We offer wonderful energy in high-quality and improve,merchandising,product sales and marketing and advertising and process for Manufacturer for Single Strip Mag-Rod Comb Nucleic Acid Test Detection disposable Consumables, Our Enterprise insists on innovation to promote the sustainable development of company, and make us become the domestic high-quality suppliers.
Anyị na-enye ike magburu onwe ya na elu-edu na mma, ịzụ ahịa, ngwaahịa ahịa na ahịa na mgbasa ozi na usoro makaChina Nucleic Acid na ọgwụ oriri, Na-eche ihu kpụ ọkụ n'ọnụ zuru ụwa ọnụ ahịa asọmpi, anyị na-na na ulo oru na ika ụlọ atụmatụ na emelitere mmụọ nke "mmadụ na-adabere na kwesịrị ntụkwasị obi ọrụ", na nzube iji nweta zuru ụwa ọnụ ude na-adigide mmepe.

Nkọwa Kit

2X Real PCR dị mfe^TMMix-Taqman nke ezigbo oge PCR dị mfe^TM-Taqman kit bụ usoro premix ọhụrụ nke na-eji nyocha fluorescent akọwapụtara maka mmeghachi omume nkwalite PCR Real Time, nke nwere ike melite nkọwapụta ngwaahịa na mmetụta mmeghachi omume.A na-enye ROX ka ọ bụrụ ihe eji agba agba n'ime.

2X Ezigbo PCR dị mfe^TMMix-Taqman nwere ihe mmalite Taq DNA Polymerase pụrụ iche nke Foregene.N'iji ya na enzymes Taq nkịtị, ọ nwere uru dị na nrụpụta nkwalite dị elu, ikike nkwalite siri ike yana ọnụ ọgụgụ na-adaghị adaba.Ọ nwere ike belata mmụba na-abụghị nke akọwapụtara ma melite izi ezi nke PCR.

Nkọwapụta

Atụmatụ & uru

∎ Mfe—2X PCR Ngwakọta iji belata njehie nnwale na oge ọrụ

∎ Specific—nkwadebe nke kachasị na enzyme Taq mmalite na-ekpo ọkụ nwere ike igbochi nkwalite na-abụghị nke akọwapụtara na nhazi ọkwa mbụ.

∎ Mmetụta dị elu-nwere ike ịchọpụta obere ndebiri nke ndebiri

∎ Ọdịiche dị mma—dakọtara na ọtụtụ ngwa PCR ọnụ ọgụgụ dị adị

Ngwa ngwa

qPCR nyocha

Usoro ọrụ

Eserese

Nchekwa na ndụ nchekwa

Ekwesịrị ịchekwa ngwa a n'ebe dịpụrụ adịpụ na ọkụ ma debe ya na -20 ℃.Ọ bụrụ na ejiri ya ugboro ugboro, enwere ike ịchekwa ya na 4 ℃ maka obere oge (ụbọchị iri).

We offer wonderful energy in high-quality and improve,merchandising,product sales and marketing and advertising and process for Manufacturer for Single Strip Mag-Rod Comb Nucleic Acid Test Detection disposable Consumables, Our Enterprise insists on innovation to promote the sustainable development of company, and make us become the domestic high-quality suppliers.
Onye nrụpụta makaChina Nucleic Acid na ọgwụ oriri, Na-eche ihu kpụ ọkụ n'ọnụ zuru ụwa ọnụ ahịa asọmpi, anyị na-na na ulo oru na ika ụlọ atụmatụ na emelitere mmụọ nke "mmadụ na-adabere na kwesịrị ntụkwasị obi ọrụ", na nzube iji nweta zuru ụwa ọnụ ude na-adigide mmepe.

Enweghị mgbama nkwalite

1.Taq DNA Polymerase na ngwa ahụ na-efunahụ ọrụ ya n'ihi nchekwa na-ezighị ezi ma ọ bụ njedebe nke ngwa ahụ.
Nkwanye: Kwado ọnọdụ nchekwa nke ngwa ahụ;tinyeghachi Taq DNA Polymerase kwesịrị ekwesị na sistemu PCR ma ọ bụ zụta ngwa PCR Real Time ọhụrụ maka nnwale ndị metụtara ya.

2.E nwere ọtụtụ ihe mgbochi nke Taq DNA Polymerase na DNA template.
Aro: Megharịa ndebiri ma ọ bụ belata ọnụọgụ ndebiri ejiri.

3.The Mg2 + ịta adịghị adabara.
Nkwanye: Mg2 + ntinye nke the2 × Real PCR Mix anyị na-enye bụ 3.5mM.Agbanyeghị, maka ụfọdụ primers na ndebiri pụrụ iche, ntinye Mg2+ nwere ike ịdị elu.Ya mere, ị nwere ike tinye MgCl2 ozugbo iji bulie mkpokọta Mg2+.A na-atụ aro ka ịbawanye Mg2+ 0.5mM oge ọ bụla maka njikarịcha.

4.The PCR amplification ọnọdụ adịghị adabara, na primer usoro ma ọ bụ itinye uche na-ekwesịghị ekwesị.
Aro: gosi izi ezi nke usoro nke primer ma emebiela primer;Ọ bụrụ na mgbama nkwuwapụta adịghị mma, gbalịa iwetulata okpomọkụ na-ekpo ọkụ ma mezie ntinye nke primer nke ọma.

5. Ọnụ ọgụgụ nke template dị ntakịrị ma ọ bụ nke ukwuu.
Nkwanye: Mee template linearization gradient dilution, wee họrọ mkpokọta template nwere mmetụta PCR kacha mma maka nnwale PCR oge.

NTC nwere oke fluorescence uru

1.Reagent mmetọ mere n'oge ọrụ.
Nkwanye: Dochie na ọhụrụ reagents maka ezigbo oge PCR nnwale.

2.Contamination mere n'oge nkwadebe nke usoro mmeghachi omume PCR.
Nkwanye: Were usoro nchebe dị mkpa mgbe ị na-arụ ọrụ, dị ka: iyi uwe latex, iji ntu pipette nwere nzacha, wdg.

3.The primers na-eweda n'ala, na mmebi nke primers ga-eme ka ndị na-abụghị kpọmkwem amplification.
Aro: Jiri SDS-PAGE electrophoresis chọpụta ma ọ̀tụ̀tụ̀tụ̀ ihe ndị e ji emepụta ihe na-emebi emebi, ma jiri primers ọhụrụ dochie ha maka nnwale PCR Real Time.

Primer dimer ma ọ bụ nkwalite na-abụghị nke akọwapụtara

1.The Mg2 + ịta adịghị adabara.
Nkwanye: Mg2 + ntinye uche nke 2 × Real PCR EasyTM Mix anyị na-enye bụ 3.5 mM.Agbanyeghị, maka ụfọdụ primers na ndebiri pụrụ iche, ntinye Mg2+ nwere ike ịdị elu.Ya mere, ị nwere ike tinye MgCl2 ozugbo iji bulie mkpokọta Mg2+.A na-atụ aro ka ịbawanye Mg2+ 0.5mM oge ọ bụla maka njikarịcha.

2.The PCR annealing okpomọkụ dị oke ala.
Aro: Mee ka PCR annealing okpomọkụ site 1℃ ma ọ bụ 2℃ oge ọ bụla.

3.The PCR ngwaahịa dị ogologo.
Nkwanye: Ogologo ngwaahịa PCR Real Time kwesịrị ịdị n'etiti 100-150bp, ọ bụghị karịa 500bp.

4.The primers na-eweda n'ala, na nbibi nke primers ga-eduga na ọdịdị nke kpọmkwem mmụba.
Aro: Jiri SDS-PAGE electrophoresis chọpụta ma ọ̀tụ̀tụ̀tụ̀ ihe ndị e ji emepụta ihe na-emebi emebi, ma jiri primers ọhụrụ dochie ha maka nnwale PCR Real Time.

5.The PCR usoro na-ekwesịghị ekwesị, ma ọ bụ usoro dị oke obere.
Aro: Sistemụ mmeghachi omume PCR pere mpe ga-eme ka nchọta nke ọma belata.Ọ kacha mma iji usoro mmeghachi omume nke akụrụngwa PCR ọnụọgụ tụrụ aro iji megharịa nnwale PCR Real Time.

Nkwughachi nke ụkpụrụ ọnụọgụgụ adịghị mma

1. The ngwá bụ malfunctioning.
Aro: Enwere ike inwe mperi n'etiti oghere PCR ọ bụla nke ngwaọrụ ahụ, na-ebute mmeghari na-adịghị mma n'oge njikwa okpomọkụ ma ọ bụ nchọpụta.Biko lelee dị ka ntuziaka nke ngwa kwekọrọ.

2.The sample ịdị ọcha adịghị mma.
Nkwanye: Ihe nlele na-adịghị ọcha ga-eduga na mmeghari nke nnwale ahụ na-adịghị mma, nke gụnyere ịdị ọcha nke template na primers.Ọ kacha mma imegharị ndebiri ahụ, yana SDS-PAGE na-asachapụ ihe ndị ahụ.

3.The PCR usoro nkwadebe na oge nchekwa dị ogologo.
Aro: Jiri ezigbo oge PCR usoro maka PCR nnwale ozugbo nkwadebe, na ahapụla ya n'akụkụ ruo ogologo oge.

4.The PCR amplification ọnọdụ adịghị adabara, na primer usoro ma ọ bụ itinye uche na-ekwesịghị ekwesị.
Aro: gosi izi ezi nke usoro nke primer ma emebiela primer;Ọ bụrụ na mgbama nkwuwapụta adịghị mma, gbalịa iwetulata okpomọkụ na-ekpo ọkụ ma mezie ntinye nke primer nke ọma.

5.The PCR usoro na-ekwesịghị ekwesị, ma ọ bụ usoro dị oke obere.
Aro: Sistemụ mmeghachi omume PCR pere mpe ga-eme ka nchọta nke ọma belata.Ọ kacha mma iji usoro mmeghachi omume nke akụrụngwa PCR ọnụọgụ tụrụ aro iji megharịa nnwale PCR Real Time.