Sistemụ njirimara DNA nke mbụ 25A-C (Ikpe)
Nkọwa ngwa:
Sistemụ njirimara DNA nke Foregene 25A-C na-eji teknụzụ akara ngosi fluorescence nwere agba isii na-amụba 23 autosomal STR loci, otu Amel loci, otu Yindel loci na ebe njikwa mma abụọ n'ime otu oge maka mmụba nke ndebiri DNA ewepụtara wee sachaa site na nlele ikpe.
Nkọwa
Sistemụ njirimara DNA nke Foregene 25A-C na-eji teknụzụ akara ngosi fluorescence nwere agba isii na-amụba 23 autosomal STR loci, otu Amel loci, otu Yindel loci na ebe njikwa mma abụọ n'ime otu oge maka mmụba nke ndebiri DNA ewepụtara wee sachaa site na nlele ikpe.
Ọdịnaya ngwa
Nkwadebe usoro nkwalite
Ngwakọta Nkwadebe nke Sistemụ mmịpụta na nkwalite
| Ngwa | Sistemu 10 μl (μl) | |
| Ngwakọta Master | 2.5 × PCR Premix C Ⅰ | 4.0 |
| Ngwakọta 10×25A-CPrimer | 1.0 | |
| DNA template | 1—5 | |
| Mmiri a tọhapụrụ | Mee ka olu mmeghachi omume nke 10μl |
Nyocha data
Eserese agbakwunyere 1:Gọọmenti genotyping nke allele
Eserese 2 agbakwunyere:Mpịnye DNA ọkọlọtọ 9948map
Nchekwa Reagent
1. Mgbe ị nwetasịrị ngwa ahụ, biko chekwaa ya n'okpuru -20 ℃ ma ọ bụrụ na ị naghị eji ya maka oge a.
2. Mgbe kit na-wepụrụ na-eji, na tupu mmeghachi omume akụrụngwa PCR premixed ngwọta na-echekwara na -20 ℃, na ndị fọdụrụ tupu mmeghachi omume reagents na-echekwara na 4 ℃ zere ugboro ugboro ifriizi-thaw.Ọ bụrụ na otu usoro onunu ogwu dị obere, a na-atụ aro ka ịchekwa na -20 Celsius C mgbe nkwakọchara.
3. Mgbe mmeghachi omume ahụ gasịrị, a na-edebe ihe ndị ahụ na 4 ℃ iji zere ifriizi ugboro ugboro.Emetụla ihe reagents tupu emeghachi omume iji zere mmetọ.
