Ngwa Ngwa Mwepụta Mwepu nke Ala DNA & Reagents maka DNA Ala
Nkọwa ngwa:
Mee ka ọ dị ọcha ngwa ngwa wee nweta DNA genomic dị elu site na ụdị microorganisms dị iche iche nke ala.
◮Enweghị mmetọ RNase:Ogidi DNA-naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepụ RNA na DNA genomic na-agbakwunyeghị RNase n'oge nnwale ahụ, na-egbochi ụlọ nyocha ahụ site na RNase exogenous metọọ.
◮Ọsọ ọsọ:Foregene Protease nwere ọrụ dị elu karịa proteases yiri ya, ma na-agbari ihe nlele anụ ahụ ngwa ngwa;arụ ọrụ ahụ dị mfe, yana ọrụ mmịpụta DNA nke genomic nwere ike mechaa n'ime nkeji 20-80.
◮Dị mma:A na-eme centrifugation na okpomọkụ nke ụlọ, ọ dịghịkwa mkpa maka 4 Celsius C obere okpomọkụ centrifugation ma ọ bụ ethanol precipitation nke DNA.
◮Nchekwa:Ọ dịghị organic reagent mmịpụta a chọrọ.
◮Ịdị mma Dị elu:DNA genomic nke ewepụtara nwere nnukwu iberibe, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe nyocha dị iche iche chọrọ.
◮Sistemụ Micro-elution:Ọ nwere ike ịbawanye ntinye nke DNA genomic, nke dị mma maka nchọpụta ala ma ọ bụ nnwale.
Nkọwa
Ngwa a na-enye ụzọ dị ngwa ma dị mfe maka ịwepụta DNA genomic site na isi mmalite dị iche iche nke ala.Enwere ọnụ ọgụgụ buru ibu nke ihe mgbochi dị ka humic acid na ion metal na nlele ala.Ọbụlagodi na ihe ndị a dị na ntakịrị ntakịrị na DNA dị ọcha, ha ga-enwe mmetụta na mmeghachi omume ala, dị ka PCR na mgbochi enzyme mgbaze.Ya mere, isi ihe na-eme ka DNA ala dị ọcha bụ otu esi ewepụ ihe mgbochi na ala nke ọma.
Ngwa a na-eji DNA-Only Kọlụm nwere ike jikọọ kpọmkwem na DNA, ihe ọhụrụ Foregene Protease na sistemu nchekwa pụrụ iche, nke nwere ike wepu ihe mgbochi dị iche iche na ala na-enweghị mmịpụta ihe mgbaze organic ma ọ bụ ethanol na oke mmiri isopropanol.Enwere ike ịmechaa mmịpụta DNA site na nlele ala n'ime nkeji iri itoolu.
Nkọwapụta
Nkwadebe 50, nkwadebe 100, nkwadebe 250
Ngwa ngwa
| Ihe nchekwa SG1 |
| Ihe nchekwa SG2 |
| Ihe nchekwa SG3 |
| Ihe nchekwa SG4 |
| Ihe nchekwa PW |
| Ihe nchekwa WB |
| Ihe nchekwa EB |
| Buffer TE |
| Protease nke mbụ |
| DNA-naanị kọlụm |
| Ntuziaka |
Atụmatụ & uru
-Ọnweghị mmetọ RNase: Otu kọlụm DNA-naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepu RNA na DNA genomic na-agbakwunyeghị RNase n'oge nnwale ahụ, na-ezere ụlọ nyocha ahụ ka ọ ghara imerụ ya site na RNase dịpụrụ adịpụ.
-Ọsọ ọsọ ọsọ: Ọrụ ahụ dị mfe, yana ọrụ mmịpụta DNA nke ala genomic nwere ike mechaa n'ime nkeji 90.
-dị mma: A na-eme centrifugation na ụlọ okpomọkụ, ọ dịghịkwa mkpa maka 4°C centrifugation dị ala ma ọ bụ ethanol ọdịda DNA.
-Safety: Ọ dịghị organic reagent mmịpụta a chọrọ.
-Ọdịmma dị elu: DNA genomic amịpụtara nwere nnukwu iberibe, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe achọrọ nnwale dị iche iche.
Ngwa ngwa
Ọ dabara maka ịsacha DNA genomic site na ihe atụ ndị a: ihe nlele silt, ala akwa ifuru, ala na-azọ ụkwụ, ala ọhịa, ala ahịhịa, ala ọdọ mmiri, ala ọdọ mmiri, ala ahịhịa na ihe atụ ndị ọzọ.
Usoro ọrụ
Nchekwa na Shelf ndụ
-Enwere ike ịchekwa ngwa a maka ọnwa 12 n'okpuru ọnọdụ akọrọ na ụlọ okpomọkụ (15-25 ° C), ọ bụrụ na ọ dị mkpa ka echekwara ya ogologo oge, enwere ike ịchekwa ya na 2-8 ° C.
Rịba ama: Ọ bụrụ na echekwara ya na obere okpomọkụ, ngwọta na-adịkarị mfe mmiri ozuzo.Tupu ojiji, jide n'aka na ị ga-etinye ihe ngwọta na kit na ụlọ okpomọkụ maka oge.Ọ bụrụ na ọ dị mkpa, kpoo ya na mmiri ịsa mmiri 37 Celsius C maka nkeji 10 iji gbazee mkpọda ahụ, ma gwakọta ya tupu ejiri ya.
-Foregene Protease ngwọta nwere usoro pụrụ iche, nke na-arụ ọrụ mgbe echekwara na ụlọ okpomọkụ ruo ogologo oge (ọnwa 3);ọrụ ya na nkwụsi ike ya ga-aka mma ma ọ bụrụ na echekwara ya na 4 Celsius C, ya mere, a na-atụ aro ka ịchekwa ya na 4 Celsius C, cheta ka ọ ghara idobe ya na -20 Celsius.
-A na-echekwa ntụ ntụ Lysozyme akọrọ na -20 Celsius;A na-ekewa ngwọta Lysozyme a kwadebere n'ime obere akụkụ ma chekwaa ya na -20 Celsius.
