A na-ebutekarị oke ala nke A260/A230 site na adịghị ọcha nwere ogologo ogologo mmịnye kacha na 230nm.Ka anyị hụ ihe adịghị ọcha ndị a gụnyere:

• Ihe mmetọ nkịtị	Ogologo ife ofufe	Mmetụta oke
  Protein	~ 230nm na 280nm	Mbelata nke A260/A 280na A260/A 280oke
  Nnu guanidine	220-240 nm	Belata A260/A 280oke
  Phenol	~ 270nm	-
  Trizol	~ 230nm na 270nm	Belata A260/A 280oke
  EDTA	-230 nm	Belata A260/A 280oke
  Ethanol	230-240 nm	Belata A260/A 280oke

 
 
 
Ogologo ogologo mmịnye na uru dị iche iche nke mmetọ nkịtị

Pọrịa roten
Enwere ike were mmetọ protein dị ka mmetọ kachasị na usoro mmịpụta nucleic acid.Protein dị n'etiti akụkụ mmiri dị elu na nke alaorganicnkeji oge .Mmetọ ga-ebelata oke nke A260/A280 na A260/A230 n'otu oge ahụ, na nha nke A260/A230 ga-agbanwe nke ọma karịa nha nke A260/A280.
N'oge sochirinụntụgharị ntụgharịor mmeghachi omume qPCR, Mgbochi protein nwere ike igbochi ma ọ bụ gbochie ọrụ enzyme.Ụzọ kachasị mma isi zere mmetọ protein bụ iburu n'uche ụkpụrụ nke "kama karịa karịa, obere ego ọtụtụ ugboro" mgbe ị na-achọ ihe dị elu.

2. Guanidinium mmetọ
hydrochloride (GuHCl) na guanidine thiocyanate (GTC) nwere mmetụta nke denaturing protein, nke nwere ike ibibi membranes cell ngwa ngwa n'oge usoro mmịpụta nucleic acid, na-eme ka protein denaturation na mmiri ozuzo.Ogologo ogologo mmịpụta nke GuHCl na GTC dị n'etiti 220-240 nm, yanaNnu guanidinium fọdụrụnụ ga-ebelata oke nke A260/A230.Ọ bụ ezie na nnu guanidinium fọdụrụnụ ga-ebelata oke,mmetụta na downstream nnwale bụ n'ezie negligible.

3. Trizol mmetọ
Akụkụ bụ isi nke Trizol bụ phenol.Isi ọrụ nke phenol bụ lyse mkpụrụ ndụ ma hapụ protein na ihe nucleic acid n'ime sel.Ọ bụ ezie na phenol nwere ike imebi protein nke ọma, ọ nweghị ike igbochi ọrụ RNase kpamkpam.Ya mere, 8 -hydroxyquinoline, guanidine isothiocyanate, β-mercaptoethanol, wdg na-agbakwunye na TRIzol iji gbochie endogenous na exogenous RNase.
Mgbe ị na-ewepụta RNA cellular, Trizol nwere ike lyse sel ngwa ngwa ma gbochie nuclease ewepụtara na sel, na ihe fọdụrụnụ Trizol ga-ebelata oke nke A260/A230.
Usoro nhazi: Mgbe centrifuging, ọ ga-kwuru na phenol na Trizol dị mfe soluble na mmiri na-adọ n'okpuru ọnọdụ nke 4 Celsius na ụlọ okpomọkụ.

4. Ethanol fọdụrụnụ
A na-eji ethanol mee ihe na usoro ikpeazụ iji mee ka DNA pụta mgbe ọ na-agbaze ion nnu nke nwere ike jikọta na DNA.The absorption wavelength nke kasị eluabsorption kacha nkeethanol dịkwa na 230-240 nm, nkega-ebelata oke nke A260/A230.
Enwere ike ịmegharị usoro iji zere ihe fọdụrụ ethanol ugboro abụọ n'oge elution ikpeazụ, na-afụ namkpuchi uzuzumaka nkeji abụọ iji kwe ka ethanol pụta n'ụzọ zuru ezu tupu ị gbakwunye ihe nchekwa maka elution.
Agbanyeghị, ekwesịrị ịmara na oke ahụ bụ naanị ntụle nyocha nke ogo RNA.Ọ bụrụ na a na-ahazi ọrụ ndị ahụ a kpọtụrụ aha n'elu, ngbanwe dị n'etiti oke na ọkọlọtọ agaghị enwe mmetụta dị ukwuu na nyocha nke ala.
Ngwaahịa ndị emetụtara:
Ngwakọta RNA dị iche iche anụmanụ
Ngwakọta mkpokọta RNA kewapụ ihe ọkụkụ
Ngwakọta mkpokọta cell RNA dịpụrụ adịpụ
Ngwakọta mkpokọta RNA kewapụrụ ihe ọkụkụ Plus