Sistemụ njirimara DNA foregene 20A (Nwepụta DNA efu)
Nkọwa ngwa:
Sistemụ njirimara DNA nke Foregene 20A na-eji teknụzụ akara fluorescent nwere agba ise na-amụba n'otu oge 19 STR loci na 1 loci mmekọahụ n'ime otu tube.Mgbe ị na-ekpuchi 13 CODIS core loci, 20 core loci na-emezu ihe achọrọ nke Ministry of Public Security for autosomal STR kit.Ọ bụ onye aka nri nke iwu nchekwa data DNA nke nyocha na njirimara ndị ikwu ikpe.
Nkọwa
Sistemụ njirimara DNA nke Foregene 20A na-eji teknụzụ akara fluorescent nwere agba ise na-amụba n'otu oge 19 STR loci na 1 loci mmekọahụ n'ime otu tube.Mgbe ị na-ekpuchi 13 CODIS core loci, 20 core loci na-emezu ihe achọrọ nke Ministry of Public Security for autosomal STR kit.Ọ bụ onye aka nri nke iwu nchekwa data DNA nke nyocha na njirimara ndị ikwu ikpe.
Ọdịnaya ngwa
| Ngwa Componet | Aha Componet | Olu (μl / tube) | Ọnụọgụ (tube) |
| PCR preaction reagent | Ihe nchekwa mmeghachi omume 2.5 × PCR Ⅲ | 1000 | 2 |
| 5×20A primer mix | 500 | 2 | |
| DNA njikwa 9947A (1ng/μl) | 25 | 1 | |
| Mmiri a tọhapụrụ | 1700 | 2 | |
| Polymerase | Taq polymerase Ⅲ | 40 | 2 |
| PCR Reaction reagent | Allelic ladder20A | 40 | 1 |
| Ọkpụrụkpụ Molecular dị n'ime ọkọlọtọORG 500 | 150 | 2 | |
| Rịba ama | |||
Nyocha data
1. Mepee Genemapper ID software.Mgbe ị na-eji ngwa a na nke mbụ, ịkwesịrị ibubata ogwe, usoro nyocha, mmadụ iri abụọ na ise, mmadụ iri abụọ na ise, mmadụ iri atọ na ise, mmadụ iri atọ na ise
2. Bubata data electrophoresis, họrọ usoro nyocha kwekọrọ na ya dị ka panel, usoro nyocha, na ọkọlọtọ nha, ma gbanwee ụdị nlele nke Ladder na "Allelic Ladder" na kọlụm "Sample Type";malite nyocha data.
Nchekwa Reagent
1. Mgbe ị nwetasịrị ngwa ahụ oyi kpọnwụrụ na ice akọrọ ma ọ bụ ngwugwu ice plastik, ọ bụrụ na ejighị ya nwa oge, biko chekwaa ya n'okpuru -20 Celsius ruo ogologo oge.
2. Mgbe ewepụrụ ngwa ahụ ma jiri ya mee ihe, a ga-echekwa ngwa ahụ na 4 Celsius C tupu mmeghachi omume ahụ iji zere oyi na-agbaze ugboro ugboro;Ekwesịrị ịchekwa enzyme Taq na -20 Celsius.
3. Mgbe mmeghachi omume ahụ gasịrị, a ga-edobe ngwa ahụ (gụnyere ụkpụrụ dị n'ime molekụla dị n'ime na ubube) na "ime ụlọ nchọpụta electrophoresis" na 4 Celsius C, zere oyi na-ekpo ọkụ ugboro ugboro na thawing, ma zere kọntaktị na ngwa mmeghachi omume tupu ime ihe iji zere mmetọ.
