasị na ngwa ahịa anyị ọ bụla, cheta na ị gaghị egbu oge ịkpọtụrụ anyị.Anyị ga-agbalị ike anyị niile igbo mkpa gị.
Akwụkwọ ntuziaka:

Ọrụ anyị bụ inye ndị ọrụ njedebe anyị na ndị ahịa ahịa ahịa dijitalụ kacha mma na asọmpi mbugharị maka ụlọ ọrụ na-eweta Nucleic Acid Extraction Machine Automated Medical Equipment Nucleic Acid Extraction System Rna/DNA Extraction Instrument, Ndị ahịa anyị na-ekesa n'ime North America, Africa na Eastern Europe.anyị na-enwe ike ịnye ngwa ahịa dị oke mma na mkpado ọnụahịa dị egwu na-enweghị atụ.
Ụlọ ọrụ wetaraIgwe mwepu nke China na Nnwale Acid Nucleic, Ugbu a, anyị ebupụla ngwongwo anyị n'ụwa niile, karịsịa USA na mba Europe.Ọzọkwa, a na-emepụta ihe niile anyị na-eji ngwá ọrụ dị elu na usoro QC siri ike iji hụ na ọ dị elu. Ọ bụrụ na ị nwere mmasị na ngwa ahịa anyị ọ bụla, cheta na ị gaghị egbu oge ịkpọtụrụ anyị.Anyị ga-agbalị ike anyị niile igbo mkpa gị.

Ntuziaka nyocha nsogbu

Ihe na-esonụ bụ nyocha nke nsogbu ndị nwere ike izute na mmịpụta nke DNA/RNA viral, na-atụ anya na ọ ga-enyere gị aka na nnwale gị.Na mgbakwunye, maka nsogbu nnwale ma ọ bụ teknụzụ ndị ọzọ karịa ntuziaka ọrụ na nyocha nsogbu, anyị ewepụtala nkwado teknụzụ iji nyere gị aka.Ọ bụrụ na ị nwere mkpa ọ bụla, biko kpọtụrụ anyị: 028-83360257 ma ọ bụ E-mail:

Tech@foregene.com.

Enweghị mmịpụta nucleic acid ma ọ bụ mkpụrụ nucleic acid dị ala

A na-enwekarị ọtụtụ ihe na-emetụta arụmọrụ mgbake, dị ka: sample ọdịnaya nucleic acid, usoro ọrụ, elution volume, wdg.

Nyocha nke ihe na-akpatakarị:

1. A na-eme centrifugation ice bat ma ọ bụ obere okpomọkụ (4 ° C) n'oge usoro ahụ.

Aro: Na-arụ ọrụ na ụlọ okpomọkụ (15-25ºC) n'ime usoro dum, emela ice bat na obere okpomọkụ centrifugation.

2. A na-echekwa ihe nlele ahụ na-ekwesịghị ekwesị ma ọ bụ na-echekwa ihe nlele ahụ ogologo oge.

Nkwanye: Chekwaa ihe nlele na -80 Celsius ma zere ịfriza ugboro ugboro na thawing;gbalịa iji ihe nlele achịkọtara ọhụrụ maka mmịpụta acid nucleic.

3. ezughị ezu sample lysis.

Nkwanye: Biko hụ na ihe nlele na ngwọta na-arụ ọrụ lysis na-agwakọta nke ọma ma tinye ya na ụlọ okpomọkụ (15-25 ° C) maka nkeji 10.

4. mgbakwunye na-ezighi ezi nke eluent.

Aro: Gbaa mbọ hụ na agbakwunyere RNase-Free ddH2O dabara n'etiti akpụkpọ ahụ kọlụm dị ọcha, ma etinyekwala ya na mgbanaka kọlụm dị ọcha.

5. Agbakwunyeghị olu ziri ezi nke ethanol zuru oke na Buffer RW2.

Aro: Biko soro ntuziaka a, tinye nha ziri ezi nke ethanol zuru oke na Buffer RW2 wee gwakọta nke ọma tupu i jiri ngwa ahụ.

6. Mpịakọta ihe atụ na-ekwesịghị ekwesị.

Aro: A na-ahazi 200µl nke nlele maka 500µl nke ihe nchekwa DRL ọ bụla.Nhazi ihe nlere nke ukwuu ga-eme ka mkpụrụ nke mmịpụta nucleic acid dị ala.

7. Mpịakọta elution na-ekwesịghị ekwesị ma ọ bụ elution ezughị ezu.

Nkwanye: Oke eluent nke kọlụm nchacha bụ 30-50μl;Ọ bụrụ na mmetụta elution adịghị afọ ojuju, a na-atụ aro ịgbatị oge n'ime ụlọ okpomọkụ mgbe agbakwunyere ddH2O na-enweghị ọkụ RNase, dị ka 5-10min.

8. Ethanol na-anọgide na kọlụm mgbe ịsachara na Buffer RW2.

Aro: Ọ bụrụ na ethanol na-anọgide mgbe centrifugation na Buffer RW2 maka nkeji 2, a ga-etinye kọlụm n'ime ụlọ okpomọkụ maka nkeji 5 mgbe centrifugation gachara iji wepụ ethanol fọdụrụnụ kpamkpam.

Nucleic acid a na-eme ka ọ dị ọcha na-emebi

Ogo nke nucleic acid a na-eme ka ọ dị ọcha metụtara ichekwa ihe nlele ahụ, mmetọ RNase, ọrụ na ihe ndị ọzọ.Nyocha nke ihe na-akpatakarị:

1. A naghị echekwa ihe nlele ndị anakọtara na oge.

Aro: Ọ bụrụ na ejighị ihe nlele ahụ n'oge nchịkọta, biko chekwaa ya na -80 Celsius na obere okpomọkụ ozugbo.Maka mmịpụta RNA, gbalịa iji ihe nlele anakọtara ọhụrụ.

2. Na-anakọta ihe nlele na ifriizi ma mebie ugboro ugboro.

Aro: Zere oyi na thawing (ọ bụghị ihe karịrị otu ugboro) n'oge nchịkọta na nchekwa nke samples, ma ọ bụghị ya, mkpụrụ nucleic acid ga-ebelata.

3. A na-ewebata RNase n'ime ụlọ ọrụ ma ọ bụ uwe mkpuchi, ihe mkpuchi, wdg.

Nkwanye: A na-eme nnwale mmịpụta RNA kacha mma n'ime ụlọ ọrụ RNA dị iche, yana tebụl ụlọ nyocha kwesịrị ihicha tupu nnwale ahụ.

Yiri gloves na masks ndị enwere ike iwepụ n'oge nnwale ahụ ka ịzenarị mmebi nke RNA kpatara site na iwebata RNase ruo oke.

4. Eji RNase metọọ reagent mgbe eji ya.

Nkwanye: Dochie na Viral DNA/RNA Isolation Kit ọhụrụ maka nnwale ndị metụtara ya.

5. A na-eji RNase metọọ tubes centrifuge na ndụmọdụ pipette eji eme ihe maka RNA.

Aro: Jide n'aka na tubes centrifuge, ndụmọdụ pipette, pipettes, wdg. ejiri maka mmịpụta RNA bụ ihe niile RNase enweghị.

Nucleic acid dị ọcha na-emetụta nnwale ndị dị n'okpuru ala

DNA na RNA sachara site na kọlụm nchacha, ọ bụrụ na ion nnu na protein dị elu, ọ ga-emetụta nnwale ndị dị n'okpuru ala, dịka: nkwalite PCR, ntụgharị ntụgharị, wdg.

1. DNA nke eluted na RNA nwere ion nnu fọdụrụnụ.

Aro: Gbaa mbọ hụ na agbakwunyere olu ziri ezi nke ethanol zuru oke na Buffer RW2, wee saa kọlụm nchacha ugboro abụọ na ọsọ centrifugation akọwapụtara na ntuziaka ọrụ;Mee centrifugation iji belata mmetọ ion nnu.

2. DNA na RNA na-elu elu nwere ethanol residues.

Aro: Mgbe akwadochara ịsa ahụ na Buffer RW2, mee centrifugation tube efu na ọsọ centrifugation na ntuziaka ọrụ;Ọ bụrụ na a ka nwere ihe fọdụrụ na ethanol, ị nwere ike centrifuge tube efu wee tinye ya na ụlọ okpomọkụ maka nkeji 5 iji wepụ ihe fọdụrụ ethanol ruo n'ókè kasị ukwuu.

Akwụkwọ ntuziaka:

Akwụkwọ ntuziaka ngwa mwepu nke DNA na RNA