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Ahịhịa Zebra Kwụsị Kit PCR
Ngwa a na-eji sistemu nchekwa lysis pụrụ iche iji wepụta DNA genomic ngwa ngwa site na zebrafish na anụ ndị ọzọ dị na azụ, nku ọdụ ma ọ bụ akwa akwa azụ maka mmeghachi omume PCR, yabụ ọ dabara adaba maka nnwale mkpụrụ ndụ buru ibu. Usoro nke ịhapụ DNA genomic site na nchekwa lysis ka emechara n'ime 10-30 nkeji na 65 Celsius. Enweghị usoro ndị ọzọ dị ka protein na mwepụ RNA achọrọ, na DNA nwere ike iji dị ka ndebiri maka mmeghachi omume PCR.
2, PCR Easy TM Mix nwere nnagide siri ike nye ndị mmeghachi omume PCR, ma nwee ike iji lysate nke nlele ahụ nwalee ya dị ka ndebiri maka mmụba nke ọma na nke ọma. Ihe nyocha a nwere ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, nchekwa nchekwa, PCR optimizer na stabilizer. Ejiri ya na nchekwa lysis nwere ike ịchọpụta ngwa ngwa na ngwa ngwa ọ nwere njirimara nke nwere nnukwu uche, nkọwa siri ike, na ezi nkwụsi ike.
D-Taq DNA polymerase bụ DNA polymerase nke mepụtara nke Foregene maka mmeghachi omume PCR kpọmkwem. D-Taq DNA polymerase nwere nnagide siri ike nye ọtụtụ ndị mmeghachi omume PCR, ma nwee ike ịkọwapụta DNA dị iche iche n'ụzọ dị iche iche na usoro mmeghachi omume dị mgbagwoju anya, yana mmụbawanye ngwa ngwa nwere ike iru 2Kb / min, nke dabara adaba maka mmezi Direct PCR.
