Ngwakọta ụlọ ọrụ 2 X ngwa ngwa Pfus PCR Master Mix na Pfu DNA Polymerase Dntps PCR Buffer
Anyị na-emesi ọganihu ma na-ewebata ngwaahịa ọhụrụ n'ime ahịa kwa afọ maka ụlọ ọrụ mmepụta ihe 2 X Fast Pfus PCR Master Mix with Pfu DNA Polymerase Dntps PCR Buffer, Ka anyị na-aga n'ihu, anyị na-ejide anya na ngwaahịa ngwaahịa anyị na-ebuwanye ibu ma na-emeziwanye ụlọ ọrụ anyị.
Anyị na-emesi ọganihu na iwebata ngwaahịa ọhụrụ n'ime ahịa kwa afọ makaChina PCR Master Mix na PCR Reagent, Anyị na-achọ ịghọ ụlọ ọrụ ọgbara ọhụrụ na ezigbo azụmahịa nke "Ịdị ọcha na ntụkwasị obi" yana ebumnuche nke "inye ndị ahịa ọrụ kachasị mma na ngwaahịa kachasị mma".Anyị ji ezi obi na-arịọ maka nkwado gị na-agbanweghị agbanwe ma nwee ekele maka ndụmọdụ na nduzi gị dị mma.
Nkọwapụta
50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns
Ngwa ngwa
Akụkụ I | Ihe nchekwa PS1 |
Ihe nchekwa P2 | |
6 × DNA na-ebufe ihe nchekwa | |
Akụkụ II | Ihe nchekwa PS2 |
2× Akwukwo PCR MfeTMNgwakọta |
Atụmatụ & uru
∎ Ọ dịghị ọcha DNA na-ewe oge yana ọnụ
■ Ihe dị obere
∎ Mfe - enwere ike idobe ihe nlele ahụ na mmeghachi omume PCR ma ọ bụ mmeghachi omume lysis na-egbutughị ma ọ bụ egweri
∎ 2 × Mix, na-ebelata mperi mperi ihe nlele na oge nkwadebe usoro mmeghachi omume
■ Enwere ike ịmechaa nkwadebe ngwa ngwa n'ime nkeji 10, enwere ike mechaa mmeghachi omume PCR n'ime opekata mpe nkeji iri ise.
∎ Enwere ike mechaa mmeghachi omume nnabata dị elu na efere PCR 96 nke ọma
Ngwa ngwa
Ngwa: njirimara nke osisi transgenic, genotyping, wdg.
Ihe atụ: akwụkwọ osisi bara ụba na polysaccharide ma ọ bụ polyphenol
Nlereanya nha: dayameta 2-3mm (usoro kpọmkwem), dayameta 5-7mm (usoro lysis)
Oke nchọpụta: PCR ngwaahịa ≤1kb
Usoro ọrụ
Electropherogram
Anyị na-emesi ọganihu ma na-ewebata ngwaahịa ọhụrụ n'ime ahịa kwa afọ maka ụlọ ọrụ mmepụta ihe 2 X Fast Pfus PCR Master Mix with Pfu DNA Polymerase Dntps PCR Buffer, Ka anyị na-aga n'ihu, anyị na-ejide anya na ngwaahịa ngwaahịa anyị na-ebuwanye ibu ma na-emeziwanye ụlọ ọrụ anyị.
Nkwanye ụlọ ọrụChina PCR Master Mix na PCR Reagent, Anyị na-achọ ịghọ ụlọ ọrụ ọgbara ọhụrụ na ezigbo azụmahịa nke "Ịdị ọcha na ntụkwasị obi" yana ebumnuche nke "inye ndị ahịa ọrụ kachasị mma na ngwaahịa kachasị mma".Anyị ji ezi obi na-arịọ maka nkwado gị na-agbanweghị agbanwe ma nwee ekele maka ndụmọdụ na nduzi gị dị mma.