Blood SuperDirectᵀᴹ PCR Kit-Heparin Blood Direct PCR Master Mix for Genotyping of Blood
Nkọwa ngwa:
Jiri heparin anticoagulated ọbara niile ozugbo dị ka ndebiri maka nkwalite PCR, yana mmetụta dị elu yana arụmọrụ nkwalite dị elu.
◮Ọ dịghị mkpa maka ịdị ọcha DNA na-ewe oge ma dị oke ọnụ, enweghị usoro ọgwụgwọ, na iji ọbara dum mee ihe ozugbo dị ka ndebiri PCR.
◮Usoro ahụ nwere ikike mmụba siri ike ma nwee ike ịchọpụta genome na iberibe DNA lekwasịrị anya nke mba ọzọ n'ime ọbara.
◮2× Dị mfeTMMix-Heparin Nnukwu nnabata ọbara: ruo 45% n'ọbara mmadụ yana 20% n'ọbara òké.
◮A na-emechi ihe nlele ahụ nke ọma, yabụ na ọ dịghị mkpa ichegbu onwe gị maka mmetọ nlele na nsonaazụ PCR adịgboroja.
Nkọwapụta
200 × 20μl rxns, 2000 × 20μl rxns
2× Dị mfeTMMix-Heparin: Nwere D-Taq DNA Polymerase emezigharịrị pụrụ iche, dNTPs, MgCl2, ihe nchekwa mmeghachi omume, nkwalite mmeghachi omume PCR, optimizer, stabilizer, wdg. Na mmeghachi omume PCR, gbakwunye ọbara zuru oke nke anticoagulated kwesịrị ekwesị, primers, na ddH.2O na 2× SuperEasy kwekọrọTMMix-Heparin ga-eji na mmeghachi omume PCR.
6 × DNA Loading Buffer: Ihe nkpuchi anaghị enwe SDS.A na-atụ aro ka iji 6 × DNA Loading Buffer gụnyere na kit mgbe ị na-eme agarose gel electrophoresis iji nweta nsonaazụ electrophoresis dị mma.Ejila Loading Buffer nwere SDS, ma ọ bụghị ya, a ga-enwe nnukwu ọdụ ọkụ na-egbuke egbuke n'okporo ụzọ n'oge electrophoresis, nke ga-emetụta nsonaazụ nnwale.
Ngwa ngwa
| 2× SuperEasyTM Mix-Heparin |
| Ihe nchekwa 6 × DNA na-ebufe |
| Ntuziaka |
Atụmatụ & uru
-Ọ dịghị mkpa maka ịdị ọcha DNA na-ewe oge ma dị oke ọnụ, enweghị pretreatment, na iji ọbara dum mee ihe ozugbo dị ka ndebiri PCR.
- Usoro ahụ nwere ikike mmụba siri ike ma nwee ike ịchọpụta genome na iberibe DNA lekwasịrị anya nke mba ọzọ n'ime ọbara.
-2× SuperEasyTMMix-Heparin Nnukwu nnabata ọbara: ruo 45% n'ọbara mmadụ yana 20% n'ọbara òké.
- A na-emechi ihe nlele ahụ n'ụzọ zuru ezu, yabụ na ọ dịghị mkpa ichegbu onwe gị maka mmetọ nlele na nsonaazụ PCR adịgboroja.
Ngwa ngwa
A raara onwe ya nye maka iduzi PCR njirimara Heparin na-egbochi coagulated ọbara dum.(Gụnyere: ọbara mmadụ, ọbara òké, ọbara ọkụkọ, ọbara nnụnụ, ọbara ehi, ọbara nkịta, wdg)
Usoro ọrụ
Nchekwa na Shelf ndụ
Ekwesịrị ịchekwa ngwa a na -20 ℃.
2× Dị mfeTMMix-Heparin, Ọ bụrụ na ejiri ya ugboro ugboro, enwere ike ịchekwa ya na 4 Celsius maka nchekwa oge dị mkpirikpi (jiri n'ime ụbọchị 10).
6 × DNA Loading Buffer, Enwere ike ịchekwa ya na 4°C ma ọ bụ -20°C ogologo oge.
