Ngwakọta mkpokọta RNA Isolation Plus
Nkọwa ngwa:
Wepu DNA nke ọma site na iji kọlụm DNA- Cleaning
Wepu DNA na-agbakwunyeghị DNAse
Mfe-arụ ọrụ niile na-arụcha na ụlọ okpomọkụ
Ngwa ngwa-enwere ike ịrụcha ọrụ n'ime nkeji iri atọ
Safe — ọ dịghị organic reagent eji
Nkọwapụta
Nkwadebe 50, 200 nkwadebe
Ngwa ahụ na-eji kọlụm ogho na usoro nke Foregene mepụtara, nke nwere ike wepụta RNA dị ọcha na ịdị elu nke ọma site na anụ ahụ osisi dị iche iche nwere nnukwu polysaccharides ma ọ bụ polyphenols.Ọ na-enye kọlụm DNA- Cleaning nke nwere ike wepu DNA genomic ngwa ngwa site na lysate na anụ ahụ.Kọlụm RNA naanị nwere ike jikọta RNA nke ọma.Ngwa ahụ nwere ike ịhazi ọnụ ọgụgụ dị ukwuu nke ihe nlele n'otu oge.
Sistemu niile enweghị RNase, yabụ na RNA emechara adịghị eme ka ọ dị ala.Buffer PRW1 na Buffer PRW2 nwere ike hụ na RNA enwetaghị protein, DNA, ion, na ogige organic emerughi ya.
Ngwa ngwa
| Buffer PSL1, ihe nchekwa PS, ihe nchekwa PSL2 |
| Ihe nchekwa PRW1, ihe nchekwa PRW2 |
| ddH na-efu RNase2O, kọlụm na-ehicha DNA |
| RNA-naanị kọlụm |
Atụmatụ & uru
■ Arụ ọrụ na ụlọ okpomọkụ (15-25 ℃) n'ime usoro dum, na-enweghị ice bat na obere okpomọkụ centrifugation.
∎ Ngwa RNase anaghị akwụ ụgwọ zuru oke, ọ nweghị mkpa ichegbu onwe gị maka mmebi RNA.
■ Kwesịrị ekwesị maka ịsachapụ RNA site na ihe atụ osisi nke polysaccharides na polyphenols.
∎ kọlụm DNA- Cleaning na-ejikọta kpọmkwem na DNA, ka ngwa ahụ wee nwee ike wepụ nje DNA nje na-agbakwunyeghị DNA.
∎ Mkpụrụ RNA dị elu: Kọlụm naanị RNA nwere ike sachaa RNA nke ọma.
∎ Ọsọ ọsọ: dị mfe iji rụọ ọrụ ma nwee ike mechaa n'ime nkeji iri atọ.
■ Nchekwa: ọ dịghị organic reagenti a chọrọ.
■ Ọdịmma dị elu: Iberibe RNA ndị a sachara dị ọcha, enweghị protein na adịghị ọcha ndị ọzọ, ma nwee ike zute ngwa nnwale dị iche iche nke ala.
Ngwa ngwaahịa
∎ Ngwa mgbada ala: njikọ cDNA nke mbụ, RT-PCR, cloning molecular, Northern Blot, wdg.
■ Ihe nlele: anụ ahụ osisi dị ọhụrụ ma ọ bụ oyi kpọnwụrụ nke polysaccharides na polyphenols
■ Usoro onunu ogwu: 50mg osisi anụ ahụ
∎ Ike RNA kacha mma nke kọlụm nchacha: 80 μg
■ Olu olu: 50-200 μl
Ngwa ngwa
Ọ dabara maka mmịpụta na ịdị ọcha nke mkpokọta RNA site na nlele anụ ahụ dị ọhụrụ ma ọ bụ oyi kpọnwụrụ (karịsịa anụ ahụ ahịhịa ọhụrụ) nwere nnukwu polysaccharide na ọdịnaya polyphenol.
Usoro ọrụ
Eserese
Plant Total RNA Isolation Kit Plus na-ahazi 50mg nke akwụkwọ ọhụrụ nke polysaccharides na polyphenols, na 5% RNA dị ọcha ka ejiri electrophoresis nwalee.
1: banana
2: Ginkgo
3: owu
4: pọmigranet
Nchekwa na Shelf ndụ
Enwere ike ịchekwa ngwa a maka ọnwa 24 n'okpuru ọnọdụ akọrọ na ụlọ okpomọkụ (15-25 ℃);Ọ bụrụ na ọ dị mkpa ka ịchekwa ya ogologo oge, enwere ike ịchekwa ya na 2-8 ℃.
Enwere ike itinye PSL1 buffer na 4 ℃ maka ọnwa 1 mgbe agbakwunyere β-mercaptoethanol (a na-atụ aro ka ịgbakwunye ya n'otu oge nnwale).
