Kedu ihe bụ PCR Direct

PCR Direct bụ usoro nke mmụba DNA ozugbo site naọbara, anụ ahụ anụ ahụ,oke oke,cell, nti oke,zebra azụ, akwa azụ,akwụkwọ osisi,mkpụrụma ọ bụ ihe nlele anụ ahụ mbụ dị ndụ ndị ọzọ na-enweghị ime ikewapụ DNA na usoro nchacha.Usoro PCR ozugbo nwere ike belata oge nnwale, yana ọnụ ahịa genotyping na nnukwu ọrụ.Ọ na-enyekwa nhọrọ ka mma ma ọ bụrụ na ọ na-eche ihe ịma aka ndị dị n'ịkwalite obere ihe nlele ebe nzọụkwụ dị ọcha nwere ike bute ọnwụ nlele.

Ngwakọta FOREGENE's PCR nwere nguzogide nrụgide siri ike, n'ihi ike nke FOREGENE ike patented Taq enzyme (patent nyere ikike: ZL20161034512.0 (China), EP3450560 (Europe), PCT/CN2017/082154 (US), patent 6894926 (Japan)).Enzyme Taq ya na-eji teknụzụ recombination DNA iji jikọta mpaghara nhazi njikọ DNA nke protein nucleic acid nke archaeal na Taq DNA polymerase na-eguzogide okpomọkụ iji wuo DNA ndebiri nwere mmekọrịta dị ukwuu.Taq mmalite na-ekpo ọkụ ka mma nke na-ejigide ọrụ dị iche iche nke DNA polymerase mbụ. Nke a Enzyme Taq nwere ike jikọta na DNA DNA ma malite njikọ DNA na ndebiri ntinye uche dị ala na gburugburu mgbagwoju anya.

For ọmụmaatụ,itdị mfe dị ka ịgbakwunye gịcell,osisi (obere akwukwo, mgbọrọgwụ ma ọ bụ mkpụrụ) ma ọ bụ anụmanụ sample kaihe nchekwa, na-agbakwunye pụrụ ichenna ukwu mix nakpọmkwemprimers na PCR tubes na-agba ọsọ ya site a thermal cycler.Kit arụmọrụ dị mma maka nyocha nlele buru ibu ebe ịchekwa oge dị oke mkpa.

A na-ahọrọ sistemu mmeghachi omume PCR nwere UNG enzyme na dUTP dịka nnwale chọrọ, nke nwere ike zere mmetọ nke ngwaahịa nkwalite PCR kpatara.