Sistemụ njirimara DNA nke mbụ 30 AC (Ikpe)
Nkọwa ngwa:
Mbido Sistemụ njirimara DNA30A-C na-eji teknụzụ akara fluorescent nwere agba isiigbasaa 29autosomalSTR loci, 1 Ebe Amel, 1 Yindelebe, na1 saịtị mmekọ nwoke na nwanyị akọwapụtara, 2Saịtị njikwa mma dị n'ime, nke a na-eji na-amụba iheDNAtemplate ewepụtara ma sachaa yaCase material.
Nkọwa
Mbido Sistemụ njirimara DNA30A-C na-eji teknụzụ akara fluorescent nwere agba isiigbasaa 29autosomalSTR loci, 1 Ebe Amel, 1 Yindelebe, na1 saịtị mmekọ nwoke na nwanyị akọwapụtara, 2Saịtị njikwa mma dị n'ime, nke a na-eji na-amụba iheDNAtemplate ewepụtara ma sachaa yaCase material.
Ọdịnaya ngwa
Nkwadebe usoro nkwalite
Tebụl 2: Nkwadebe nke usoro mmịpụta na nkwalite ọkọlọtọ
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| Cndị na-akwado | 25ul sistem (µl) | Sistemu 10 μl (μl) |
| Ngwakọta Master | 2.5× PremixCⅡ | 10.0 | 4.0 |
| 5 x 30 Ngwakọta AC Primer | 5.0 | 2.0 | |
| DNA template | 2.5-10 | 1—4 | |
| Mmiri a tọhapụrụ | Mee ka olu mmeghachi omume dị 25μl | Mee ka olu mmeghachi omume10 μl |
Nyocha data
Eserese agbakwunyere 1:Map akara ubube Allelic
Eserese 2 agbakwunyere:Mpịnye DNA ọkọlọtọ 9948map
Nchekwa Reagent
1. Biko chekwaa n'okpuru -20 Celsius C,, mgbe ị natachara ngwa ndị a jụrụ oyi na ice akọrọ ma ọ bụ ngwugwu ice gel.
2. Biko chekwaa akụrụngwa pre-reaction 2.5 × premix CII na -20 Celsius mgbe ewepụrụ ngwa ahụ maka ojiji, a na-echekwa ihe ndị fọdụrụ na-emeghachi omume na 4 Celsius C iji zere ịfriza ugboro ugboro na thawing.Ọ bụrụ na otu usoro onunu ogwu dị obere, a na-atụ aro ka ịchekwa ya na -20 Celsius C mgbe ịchachara.
3. Chekwaa ihe ndị ahụ mgbe mmeghachi omume ahụ gasịrị na 4 Celsius C, zere ịza oyi na thawing ugboro ugboro, ma emetụla reagents tupu mmeghachi omume iji zere mmetọ.
