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Ngwọta isi anọ maka njikwa mmetọ ngwaahịa PCR
1: Dochie ihe nnwale nnwale na oge Tọọ (NTC) njikwa adịghị mma ma megharịa ya ọtụtụ oge.Ozugbo achọpụtara na enwere mmetọ ngwaahịa PCR n'ime ụlọ nyocha, dochie ihe nnwale niile n'oge.Dị ka: regharịa na dozie primers, re-sterilize n'ọnụ pipette, E...GỤKWUO -
Enzymes RT-PCR abụọ na-arụ ọrụ abụọ
Ntụgharị ntụgharị ọdịnala enweghị ike ịnabata oke okpomọkụ (okpomọkụ kacha mma maka ọrụ MMLV bụ 37-50°C, yana AMV bụ 42-60°C).Enweghị ike ịtụgharị RNA viral dị mgbagwoju anya nke ọma ka ọ bụrụ cDNA n'obere okpomọkụ, na-ebute nrụpụta nrụpụta Mbelata ahụ.Tra...GỤKWUO -
Nucleic acid isothermal amplification technology
PCR bụ teknụzụ nkwalite nucleic acid kachasị eji eme ihe ma ejiri ya mee ihe n'ihi nghọta ya na nkọwapụta ya.Agbanyeghị, PCR chọrọ denaturation thermal ugboro ugboro na enweghị ike iwepu oke nke ịdabere na ngwa na akụrụngwa, nke na-egbochi ngwa ya na ụlọ ọgwụ…GỤKWUO -
Kedu ihe bụ ọgwụ mgbochi mRNA?
Kedu ihe bụ ọgwụ mgbochi mRNA Ogwu mRNA na-ebufe RNA na sel nke ahụ iji kwupụta ma mepụta antigens protein mgbe mgbanwe dị mkpa na vitro, si otú ahụ na-eduga anụ ahụ iji mepụta nzaghachi mgbochi megide antigen, si otú ahụ gbasaa bod ...GỤKWUO -
PCR teknụzụ
PCR (mmeghachi omume yinye polymerase) bụ otu n'ime teknụzụ mmụba DNA in-vitro, nwere akụkọ ihe karịrị afọ 30.Kary Mullis nke Cetus, USA sụrụ ụzọ teknụzụ PCR na 1983. Mullis tinyere akwụkwọ maka patent PCR na 1985 wee bipụta akwụkwọ agụmakwụkwọ PCR mbụ na Sayensị n'otu ...GỤKWUO -
Nchọpụta coronavirus ọhụrụ, site na nucleic acid, antibody ruo antigen, otu esi ahọrọ nhazi nke ọrịa ahụ?
Ọrịa na-efe efe agbanweela ụwa.N'ụwa niile, gọọmentị mba niile na-eche nnukwu ihe ịma aka ihu na mgbochi na njikwa ọrịa.N'oge ọrịa COVID-19, China nọ n'akụkụ anọ nke mgbochi na nzaghachi nzaghachi (mgbochi, nchọpụta, njikwa na igodo iji su ...GỤKWUO -
Ụzọ anọ maka igbochi mmetọ ngwaahịa PCR
Ụlọ SOP Sistem Mepụta PCR nnwale SOP iji hazie omume nke ndị ọrụ nnwale.Ndị na-eme nnwale ahụ na-agbasosi usoro ọrụ ahụ anya, ma belata mmetọ PCR nke ihe mmadụ nwere ike ịkpata ma ọ bụ gbochie mmetọ mmetọ n'ime ọrụ ahụ.Na mgbakwunye...GỤKWUO -
Otu esi emeziwanye arụmọrụ nke ntụgharị ntụgharị LncRNA?
Ndu ogologo RNA na-enweghị koodu, lncRNA bụ RNA na-abụghị koodu nke nwere ogologo karịa 200 nucleotides, n'ozuzu n'etiti 200-100000 nt.lncRNA na-ahazi okwu mkpụrụ ndụ ihe nketa na epigenetic, transcript na post-transcriptional ọkwa, na-ekere òkè na X chromosome silencing, genome imprinting na chroma...GỤKWUO -
Enwere ike ibufe ya site na mmadụ gaa na onye n'ime sekọnd iri na anọ.Kedu ihe mere ụdị Delta "na-efe efe na-efe efe" na Zhong Nanshan Pass?
Isi mmalite ntụgharị: Onye nchịkọta akụkọ otu WuXi AppTec Na Guangzhou, China, ndị uwe ojii na-ahụ maka enyemaka nyocha ọrịa weputara vidiyo onyunyo: N'otu ụlọ oriri na ọ restaurantụ ,ụ ahụ, ha abụọ na-abanye n'ime ụlọ ịsa ahụ n'otu n'otu na-enweghị mmetụ anụ ahụ.Naanị 14 sekọnd nke ngalaba-e...GỤKWUO -
Nchọpụta mkpụrụ ndụ SNP na nchọpụta
Mgbe onye oka mmụta America bụ Eric S. Lander kwadoro otu nucleotide polymorphism (SNP) dị ka akara mkpụrụ ndụ ọgbọ nke atọ na 1996, SNP ejirila ya mee ihe n'ọtụtụ ebe na nyocha njikọ akụ na ụba, nhazi map njikọ mkpụrụ ndụ ihe nketa, na nyocha mkpụrụ ndụ ihe nketa mmadụ., D...GỤKWUO -
Sayensị na-ewu ewu |Otu esi achọpụta coronavirus ọhụrụ site na qPCR
COVID-19 bụ ọrịa na-efe efe nke ọrịa siri ike iku ume Coronavirus Ụdị 2 na-ebute. Mgbe mmadụ butere ọrịa, ihe mgbaàmà ndị a na-ahụkarị gụnyere ahụ ọkụ, ụkwara, na mkpụmkpụ ume.Enwere ike ịnakọta ihe nlele ndị a na-eji nyocha site na swabs nasopharyngeal ma ọ bụ swabs oropharyngeal.Kedu ihe m...GỤKWUO -
Ị ka na-eji teknụzụ emepụtara afọ 30 gara aga wepụ RNA?
Ị ka na-eji Trizol wepụ RNA?Gosh, ekwela ka m chegbuo maka ahụike gị!Trizol na-achọ ịbụ onye a ga-ekewapụ, agwala m na ị ka nọ n'ọchịchịrị?Akụkụ bụ isi nke Trizol reagent bụ phenol, yana mgbakwunye organic reagents dị ka chloroform na nnwale ahụ.GỤKWUO
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