Ngwa EndoFree Maxi Plasmid (Ogidi Ogwu)
Nkọwa ngwa:
Usoro mmịpụta dum na-ewe naanị awa 1, na-eme ka ọrụ dị mfe na ngwa ngwa.
Nkọwa
EndoFree Maxi Plasmid Kit na-anakwere usoro mgbasa ozi silica akpụkpọ anụ pụrụ iche iji kesaa DNA plasmid nke ọma na nke ọma.Site na ijikọ pụrụ iche Endotoxin Removal Buffer P4 na EndoFree Maxi Filtration Columns, endotoxin, proteins na ndị ọzọ adịghị ọcha nwere ike iwepụ nke ọma.Usoro mmịpụta dum na-ewe naanị awa 1, na-eme ka ọrụ dị mfe na ngwa ngwa.
Ọnụ ego nje bacteria akwadoro maka omume ọ bụla: Maka plasmid dị elu, a na-atụ aro ka iji 100 ml nke mgbasa ozi omenala nje bacteria, na mkpụrụ sitere na ya na-adịkarị gburugburu 500-1500 μg.Maka plasmid dị obere, a na-atụ aro mgbasa ozi omenala nje bacteria 200 ml ka imepụta 200-600 μg plasmids.
Ngwa ngwa
| Ngwaahịa Ngwa | D909-E(10 nkeji) |
| Ihe nchekwa BL | 30 ml |
| Ihe nchekwa P1 | 100 ml |
| Ihe nchekwa P2 | 100 ml |
| Ihe nchekwa P4 | 100 ml |
| Ihe nchekwa DW2 | 44 ml |
| Ihe nchekwa EB | 30 ml |
| RNase A (10 mg / ml) | 1 ml |
| Ogidi ogidi DNAPure Maxi | 10 pcs |
| FinePure Maxi Filter sirinji | 10 pcs |
| 50 ml mkpokọta tubes | 10 pcs |
Nchọpụta nke Plasmid DNA Concentration na Ịdị Ọcha
Enwere ike ịchọpụta ịdị ọcha na ntinye nke DNA plasmid ewepụtara site na agarose gel electrophoresis na ultraviolet spectrophotometer.Otu uru OD260 nke 1 dabara na ihe dịka 50 μg/ml nke DNA nwere eriri abụọ.Uru OD260/OD280 nke DNA plasmid a sachara na-adịkarị n'ogo nke 1.7-1.9.Enwere ike itinye DNA plasmid dị ọcha ozugbo na nnwale nwere oke ịdị ọcha chọrọ, dị ka nnyefe cell ma ọ bụ nnwale vivo.
Ngwa ngwa
DNA plasmid ewepụtara site na ngwa a nwere ike itinye ya na arụ ọrụ dị iche iche, gụnyere mgbaze enzymatic, PCR, sequencing, ligation, mgbanwe yana ntụgharị maka ụdị sel dị iche iche.
Nchekwa na Shelf ndụ
Enwere ike ịchekwa ngwa EndoFree Maxi Plasmid na ụlọ okpomọkụ (15-25°C) n'okpuru ọnọdụ akọrọ ruo ọnwa 12.Tinye RNase A na nchekwa P1 tupu ojiji mbụ, gwakọta nke ọma ma chekwaa na 2-8°C.Enwere ike ịchekwa ngwakọta ahụ nke ọma karịa ọnwa 6.
