Sistemụ njirimara DNA nke mbụ 30A (anaghị amịpụta)
Nkọwa ngwa:
Sistemụ njirimara DNA FOREGENE 30A na-eji teknụzụ akara fluorescent agba isiigbasaa 29autosomalSTR ebe, otuMpaghara Amel na otu mpaghara Yindel n'otu oge, ma nwee ike ịkwalite akwụkwọ nzacha ozugbo, FTAkaadị, owu swabs, kaadị saliva,na mpụọ swab.
Nkọwa
Sistemụ njirimara DNA FOREGENE 30A na-eji teknụzụ akara fluorescent agba isiigbasaa 29autosomalSTR ebe, otuMpaghara Amel na otu mpaghara Yindel n'otu oge, ma nwee ike ịkwalite akwụkwọ nzacha ozugbo, FTAkaadị, owu swabs, kaadị saliva,na mpụọ swab.
Ọdịnaya ngwa
Nkwadebe Sistemu Amplification
Tebụl 1: Nkwadebe nkwadebe nke mmeghachi omume nkwalite ọkọlọtọ (n'efu).
|
| Cndị na-akwado | 25 Sistemu μl (μl) | 10 Sistemu μl (μl) |
| Ngwakọta Master | 4 × Master Mix VII | 6.25 | 2.5 |
| 5 x 30ANgwakọta mbụ | 5.0 | 2 | |
| Mmiri a tọhapụrụ | 13.75 | 5.5 | |
| Blood unyi | Dayameta 1.2mm | Dayameta 1.2mm | |
| Tolu mmeghachi omume otal | 25 μl | 10 μl | |
Nyocha data
Eserese agbakwunyere 1:Map akara ubube Allelic
Eserese 2 agbakwunyere:Mpịnye DNA ọkọlọtọ 9948map
Nchekwa Reagent
1. Biko chekwaa n'okpuru -20 Celsius C,, mgbe ị natachara ngwa ndị a jụrụ oyi na ice akọrọ ma ọ bụ ngwugwu ice gel.
2. Biko chekwaa akụrụngwa pre-reaction 4 × premix VII na -20°C, mgbe ngwa ewepụtara maka iji, ma chekwaa ihe ndị ọzọ fọdụrụ tupu mmeghachi omume reagents na 4°C ka ịzenarị oyi na agbaze ugboro ugboro.Ọ bụrụ na otu usoro onunu ogwu dị obere, a na-atụ aro ka ị chekwaa ya na -20 Celsius C mgbe ịsachara ya.
3. Chekwaa ihe ndị ahụ mgbe mmeghachi omume ahụ gasịrị na 4 Celsius C, zere ịza oyi na thawing ugboro ugboro, ma emetụla reagents tupu mmeghachi omume iji zere mmetọ.
