usoro ngwa ngwa maka ikewapụ na ịdị ọcha nke mkpokọta RNA site na sel
Anyị nwere obi ụtọ maka afọ ojuju onye zụrụ gị na nnabata zuru oke n'ihi nchụso anyị na-aga n'ihu nke ịdị elu ma dokwara na ngwọta na ọrụ maka usoro ngwa ngwa maka ikewapụ na ịdị ọcha nke mkpokọta RNA site na sel, na-agbaso obere nkà ihe ọmụma azụmaahịa nke 'onye ahịa 1st, ga-aga n'ihu', anyị ji ezi obi na-anabata ndị ahịa si n'ụlọ gị ma soro anyị na-arụkọ ọrụ na mba ofesi.
Anyị nwere obi ụtọ maka afọ ojuju onye zụrụ gị na nnabata zuru oke n'ihi nchụso anyị na-aga n'ihu nke ịdị elu dị elu ma n'otu aka ahụ na ngwọta na ọrụ makausoro ngwa ngwa maka ikewapụ na ịdị ọcha nke mkpokọta RNA site na sel ndị mepere emepe, Anyị ụlọ ọrụ na-arụ ọrụ ụkpụrụ nke "iguzosi ike n'ezi dabeere, imekọ ihe ọnụ kere, ndị mmadụ gbakwasara, imeri-mmeri imekọ ihe ọnụ".Anyị na-atụ anya na anyị na onye ọchụnta ego si n'akụkụ ụwa niile nwere ike inwe mmekọrịta enyi na enyi.
Nkọwapụta
Nkwadebe 50, 200 Preps
Ngwa ngwa
| Ihe nchekwa cRL1, ihe nchekwa cRL2 |
| Ihe nchekwa RW1, ihe nchekwa RW2 |
| ddH na-efu RNase2O, kọlụm na-ehicha DNA |
| RNA-naanị kọlụm |
Atụmatụ & uru
■ A na-arụ ọrụ ahụ dum na ụlọ okpomọkụ (15-25 ℃), na-enweghị ice bat na obere okpomọkụ centrifugation.
■ Ngwa niile enweghị RNase, ọ nweghị mkpa ichegbu onwe gị maka mmebi RNA.
∎ kọlụm DNA- Cleaning na-ejikọta DNA kpọmkwem, ka ngwa ahụ wee nwee ike wepụ nje DNA nje na-etinyeghị DNA ọzọ.
∎ Mkpụrụ RNA dị elu: Kọlụm naanị RNA nwere ike sachaa RNA nke ọma.
∎ Ọsọ ọsọ: dị mfe iji rụọ ọrụ ma nwee ike mechaa n'ime nkeji iri na otu.
■ Nchekwa: Ọ dịghị ihe reagent organic achọghị.
■ Ọdịmma dị elu: RNA dị ọcha dị oke ọcha, enweghị protein na adịghị ọcha ndị ọzọ, ọ nwekwara ike izute nnwale dị iche iche na-esote.
Ngwa ngwa
Ọ dabara maka mmịpụta na ịsacha mkpokọta RNA site na mkpụrụ ndụ emebere na efere 96, 24, 12 na 6.
Usoro ọrụ
Eserese
Eserese batrị agarose gel nke Cell Total RNA Isolation Kit na-emeso ọnụọgụ mkpụrụ ndụ dị iche iche dị n'elu, elu 20μl, were 2μl dị ọcha RNA 1%.
Nchekwa na ndụ nchekwa
Enwere ike ịchekwa ngwa ahụ maka ọnwa 12 na okpomọkụ nke ụlọ (15-25 ℃) na 2-8 ℃ maka ogologo oge (ọnwa 24).
Buffer cRL1 nwere ike ịchekwa na 4 ℃ maka 1 ọnwa mgbe agbakwunyere 2-hydroxy-1-ethanethiol(nhọrọ) .We've been proud of your substantial purchaser satifaction and wide acceptance due to our persistent pursuit of high quality both equally on solution and service for Short Lead Time for China 2 Years Warranty Industrial Coating the Small Business Coating, Spdheray 1. , forge ahead', anyị ji ezi obi na-anabata ndị ahịa si n'ụlọ gị na esenidụt ka anyị na ha na-arụkọ ọrụ.
Short Lead Time for China Powder Coating Booth, Industrial mkpuchi, Anyị ụlọ ọrụ na-arụ ọrụ site na ọrụ ụkpụrụ nke "iguzosi ike n'ezi dabeere, imekọ ihe ọnụ kere, ndị mmadụ gbakwasara, imeri-mmeri imekọ ihe ọnụ".Anyị na-atụ anya na anyị na onye ọchụnta ego si n'akụkụ ụwa niile nwere ike inwe mmekọrịta enyi na enyi.
