Kit DNA Mwepu FFPE DNA si na Sample FFPE
Nkọwa ngwa:
Mee ngwa ngwa DNA genomic dị elu site na anụ ahụ ndị etinyere na paraffin dị ka akụkụ paraffin na paraffin blocks.
◮Enweghị mmetọ RNase:Ogidi DNA-naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepụ RNA na DNA genomic na-agbakwunyeghị RNase n'oge nnwale ahụ, na-egbochi ụlọ nyocha ahụ site na RNase exogenous metọọ.
◮Ọsọ ọsọ:Foregene Protease nwere ọrụ dị elu karịa proteases yiri ya, ma na-agbari ihe nlele anụ ahụ ngwa ngwa;arụ ọrụ ahụ dị mfe, yana ọrụ mmịpụta DNA nke genomic nwere ike mechaa n'ime nkeji 20-80.
◮Dị mma:A na-eme centrifugation na okpomọkụ nke ụlọ, ọ dịghịkwa mkpa maka 4 Celsius C obere okpomọkụ centrifugation ma ọ bụ ethanol precipitation nke DNA.
◮Nchekwa:Ọ dịghị organic reagent mmịpụta a chọrọ.
◮Ịdị mma Dị elu:DNA genomic nke ewepụtara nwere nnukwu iberibe, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe nyocha dị iche iche chọrọ.
◮Sistemụ Micro-elution:Ọ nwere ike ịbawanye ntinye nke DNA genomic, nke dị mma maka nchọpụta ala ma ọ bụ nnwale.
Nkọwa
A na-arara ngwa a iji wepụta na sachaa DNA genomic dị elu site na anụ ahụ edoziri formaldehyde ma ọ bụ anụ ahụ etinyere paraffin.
A ga-emebi DNA genomic nke ihe atụ nke paraffin n'ihi oge nchekwa ma ọ bụ gburugburu ebe obibi, na-eme ka o sie ike wepụ DNA genomic.Ngwa a na-anakwere usoro kachasị mma pụrụ iche, nke dabara maka ịwepụta DNA genomic site na ihe nlere agbakwunyere na paraffin nwere nnukwu ihe ịga nke ọma nke mmịpụta.Usoro niile adịghị mkpa iji phenol na-egbu egbu, chloroform ma ọ bụ mmiri ozuzo isopropanol.
Ngwa a na-eji kọlụm ntugharị nke nwere ike jikọta DNA kpọmkwem, ihe ọhụrụ Foregene Protease Plus, yana sistemụ nchekwa pụrụ iche, nke nwere ike wepụ DNA genomic anụ ahụ dị elu nke nwere paraffin n'ime awa 2-5.
Nkọwapụta
Nkwadebe 50, nkwadebe 100, nkwadebe 250
Ngwa ngwa
| Ihe nchekwa FL1 |
| Ihe nchekwa FL2* |
| Ihe nchekwa PW* |
| Ihe nchekwa WB |
| Ihe nchekwa EB |
| Foregene Protease Plus |
| DNA-naanị kọlụm |
| Ntuziaka |
Atụmatụ & uru
-Ọnweghị mmetọ RNase: Otu kọlụm DNA-naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepu RNA na DNA genomic na-agbakwunyeghị RNase n'oge nnwale ahụ, na-ezere ụlọ nyocha ahụ ka ọ ghara imerụ ya site na RNase dịpụrụ adịpụ.
-Ọsọ ọsọ ọsọ: Foregene Protease Plus nwere ọrụ dị elu karịa proteases ndị yiri ya, ọ na-agbarikwa sample anụ ahụ ngwa ngwa;ọrụ ahụ dị mfe, yana ọrụ mmịpụta DNA nke genomic nwere ike mechaa n'ime awa 2-5.
-dị mma: A na-eme centrifugation na ụlọ okpomọkụ, ọ dịghịkwa mkpa 4°C centrifugation dị ala ma ọ bụ ethanol ọdịda DNA.
-Safety: ọ dịghị organic reagent mmịpụta a chọrọ.
-Ego dị elu: Iberibe DNA genomic amịpụtara buru ibu, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe achọrọ nnwale dị iche iche.
Ngwa ngwa
Ọ dabara maka mmịpụta na ịdị ọcha nke DNA genomic site na ihe nleba anya nke formaldehyde na anụ ahụ etinyere paraffin.
Usoro ọrụ
Nchekwa na Shelf ndụ
-Enwere ike ịchekwa ngwa a maka ọnwa 12 n'okpuru ọnọdụ akọrọ na ụlọ okpomọkụ (15-25 ° C);Ọ bụrụ na achọrọ ịchekwa ya ogologo oge, enwere ike ịchekwa ya na 2-8 Celsius.
Rịba ama: Ọ bụrụ na echekwara ya na obere okpomọkụ, ngwọta na-adịkarị mfe mmiri ozuzo.Tupu iji ya, jide n'aka na ị ga-etinye ngwọta na kit na ụlọ okpomọkụ ruo oge ụfọdụ.Ọ bụrụ na ọ dị mkpa, kpoo ya na mmiri ịsa mmiri 37 Celsius C maka nkeji 10 iji gbazee mkpọda ahụ, ma gwakọta ya tupu ejiri ya.
-Foregene Protease Plus ngwọta nwere usoro pụrụ iche nke na-arụ ọrụ mgbe echekwara na ụlọ okpomọkụ ogologo oge (ọnwa 3);ọ na-arụsi ọrụ ike ma kwụsie ike mgbe a na-echekwa ya na 4 Celsius C, ya mere a na-atụ aro ka ịchekwa ya na 4 Celsius C, cheta ka ọ ghara idobe ya na -20 Celsius.
