Ngwa nje DNA/RNA (Mkpụrụ osisi magnetik)
Nkọwa ngwa:
Teknụzụ magnetik pụrụ iche na-enyere aka ịsacha acid nucleic dị elu nke enweghị protein, nucleases na adịghị ọcha ndị ọzọ.
Nkọwa
Ngwa nje DNA/RNA nke nje (Magnetic Beads) bụ efere ejikere akpaaka emejuru nke ọma maka sistemu ịsa ọcha HT.Nucleic acid (DNA/RNA) sitere na mgbagwoju anya nwere bead magnet n'ime ihe nchekwa ahaziri ahazi.A na-ekewakwa mgbagwoju anya beads / nucleic acid na lysates site na iji magnet.A na-ewepụkwa DNA/RNA dị ọcha mgbe emeziri ọnọdụ nchekwa.Teknụzụ magnetik pụrụ iche na-enyere aka ịsacha acid nucleic dị elu nke enweghị protein, nucleases na adịghị ọcha ndị ọzọ.
Ngwa ngwa
| ọdịnaya | Nkeji | |
| Efere 1 | Buffer MVN | 500 μL / nke ọma |
| Efere 2 | Ihe nchekwa DW1P | 500 μL / nke ọma |
| Efere 3 | Ihe nchekwa MWP | 600 μL / nke ọma |
| FineMag Particles G | 5 μl / nke ọma | |
| Efere 4 | ddH na-efu RNase2O | 100 μL / nke ọma |
| 96 Ndụmọdụ Comb | 1 | |
Atụmatụ na uru:
- Dị mma: na-enweghị proteinase K, enwere ike ịpụta ya ozugbo site na ịgbakwunye 200 μl nke ihe atụ.
- Ngwa ngwa: kwalite mmemme ahụ, ịsachapụ otu nzọụkwụ, mmịpụta ahụ na-ewe naanị nkeji iri na abụọ iji mezue mmịpụta nke nlele 96.
- Ọdịmma: bulie ihe nchekwa na bead magnetik, na-eme ka mmetụta nke mmịpụta nje dịkwuo mma, ma belata ihe egwu nke "ihe ọjọọ"
- Ndakọrịta dị oke mma: ndakọrịta dị elu na ngwọta nchekwa dị elu na reagents nchọpụta ala, ezigbo mgbanwe na nkwụsi ike nke nsonaazụ mmịpụta.
Ngwa ngwa
Emebere ngwa nje DNA/RNA Kit (Magnetic Beads) maka ịsacha ngwa ngwa nke nucleic acid dị elu (RNA na DNA) site na nje na nlele dịka swabs, asọ, ọbara, mmiri ara, Plasma/Serum, mmamịrị na mgbasa ozi nje na-ebufe nje (VTM).
Nchekwa na Shelf ndụ
Enwere ike ịchekwa reagent niile na ụlọ okpomọkụ (15-25°C) maka ọnwa 12.
Dee ozi gị ebe a ziga anyị ya
