Sistemụ njirimara DNA nke mbụ 27Y
Nkọwa ngwa:
Mbido Sistemụ njirimara DNA 27Y na-eji teknụzụ akara fluorescent nwere agba isii iji gbasaa 27 Y-STR loci n'otu oge.Gụnyere: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS448, DYS533, DYFY387 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
Ngwa ahụ nwere ikike mgbochi mgbochi dị mma, ma nwee ike ịbawanye akwụkwọ nzacha ozugbo, kaadị FTA, swab owu, kaadị saliva, swab ọnụ, yana enwere ike iji wepụ ma sachaa ndebiri DNA site na ihe ikpe.
Nkọwa
Mbido Sistemụ njirimara DNA 27Y na-eji teknụzụ akara fluorescent nwere agba isii iji gbasaa 27 Y-STR loci n'otu oge.Gụnyere: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS448, DYS533, DYFY387 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
Ngwa ahụ nwere ikike mgbochi mgbochi dị mma, ma nwee ike ịbawanye akwụkwọ nzacha ozugbo, kaadị FTA, swab owu, kaadị saliva, swab ọnụ, yana enwere ike iji wepụ ma sachaa ndebiri DNA site na ihe ikpe.
Ọdịnaya ngwa
| Ngwa nyocha reagent | Aha akụrụngwa | Olu (μl/tube) | Ọnụọgụ (tube) |
| Ngwa premeaction PCR | 2 × PCR Reaction Master Mix | 1250 | 2 |
| Ngwakọta mbụ nke 5 x 27Y | 500 | 2 | |
| Ọkpụrụkpụ DNA 9948 | 20 | 2 | |
| Mmiri a tọhapụrụ | 1700 | 2 | |
| Ngwa mmeghachi omume mgbe PCR | 27Y allele ngwakọta Ubube | 20 | 2 |
| Ọkọlọtọ molikula oroma dị n'ime ORG500 | 150 | 2 |
Nyocha data
1. Mepee ngwa ngwa Gene Mapper ID.Mgbe ị na-eji ngwa a na nke mbụ, ịkwesịrị ibubata ogwe, usoro nyocha, mmadụ iri abụọ na ise, mmadụ iri abụọ na ise, mmadụ iri abụọ na ise, 450, 490, 490, 490, 490, 490, 400.
2. Bubata data electrophoresis, họrọ usoro nyocha kwekọrọ na ya dị ka panel, usoro nyocha, na ọkọlọtọ nha, ma gbanwee ụdị nlele nke Ladder na "Allelic Ladder" na kọlụm "Sample Type";malite nyocha data.
Nchekwa Reagent
1. Biko chekwaa ha n'okpuru -20 Celsius (ma ọ bụrụ na ejighị ya nwa oge), mgbe ị nwetasịrị ngwa ndị a jụrụ oyi na ice akọrọ ma ọ bụ gel ice ngwugwu.
2. Biko chekwaa ngwa ahụ na 4 Celsius C tupu mmeghachi omume ahụ ka ịzenarị oyi na agbaze ugboro ugboro, mgbe ewepụrụ ngwa ahụ maka ojiji,;Ekwesịrị ịchekwa enzyme ahụ na -20 Celsius.
2. Mgbe mmeghachi omume ahụ gasịrị, a ga-edobe ngwa ahụ (gụnyere ụkpụrụ dị n'ime molecular dị n'ime na ọkwa) na "ụlọ nchọpụta electrophoresis" na 4 Celsius C, zere oyi na-ekpo ọkụ ugboro ugboro na thawing, ma zere kọntaktị na ngwa mmeghachi omume tupu ime ihe iji zere mmetọ.
