All we do is usuallyconnected with our tenet ” Consumer initial, Rely on 1st, devoting around the food stuff packaging and environment safe for High Performance China Magpure Viral Nucleic Acid Isolation Kit with Preassembly 96 Well Plate, Our tenet is evident each of the time: to provide good quality solution at competitive rate to clients around the planet.Anyị na-anabata ndị nwere ike ịzụrụ anyị ka ha kpọọ anyị maka iwu OEM na ODM.
Ihe niile anyị na-eme na-ejikọkarị ya na tenet anyị ” mbụ ndị ahịa, dabere na 1st, na-etinye gburugburu nkwakọ ngwaahịa nri na nchekwa gburugburu ebe obibi makaChina Rna/DNA Mwepụta/Nsacha, Mmanya Rna iche, N'ime afọ 11, anyị na-ekere òkè na ihe ngosi 20 karịa, na-enweta otuto kachasị elu site n'aka ndị ahịa ọ bụla.Ụlọ ọrụ anyị na-achọ mgbe niile ịnye ndị ahịa ngwaahịa kacha mma na ọnụ ala dị ala.Anyị na-agbasi mbọ ike iji nweta ọnọdụ mmeri a ma jiri ezi obi nabata gị isonyere anyị.Soro anyi, gosi mma gi.Anyị ga-abụ nhọrọ mbụ gị mgbe niile.Tụkwasa anyị obi, ị gaghị ada mbà.

Nkọwa ngwa ngwa

Nkwadebe 50, 200 Preps

Ngwa a na-eji kọlụm spine na usoro nke ụlọ ọrụ anyị mepụtara, nke nwere ike wepụ ịdị ọcha dị elu na nke dị elu RNA site na anụ ahụ dị iche iche nke anụmanụ na-arụ ọrụ dị elu. Ọ na-enye DNA-Cleaning Column na-arụ ọrụ nke ọma, nke nwere ike ikewapụ na adsorb genomic DNA site na supernatant na anụ ahụ lysate, dị mfe na-azọpụta oge;Kọlụm naanị RNA nwere ike jikọta RNA nke ọma ma nwee ike hazie ya n'otu oge site na iji usoro pụrụ iche Ọtụtụ nlele.

Usoro dum bụ RNase-Free, nke mere na RNA amịpụtara adịghị emebi emebi;Buffer RW1, Buffer RW2 sistemu ịsacha ihe, nke mere na RNA enwetara enweghị protein, DNA, ion, na mmetọ ngwakọta organic.

Ngwa ngwa:

Atụmatụ & uru

■ Ọ dịghị mkpa ichegbu onwe gị maka mbibi RNA;Sistemụ niile bụ RNase-Free
∎ Wepu DNA nke ọma site na iji kọlụm na-ehicha DNA
■ Wepu DNA na-agbakwunyeghị DNA
■ A na-arụ ọrụ niile dị mfe na ọnụ ụlọ okpomọkụ
■ Enwere ike ịrụcha ọrụ ngwa ngwa n'ime nkeji iri atọ
■ Nchekwa-ọ dịghị ihe reagent organic achọrọ
■ Ịdị ọcha dị elu -OD260 / 280≈1.8-2.1

Ngwa ngwa

Ọ dabara maka mmịpụta na ịdị ọcha nke mkpokọta RNA site na anụ ahụ dị ọhụrụ ma ọ bụ oyi kpọnwụrụ dị iche iche ma ọ bụ sel emebere.

Ngwa ngwaahịa

∎ Ngwa mgbada ala: njikọ cDNA nke mbụ, RT-PCR, cloning molecular, Northern Blot, wdg.
■ Ihe nlele: anụ ahụ anụmanụ, mkpụrụ ndụ omenala
■ Usoro onunu ogwu: Tissues 10-20mg, Sel (2-5) × 10⁶
∎ Oke kachasị ike ijikọ DNA nke kọlụm nchacha: 80 μg
■ Olu olu: 50-200 μl

Usoro ọrụ

Eserese

Ngwakọta Mkpokọta RNA nke anụmanụ na-agwọ 20mg
Ihe nlele òké ọhụrụ, were 5% RNA 1% agar sachara ọcha

Glycogel electrophoresis
1: Spleen 2: Akụrụ
3: Imeju 4: Obi

Nchekwa na ndụ nchekwa

Enwere ike ịchekwa ngwa ahụ maka ọnwa 24 na okpomọkụ nke ụlọ (15-25 ℃) ma ọ bụ 2-8 ℃ maka ogologo oge.Buffer RL1 nwere ike ịchekwa na 4 ℃ maka 1 ọnwa mgbe agbakwunyere β-mercaptoethanol (nhọrọ) .Ihe niile anyị na-eme na-ejikarị anyị tenet ” Consumer initial, ịdabere na 1st, devoting around the food stuff packaging na nchekwa gburugburu ebe obibi maka High Performance China Magpure Viral Nucleic Acid Isolation Kit na Preassembly nke ọ bụla na-enye ihe ngwọta dị mma na Preassembly. ọnụ ọgụgụ asọmpi nye ndị ahịa n'ụwa niile.Anyị na-anabata ndị nwere ike ịzụrụ anyị ka ha kpọọ anyị maka iwu OEM na ODM.
Ịrụ ọrụ dị eluChina Rna/DNA Mwepụta/Nsacha, Mmanya Rna iche, N'ime afọ 11, anyị na-ekere òkè na ihe ngosi 20 karịa, na-enweta otuto kachasị elu site n'aka ndị ahịa ọ bụla.Ụlọ ọrụ anyị na-achọ mgbe niile ịnye ndị ahịa ngwaahịa kacha mma na ọnụ ala dị ala.Anyị na-agbasi mbọ ike iji nweta ọnọdụ mmeri a ma jiri ezi obi nabata gị isonyere anyị.Soro anyi, gosi mma gi.Anyị ga-abụ nhọrọ mbụ gị mgbe niile.Tụkwasa anyị obi, ị gaghị ada mbà.

A naghị ewepụta RNA ma ọ bụ RNA dị ala

A na-enwekarị ihe dị iche iche na-emetụta arụmọrụ mgbake, dịka: ọdịnaya RNA anụ ahụ, usoro ọrụ, elution volume, wdg.

1. Ice bath ma ọ bụ cryogenic (4 Celsius C) centrifugation mere n'oge ọrụ.

Nkwanye: Na-arụ ọrụ na ụlọ okpomọkụ (15-25 Celsius C) n'ime usoro dum, emela ice bat na centrifuge na obere okpomọkụ.

2. Nchekwa nlele na-ezighi ezi ma ọ bụ oge nchekwa oke.

Nkwanye: Chekwaa ihe nlele na -80 Celsius ma ọ bụ ifriizi na mmiri mmiri nitrogen ma zere iji friza eme ihe ugboro ugboro;gbalịa iji anụ ahụ ọhụrụ ma ọ bụ mkpụrụ ndụ emebere maka mmịpụta RNA.

3. ezughị ezu sample lysis.

Nkwanye: Mgbe ị na-eme ka anụ ahụ dị n'otu, hụ na anụ ahụ na-emekọ ihe nke ọma yana na mkpụrụ ndụ anụ ahụ kewara nke ọma iji kọwaa ntọhapụ nke RNA.

4. A naghị atụkwasị eluent nke ọma.

Nkwanye: Kwenye na ddH enweghị RNase₂A na-agbakwunye O n'ime ala n'etiti akpụkpọ ahụ kọlụm nke ịdị ọcha.

5. Agbakwunyeghị olu ziri ezi nke ethanol zuru oke na Buffer RL2 ma ọ bụ Buffer RW2.

Nkwanye: Soro ntuziaka a, tinye nha ziri ezi nke ethanol zuru oke na Buffer RL2 na Buffer RW2 wee gwakọta nke ọma tupu i jiri ngwa ahụ.

6. Usoro ihe nlele anụ ahụ adịghị mma.

Nkwanye: Jiri 10-20 mg nke anụ ahụ ma ọ bụ (1-5) × 10⁶mkpụrụ ndụ kwa 500 μl ihe nchekwa RL1, n'ihi na iji anụ ahụ gabigara ókè nwere ike ime ka mmịpụta RNA belata.

7. Olu elution na-ezighi ezi ma ọ bụ elution ezughị ezu.

Nkwanye: Oke elution nke kọlụm nchacha bụ 50-200 μl;Ọ bụrụ na mmetụta elution adịghị afọ ojuju, a na-atụ aro ịgbatị oge ntinye ụlọ okpomọkụ mgbe agbakwunyere ddH na-enweghị ọkụ RNase.₂O, ọmụmaatụ maka nkeji 5-10.

8.The kọlụm dị ọcha nwere ethanol fọdụrụnụ mgbe Buffer RW2 saa.

Nkwanye: Ọ bụrụ na e nwere ihe fọdụrụ na ethanol mgbe ịsachara Buffer RW2, centrifugation tube efu maka 1min, enwere ike ịbawanye oge ọrụ centrifugation tube efu ruo 2min, ma ọ bụ enwere ike idobe kọlụm dị ọcha na ụlọ okpomọkụ maka 5 min iji wepụ ethanol fọdụrụnụ nke ọma.

RNA dị ọcha na-eweda ya ala

Àgwà nke RNA a sachara ejikọta na ihe ndị dị ka ichekwa ihe nlele, mmetọ RNase, na nhazi, wdg.

1. A naghị edebe ihe nlele anụ ahụ n'oge.

Nkwanye: Ọ bụrụ na ejighị ihe nlele anụ ahụ ma ọ bụ sel mee ihe n'oge kwesịrị ekwesị mgbe nchịkọtachara, ozugbo cryopreserve na -80 Celsius C ma ọ bụ nitrogen mmiri mmiri.Iji wepụ RNA, jiri anụ ahụ ma ọ bụ ihe nlele cell ewepụtara ọhụrụ mgbe ọ bụla enwere ike.

2. Ugboro ugboro ifriizi-thawing nke anụ ahụ sample.

Nkwanye: Mgbe ị na-echekwa ihe nlele anụ ahụ, ọ kacha mma ịkpụ ya n'ime obere iberibe maka nchekwa, ma wepụ otu n'ime iberibe ahụ mgbe ị na-eji ha ka ịzenarị oyiri ugboro ugboro nke ihe nlele ahụ na mmebi nke RNA.

3. A na-ewebata RNase ma ọ bụ ghara iyi uwe mkpuchi, ihe mkpuchi, wdg n'oge ọrụ ahụ.

Nkwanye: A na-eme nnwale mmịpụta RNA kacha mma n'ime ụlọ njikwa RNA dị iche iche wee kpochapụ tebụl tupu nnwale ahụ.

Yiri gloves na masks enwere ike iwepụ n'oge nnwale ahụ iji belata mmebi RNA kpatara site na iwebata RNase.

4. A na-eji RNase metọọ reagents n'oge eji.

Nkwanye: Dochie jiri ngwa Mkpokọta anụ ọhịa RNA ọhụrụ maka nnwale ndị metụtara ya.

5. Ọkpụkpọ centrifuge, ndụmọdụ, wdg eji eme ihe na RNA na-emetọ ya na RNase.

Nkwanye: Kwenye na tubes centrifuge, ndụmọdụ, pipettes, wdg. ejiri na mmịpụta RNA bụ RNase niile.

RNA emepụtara dị ọcha na-emetụta nnwale ndị dị n'okpuru ala

RNA sachara site na kọlụm dị ọcha, ọ bụrụ na ion nnu, ọdịnaya protein buru ibu ga-emetụta nnwale nke ala, dị ka: ntụgharị ntụgharị, Northern Blot et al.

1. RNA nke elutioned nwere residues ion nnu.

Nkwanye: Kwenye na agbakwunyere oke ethanol ziri ezi na Buffer RW2 ma mee 2 kọlụm nchacha ọcha na ọsọ centrifugal nke egosiri maka ịrụ ọrụ;Ọ bụrụ na enwere ihe fọdụrụ ion nnu, hapụ kọlụm nke ịdị ọcha na Buffer RW2 maka 5 min na ụlọ okpomọkụ ma mee centrifugation iji bulie mwepụ nke mmetọ nnu.

2. Ethanol fọdụrụnụ na elutioned RNA.

Nkwanye: Kwenye na mgbe ịsachara RW2 na-enweghị ihe ọ bụla, rụọ ọrụ centrifugation tube efu na ọsọ centrifugation egosipụtara maka ịrụ ọrụ, mụbaa oge ọrụ centrifugation tube efu ruo 2 min ma ọ bụrụ na ọ ka nwere ihe fọdụrụ na ethanol, ma ọ bụ hapụ ya n'ime ụlọ okpomọkụ maka 5 min mgbe centrifugation efu gachara iji bulie mwepụ nke ethanolue.