Ngwa nkewapụta DNA nke osisi Genomic Plant DNA Cleaning Kits Protocol Reagents
Nkọwapụta
Nkwadebe 50, nkwadebe 100, nkwadebe 250
Ngwa a na-eji kọlụm DNA naanị nwere ike jikọta DNA, Foregene protease na sistemu nchekwa pụrụ iche, nke na-eme ka nchacha nke DNA mkpụrụ ndụ ihe ọkụkụ dị mfe.Enwere ike nweta DNA genomic dị elu n'ime nkeji 30, nke na-ezere mmebi nke DNA genomic.
Akpụkpọ anụ silica gel naanị DNA nke ejiri na kọlụm ogho bụ ihe ọhụrụ pụrụ iche nke Foregene, nke nwere ike jikọta DNA nke ọma na nke ọma, wee bulie mwepụ nke RNA, protein adịghị ọcha, ion, polysaccharides, polyphenols na ogige organic ndị ọzọ.
Ngwa ngwaahịa
| Buffer PL1, ihe nchekwa PL2 |
| Buffer PW, Buffer WB, Buffer EB |
| Protease nke mbụ |
| DNA-naanị kọlụm |
| Ntuziaka |
Atụmatụ & uru
∎ Enweghị mmetọ RNase: Ogidi DNA naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepụ RNA na DNA genomic na-enweghị RNase agbakwunyere n'oge nnwale ahụ, na-egbochi ụlọ nyocha ahụ site na RNase dịpụrụ adịpụ metọọ ụlọ nyocha.
■ Ọsọ ọsọ: Foregene Protease nwere ọrụ dị elu karịa ụdị proteases ma na-agbari ihe nlele anụ ahụ ngwa ngwa.
∎ Dị Mfe: Enwere ike ịrụcha ọrụ mmịpụta DNA n'ime nkeji iri atọ.
■ Dị Mma: A na-eme centrifugation na ụlọ okpomọkụ, ọ dịghị 4 ℃ obere okpomọkụ centrifugation ma ọ bụ ethanol precipitation nke DNA chọrọ.
■ Nchekwa: ọ dịghị organic reagenti a chọrọ.
■ Ọdịmma dị elu: DNA genomic a sachara nwere nnukwu iberibe, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nwere ike izute ihe nyocha dị iche iche chọrọ.
Ngwa ngwa
Kwesịrị ekwesị maka mmịpụta na sachaa DNA genomic site na anụ ahụ osisi ọhụrụ ma ọ bụ oyi kpọnwụrụ.
Usoro ọrụ
Eserese
Nchekwa na Shelf ndụ
Enwere ike ịchekwa ngwa ahụ maka ọnwa 12 na okpomọkụ nke ụlọ (15-25 ℃) na 2-8 ℃ maka ogologo oge.
Ihe ngwọta Foregene Protease Plus nwere usoro pụrụ iche, nke na-arụ ọrụ mgbe echekwara ya na ụlọ okpomọkụ ruo ogologo oge (ọnwa 3);ọrụ ya na nkwụsi ike ga-aka mma mgbe echekwara ya4 ℃, n'ihi ya, a na-atụ aro ka ịchekwa ya na 4 ℃, cheta na ị gaghị echekwa na -20 ℃.
Ntuziaka nyocha nsogbu
The following is an analysis of the problems that may be encountered in the extraction of plant genomic DNA, hoping to be helpful to your experiments. In addition, for other experimental or technical problems other than operation instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us: 028-83360257 or E-mali: Tech@foregene.com.
Mkpụrụ dị ala ma ọ bụ enweghị DNA
A na-enwekarị ọtụtụ ihe na-emetụta mkpụrụ nke DNA genomic, gụnyere isi iyi nke sample, afọ nke sample, ọnọdụ nchekwa nke sample, na ọrụ.
Enweghị ike ịnweta DNA genomic n'oge mmịpụta
1. A na-echekwa ihe nlele anụ ahụ na-ekwesịghị ekwesị ma ọ bụ chekwaa ya ogologo oge, na-ebute mmebi nke DNA genomic.
Nkwanye: Chekwaa nlele anụ ahụ na nitrogen mmiri mmiri ma ọ bụ -20°C;nwaa iji ihe nlele anakọtara ọhụrụ maka mmịpụta DNA genomic.
2. Obere ihe nlele nwere ike ime ka a ghara iwepụta DNA genomic kwekọrọ.
Aro: Maka ihe nlele anụ ahụ nke echekwara ogologo oge ma ọ bụ nwere mmebi DNA nke genomic siri ike, enwere ike ịbawanye ọnụọgụ anụ ahụ nke ọma iji wepụta DNA mkpụrụ ndụ ihe nketa.Enwere ike ikpebi ọnụọgụ nke nlele ahụ dịka mkpa DNA si dị, mana ihe nlele ọhụrụ ekwesịghị ịfe 100mg, ihe nlele akọrọ ekwesịghị ịfe 30mg.
3. A naghị etinye ihe nlele ahụ na mmiri mmiri nitrogen ma ọ bụ tinye ya ogologo oge mgbe mmiri mmiri gachara.
Aro: N'oge mmịpụta DNA, ihe nlele ahụ kwesịrị ka ejiri nitrogen mmiri mee ala nke ọma iji mebie mgbidi cell;mgbe ị na-egweri, biko nyefee ntụ ntụ ntụ ntụ na PL1 preheated na 65°C ozugbo enwere ike (ozugbo ntụ ntụ ala gbazere, DNA genomic ga-amalite ịda ngwa ngwa).
4. Nchekwa na-ezighi ezi nke Foregene Protease na-ebute ọrụ belata ma ọ bụ adịghị arụ ọrụ.
Nkwanye: Kwenye ọnọdụ nchekwa nke Foregene Protease ma ọ bụ dochie ya na Foregene Protease ọhụrụ maka enzymatic hydrolysis.
5. A na-echekwa ngwa ahụ n'ụzọ na-ekwesịghị ekwesị ma ọ bụ chekwaa ya ogologo oge, na-eme ka ụfọdụ ihe dị na ngwa ahụ daa.
Nkwanye: Zụrụ ngwa ihe mmịpụta DNA genomic osisi ọhụrụ maka ọrụ ndị metụtara ya.
6. Iji ngwa na-ekwesịghị ekwesị.
Aro: Zụrụ ngwa nkewapụ DNA osisi nke ewepụtara maka nlele maka mmịpụta na sachaa DNA genomic osisi.
7. Buffer WB na-agbakwunyeghị ammiri ara ehi ethanol.
Nkwanye: Gbaa mbọ hụ na ịtinye oke ethanol zuru oke na Buffer WB.
8. Awụpụghị eluent ahụ n'ime akpụkpọ ahụ silica nke ọma.
Aro: Tinye eluent ahụ ebugoro ọkụ na 65℃ịdaba n'etiti akpụkpọ anụ silica gel, ma hapụ ya na ụlọ okpomọkụ maka nkeji 5 iji mee ka arụmọrụ elution dịkwuo elu.
Mwepụta iji nweta DNA genomic dị ala
1. A na-echekwa ihe nlele ahụ na-ekwesịghị ekwesị ma ọ bụ chekwaa ya ogologo oge, na-ebute mmebi nke DNA genomic.
Nkwanye: Chekwaa nlele anụ ahụ na -20℃;gbalịa iji ihe nlele anụ ahụ anakọtara ọhụrụ maka mmịpụta DNA genomic.
2. Ọ bụrụ na ọnụọgụ anụ ahụ dị ntakịrị, DNA genomic amịpụtara ga-adị ntakịrị.
Aro: Ụfọdụ ihe atụ osisi nwere mmiri, dị ka ihe ọkụkụ dị n'ime mmiri dị ka algae, wdg, enwere ike ịbawanye dose ahụ nke ọma ma ọ bụ mee ka mmiri kpoo ntakịrị tupu ọrụ ahụ.
3. A naghị ejikọta ihe atụ ndị ahụ nke ọma na mmiri mmiri nitrogen ma ọ bụ hapụ ya na ụlọ okpomọkụ ruo ogologo oge mgbe egwerichara.
Aro: Mmiri nitrogen egweri ga-ezuru, na sample cell mgbidi kwesịrị ka agbajikwa dị ka o kwere mee;ozugbo egwerichara, a ga-ebufe ntụ ntụ ntụ na 65℃Buffer PL1 preheated maka nzọụkwụ ọzọ.
4. Anaghị eji ngwa ziri ezi.
Nkwanye: Jiri ngwa nkewapụta DNA nke osisi raara onwe ya nye wepụta na sachaa DNA genomic osisi.
5. Nchekwa na-ezighi ezi nke Foregene Protease na-ebute ọrụ belata ma ọ bụ adịghị arụ ọrụ.
Nkwanye: Kwenye ọnọdụ nchekwa nke Foregene Protease ma ọ bụ dochie ya na Foregene Protease ọhụrụ maka enzymatic hydrolysis.
6. Eluent nsogbu
Nkwanye: Biko jiri Buffer EB maka elution;Ọ bụrụ na ị na-eji ddH2O ma ọ bụ eluent ndị ọzọ, hụ na pH nke eluent dị n'etiti 7.0-8.5.
7. Anaghị agbapụta eluent nke ọma
Aro: Biko tinye dobe elution n'etiti akpụkpọ anụ silica ma hapụ ya na ụlọ okpomọkụ maka nkeji 5 iji mee ka ọrụ elution dịkwuo elu.
8. Olu eluent dị obere
Aro: Biko jiri eluent maka genomic DNA elution dị ka ntuziaka si dị, opekata mpe, ọ bụghị ihe na-erughị 100μl.
DNA genomic ewepụtara na ịdị ọcha dị ala
Ịdị ọcha dị ala nke DNA genomic ga-eduga na ọdịda ma ọ bụ mmetụta na-adịghị mma nke nnwale ndị dị n'okpuru ala, dị ka: enweghị ike igbutu enzyme ahụ, na PCR enweghị ike ịnweta mpempe mkpụrụ ndụ e lekwasịrị anya.
1. Mmetọ protein dị iche iche, mmetọ RNA.
Nyocha: A naghị eji Buffer PW sachaa kọlụm;Ejighị Buffer PW sachaa kọlụm na ọsọ centrifugation ziri ezi.
Aro: gbalịa hụ na ọ dịghị mmiri ozuzo na elu mgbe a na-agafe na kọlụm;jide n'aka na ị ga-eji Buffer PW sachaa kọlụm nchacha dịka ntuziaka ahụ siri dị, na enweghị ike ịhapụ usoro a.
2. Mmetọ ion adịghị ọcha.
Nyocha: E wepụrụ kọlụm ịsa ahụ Buffer WB ma ọ bụ saa ya naanị otu ugboro, butere mmetọ ionic fọdụrụnụ.
Nkwanye: Gbaa mbọ hụ na ị na-eji Buffer WB sachaa ugboro abụọ dịka ntuziaka siri dị ka iwepu ion fọdụrụ ka o kwere mee.
3. RNase ofufe.
Nyocha: Exogenous RNase na-agbakwunye na nchekwa;Arụ ọrụ ịsa ahụ na-ezighi ezi na Buffer PW ga-ebute RNase fọdụrụnụ ma metụta ọrụ nnwale RNA mgbada, dị ka ntụgharị in vitro.
Aro: Foregene usoro nucleic acid mmịpụta ngwa nwere ike wepu RNA na-enweghị mgbakwunye RNase, na ihe niile reagents na Plant DNA Isolation Kit adịghị mkpa RNase;jide n'aka na ị ga-eji Buffer PW sachaa kọlụm nchacha dịka ntuziaka ahụ siri dị, na enweghị ike ịhapụ usoro a.
4. Ethanol fọdụrụnụ.
Nyocha: Mgbe ejiri Buffer WB sachaa kọlụm nchacha ahụ, ọ nweghị centrifugation tube efu emere.
Nkwanye: Soro ntuziaka maka centrifugation tube efu kwesịrị ekwesị.
Akwụkwọ ntuziaka:
Akwụkwọ ntuziaka ngwa mwepu DNA nke osisi
