Sistemụ njirimara DNA nke mbụ 38Y-C (Ikpe)
Nkọwa ngwa:
Mbido Sistemụ njirimara DNA 38Y-C na-eji teknụzụ akara fluorescent nwere agba isii iji bulie 38 Y chromosome STR mpaghara, 3 Y-Ebe obibinaabụọ esịtidem mma akara loci n'otu ogemaka nkwalite Nlele ikpe ewepụtara na ndebiri DNA emere ka ọ dị ọcha.
Nkọwa
Mbido Sistemụ njirimara DNA 38Y-C na-eji teknụzụ akara fluorescent nwere agba isii iji bulie 38 Y chromosome STR mpaghara, 3 Y-Ebe obibinaabụọ esịtidem mma akara loci n'otu ogemaka nkwalite Nlele ikpe ewepụtara na ndebiri DNA emere ka ọ dị ọcha.
Ọdịnaya ngwa
Nkwadebe usoro nkwalite
Nkenkwadebe ngwakọtan of Ọkọlọtọmmịpụtana usoro nkwalite
| Ngwakọta Master | Cndị na-akwado | Sistemu 10 μl (μl) |
| 2.5 × PCR Master Mix C I | 4.0 | |
| 5 Ngwakọta 38Y-C Primer | 2.0 | |
| DNA template | 1- 4 | |
| Mmiri a tọhapụrụ | Jupụta ruo a mmeghachi omume olu10 μl |
Nyocha data
Eserese agbakwunyere 1:Map akara ubube Allelic
Eserese 2 agbakwunyere:Mpịnye DNA ọkọlọtọ 9948map
Nchekwa Reagent
1. Biko chekwaa ha n'okpuru -20 Celsius C (ma ọ bụrụ na ejighị ya nwa oge), mgbe ị nwetasịrị ngwa oyi na ice akọrọ ma ọ bụ gel ice ngwugwu.
2. Biko sotre pre-reaction akụrụngwa PCR master mix bụ na -20 Celsius C, mgbe a na-ewepụta ngwa maka ojiji na ndị fọdụrụ pre-reaction reagents na-echekwara na 4°C iji zere kefriza ugboro ugboro na thawing.Ọ bụrụ na otu usoro onunu ogwu dị obere, a na-atụ aro ka ịchekwa ya na -20 Celsius C mgbe ịchachara.
3. Chekwaa ihe ndị ahụ mgbe mmeghachi omume ahụ gasịrị na 4 Celsius C, zere ịza oyi na thawing ugboro ugboro, ma emetụla reagents tupu mmeghachi omume iji zere mmetọ.
