Sistemụ njirimara DNA nke mbụ Y Plus (anaghị ewepụta ya)
Nkọwa ngwa:
MbidoSistemụ njirimara DNA Y Plus na-eji teknụzụ akara fluorescent nwere agba isii iji gbasaa 20 Y chromosome core loci, loci 15 họọrọ, loci ọzọ 6 na 3 Y indel loci n'otu oge..
Nkọwa
MbidoSistemụ njirimara DNA Y Plus na-eji teknụzụ akara fluorescent nwere agba isii iji gbasaa 20 Y chromosome core loci, loci 15 họọrọ, loci ọzọ 6 na 3 Y indel loci n'otu oge..
Ọdịnaya ngwa
Nkwadebe usoro nkwalite
Nkenkwadebe mejupụtaranke standard(enweghị mmịpụta)usoro nkwalite
| Cndị na-akwado | 25 μlsistemu (μl) | Sistemu 10 μl (μl) | |
| Ngwakọta Master | 4 × Nna-ukwu MixVII | 6.25 | 2.5 |
| Ngwakọta 5×Y Plus Primer | 5.0 | 2.0 | |
| Mmiri a tọhapụrụ | 13.75 | 5.5 | |
| Blood unyi | Dayameta 1.2mm | Dayameta 1.2mm | |
| Tolu mmeghachi omume otal | 25 μl | 10 μl | |
Nyocha data
Eserese agbakwunyere 1:Map akara ubube Allelic
Eserese 2 agbakwunyere:Mpịnye DNA ọkọlọtọ 9948map
Nchekwa Reagent
1. Biko chekwaa n'okpuru -20 Celsius (Ọ bụrụ na ejighị ya nwa oge), mgbe ị nwetasịrị ngwa oyi na ice akọrọ ma ọ bụ gel ice ngwugwu.
2. Biko chekwaa akụrụngwa pre-reaction 4 × premix VII na -20 ° C, mgbe ewepụrụ ngwa ahụ maka ojiji, a na-echekwa ihe ndị ọzọ na-emeghachi omume na 4 Celsius C iji zere ịfriza ugboro ugboro na thawing.Ọ bụrụ na otu usoro onunu ogwu dị obere, a na-atụ aro ka ịchekwa ya na -20 Celsius C mgbe ịchachara.
3. Chekwaa ihe ndị ahụ mgbe mmeghachi omume ahụ gasịrị na 4 Celsius C, zere ịza oyi na thawing ugboro ugboro, ma emetụla reagents tupu mmeghachi omume iji zere mmetọ.
