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Anụ Ahụ Na-eduzi PCR kit-UNG
Ngwa a na-eji sistemu nchekwa lysis pụrụ iche iji wepụta DNA ngwa ngwa site na ntanetị anụ ahụ maka mmeghachi omume PCR, yabụ ọ dabara adaba maka nnwale mkpụrụ ndụ buru ibu.
Usoro nke ịhapụ DNA genomic site na nchekwa lysis ka emechara n'ime nkeji 10-30 na 65°C. Enweghị usoro ndị ọzọ dị ka protein na mwepụ RNA achọrọ, a ga-ejikwa DNA nke wepụtara dị ka ndebiri maka mmeghachi omume PCR.
2×PCR mfeNwachukwu Mix (UNG) jiri dUTP kama dTTP na ndabere nke 2×PCR mfeNwachukwu Gwakọta, ma gbakwunye enzyme UNG (Uracil-N-glycosylase) nke nwere ike iweda ndebiri nwere dUTP n'otu oge. Tupu mmeghachi omume PCR, a na-eji enzyme UNG mebie ngwaahịa PCR nwere uracil. UNG enzyme agaghị enwe mmetụta ọ bụla na ndebiri nke na-enweghị uracil, si otú ahụ na-achọpụta kpọmkwem na njupụta nke mmụba ma na-egbochi ohere nke PCR ngwaahịa mmetọ n'oge nnukwu mkpụrụ ndụ ihe nketa.
D-Taq DNA polymerase bụ DNA polymerase nke mepụtara nke Foregene maka mmeghachi omume PCR kpọmkwem. D-Taq DNA polymerase nwere nnagide siri ike nye ọtụtụ ndị mmeghachi omume PCR, ma nwee ike ịkọwapụta DNA dị iche iche n'ụzọ dị iche iche na usoro mmeghachi omume dị mgbagwoju anya, yana mmụbawanye ngwa ngwa nwere ike iru 2Kb / min, nke dabara adaba maka mmezi Direct PCR.
