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Kit ForeDirect RT-qPCR
◮Maka nkwalite RNA ozugbo sitere na mkpokọta swab na-enweghị usoro nchacha RNA.Ngwa a na-emecha okirikiri qRT-PCR n'ime otu elekere.Ngwakọta enzyme bụ ngwakọta kachasị nke Reverse Transcriptase, Hot-Start Taq DNA polymerase, RNase inhibitor.Ihe nchekwa mmeghachi omume nwere ihe niile achọrọ, gụnyere akụrụngwa nchekwa kachasị, Mg2+, dUTP, na dNTPs.
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Kit DNA Mwepu FFPE DNA si na Sample FFPE
Mee ngwa ngwa DNA genomic dị elu site na anụ ahụ ndị etinyere na paraffin dị ka akụkụ paraffin na paraffin blocks.
◮Enweghị mmetọ RNase:Ogidi DNA-naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepụ RNA na DNA genomic na-agbakwunyeghị RNase n'oge nnwale ahụ, na-egbochi ụlọ nyocha ahụ site na RNase exogenous metọọ.
◮Ọsọ ọsọ:Foregene Protease nwere ọrụ dị elu karịa proteases yiri ya, ma na-agbari ihe nlele anụ ahụ ngwa ngwa;arụ ọrụ ahụ dị mfe, yana ọrụ mmịpụta DNA nke genomic nwere ike mechaa n'ime nkeji 20-80.
◮Dị mma:A na-eme centrifugation na okpomọkụ nke ụlọ, ọ dịghịkwa mkpa maka 4 Celsius C obere okpomọkụ centrifugation ma ọ bụ ethanol precipitation nke DNA.
◮Nchekwa:Ọ dịghị organic reagent mmịpụta a chọrọ.
◮Ịdị mma Dị elu:DNA genomic nke ewepụtara nwere nnukwu iberibe, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe nyocha dị iche iche chọrọ.
◮Sistemụ Micro-elution:Ọ nwere ike ịbawanye ntinye nke DNA genomic, nke dị mma maka nchọpụta ala ma ọ bụ nnwale.
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Ngwa PCR dị ọcha PCR Sistemụ nhichapụ maka DNA dị ọcha
Mee ka ọ dị ọcha ngwa ngwa ma nweta iberibe DNA dị elu site na usoro PCR;Ọ bụrụ na ịchọrọ ikewapụ wee nweta iberibe DNA akọwapụtara, biko họrọ ngwa mgbake jel.
◮ Ọtụtụ mgbake DNA: Enwere ike ị nwetaghachi iberibe DNA dị mkpụmkpụ dịka 60bp ma buru ibu dịka 10kb.
◮ Nnukwu mgbake arụmọrụ: The mgbake arụmọrụ bụ n'ozuzu karịa 80%, na kasị elu nwere ike iru karịa 95%.
◮ Ọsọ ọsọ:Ọ dị mfe iji rụọ ọrụ, enwere ike ịmechaa mgbake DNA n'ime nkeji iri na ise ma wepụ primer dimer ozugbo na-enweghị mgbake gel.
◮ Safọ: Ọ dịghị organic reagent mmịpụta a chọrọ.
◮ Ogo dị elu: Iberibe DNA ndị ahụ enwetara dị oke ọcha, nke nwere ike izute nnwale dị iche iche na-esote.
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Ihe nnọchi anya mwepụta nlele
◮ Ọ dị mfe ịrụ ọrụ:sample lysis mgbe sample collection
Ngwa ngwa na adaba:Ọ dịghị mkpa nucleic acid mmịpụta, kwesịrị ekwesị maka nchọpụta nnukwu ọnụ ọgụgụ
◮ Oke ngwa ngwa:PCR ozugbo nchịkọta sample
◮ Nchekwa na ntụkwasị obi:Ngwa ngwa emenyụ nje virus, kwe nkwa nchekwa ruo oke kachasị
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RT-PCR Easyᵀᴹ I (Otu Nzọụkwụ)
◮Ngwa otu nzọụkwụ na-enyere aka ịtụgharị ntụgharị na PCR ka eme ya n'otu tube ahụ.Naanị ọ ga-achọ ịgbakwunye RNA template, kpọmkwem PCR primers na RNase-Free ddH2O.
◮Enwere ike ime nyocha ọnụọgụ RNA ozugbo na ngwa ngwa.
◮Ngwa ahụ na-eji ihe ntụgharị ntụgharị pụrụ iche nke Foregene na Foregene HotStar Taq DNA Polymerase jikọtara ya na sistemu mmeghachi omume pụrụ iche iji melite arụmọrụ nkwalite na nkọwapụta mmeghachi omume ahụ nke ọma.
◮Usoro mmeghachi omume kachasị mma na-eme ka mmeghachi omume ahụ nwee mmetụta nchọpụta dị elu, nkwụsi ike okpomọkụ siri ike, na ndidi ka mma.
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RT-PCR Easyᵀᴹ II (nzọụkwụ abụọ)
◮Enwere ike ime nyocha ọnụọgụ RNA ozugbo na ngwa ngwa.
◮Ngwa ahụ na-eji ihe ntụgharị ntụgharị pụrụ iche nke Foregene na Foregene HotStar Taq DNA Polymerase jikọtara ya na sistemu mmeghachi omume pụrụ iche iji melite arụmọrụ nkwalite na nkọwapụta mmeghachi omume ahụ nke ọma.
◮ Usoro mmeghachi omume kachasị mma na-eme ka mmeghachi omume ahụ nwee mmetụta nchọpụta dị elu, nkwụsi ike okpomọkụ siri ike, na ndidi ka mma.
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Oke Obere DNA Obere Kit Genomic DNA Extraction Kits from Mouse Tail
Ngwa kachasị ngwa n'ụwa maka ịwepụta DNA ọdụ ọdụ òké, nke nwere ike wepụ DNA genomic dị elu site na ọdụ òké n'ime 1 hour.
◮Enweghị mmetọ RNase:Ogidi DNA-naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepụ RNA na DNA genomic na-agbakwunyeghị RNase n'oge nnwale ahụ, na-egbochi ụlọ nyocha ahụ site na RNase exogenous metọọ.
◮Ọsọ ọsọ:Foregene Protease nwere ọrụ dị elu karịa proteases yiri ya, ma na-agbari ihe nlele anụ ahụ ngwa ngwa;arụ ọrụ ahụ dị mfe, yana ọrụ mmịpụta DNA nke genomic nwere ike mechaa n'ime nkeji 20-80.
◮Dị mma:A na-eme centrifugation na okpomọkụ nke ụlọ, ọ dịghịkwa mkpa maka 4 Celsius C obere okpomọkụ centrifugation ma ọ bụ ethanol precipitation nke DNA.
◮Nchekwa:Ọ dịghị organic reagent mmịpụta a chọrọ.
◮Ịdị mma Dị elu:DNA genomic nke ewepụtara nwere nnukwu iberibe, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe nyocha dị iche iche chọrọ.
◮Sistemụ Micro-elution:Ọ nwere ike ịbawanye ntinye nke DNA genomic, nke dị mma maka nchọpụta ala ma ọ bụ nnwale.
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Ngwa Ngwa Mwepu DNA Bacterial Bacterial Genomic DNA Extraction Ngwa Ngwa dị ọcha
Mee ngwa ngwa DNA genomic dị elu site na nje bacteria na oge uto logarithmic.
◮Enweghị mmetọ RNase:Ogidi DNA-naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepụ RNA na DNA genomic na-agbakwunyeghị RNase n'oge nnwale ahụ, na-egbochi ụlọ nyocha ahụ site na RNase exogenous metọọ.
◮Ọsọ ọsọ:Foregene Protease nwere ọrụ dị elu karịa proteases yiri ya, ma na-agbari ihe nlele anụ ahụ ngwa ngwa;arụ ọrụ ahụ dị mfe, yana ọrụ mmịpụta DNA nke genomic nwere ike mechaa n'ime nkeji 20-80.
◮Dị mma:A na-eme centrifugation na okpomọkụ nke ụlọ, ọ dịghịkwa mkpa maka 4 Celsius C obere okpomọkụ centrifugation ma ọ bụ ethanol precipitation nke DNA.
◮Nchekwa:Ọ dịghị organic reagent mmịpụta a chọrọ.
◮Ịdị mma Dị elu:DNA genomic nke ewepụtara nwere nnukwu iberibe, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe nyocha dị iche iche chọrọ.
◮Sistemụ Micro-elution:Ọ nwere ike ịbawanye ntinye nke DNA genomic, nke dị mma maka nchọpụta ala ma ọ bụ nnwale.
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CCND1/IGH Dual Dual Fusion Probe
Dị ka ụkpụrụ nke DNA base complementary pairing, CCND1 oroma-acha ọbara ọbara nyocha na IGH nyocha akwụkwọ ndụ akwụkwọ ndụ ka ejiri mee ka usoro DNA lekwasịrị anya n'ime oghere ahụ wee hụ ma nyochaa ozi steeti dị na oghere n'okpuru microscope fluorescence.
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Nyocha agba abụọ RB1/1q21
Fluorescence in situ hybridization (FISH) dabere n'ụkpụrụ nke njikọta njikọta nke ntọala DNA, yana nhụta nke akara hybridization nke nyocha DNA nke akpọrọ fluorescence na usoro ebumnuche DNA ya na oghere cell n'okpuru microscope fluorescence.
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HER2/CSP17 Dual Agba nyocha
Fluorescence in situ hybridization (FISH) dabere n'ụkpụrụ nke njikọta njikọta nke ntọala DNA, yana nhụta nke akara hybridization nke nyocha DNA nke akpọrọ fluorescence na usoro ebumnuche DNA ya na oghere cell n'okpuru microscope fluorescence.
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ETV6 Dual Agba Break Apart Probe
Dabere na ụkpụrụ njikọta njikọta nke DNA base, e jiri nyocha ETV6 oroma-acha ọbara ọbara na nyocha akwụkwọ ndụ akwụkwọ ndụ ETV6 mee ka usoro DNA lekwasịrị anya n'ime oghere ahụ, na-ahụ ma nyochaa ozi steeti mkpụrụ ndụ dị na oghere n'okpuru microscope fluorescence.
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